无机化学学报
無機化學學報
무궤화학학보
JOURNAL OF INORGANIC CHEMISTRY
2007年
5期
849-853
,共5页
陈结霞%徐小龙%王守业%张立云%刘清亮
陳結霞%徐小龍%王守業%張立雲%劉清亮
진결하%서소룡%왕수업%장립운%류청량
抗血小板凝集素%二级结构%圆二色谱%拉曼光谱%红外光谱
抗血小闆凝集素%二級結構%圓二色譜%拉曼光譜%紅外光譜
항혈소판응집소%이급결구%원이색보%랍만광보%홍외광보
agkisacutacin%secondary structure%circular dichroism%Raman Resonance%FTIR
尖吻蝮蛇毒中抗血小板凝集素是凝血因子Ⅸ/凝血因子Ⅹ结合蛋白,它具有抗凝血和抑制血小板凝集双重活性.用红外光谱、拉曼光谱和CD谱研究了抗血小板凝集素的二级结构以及pH值和钙离子对其二级结构的影响.用CD谱测得,在水溶液中,抗血小板凝集素的主要骨架构象为β-折叠(26.3%)和α-螺旋(19.6%)结构.拉曼光谱显示,在粉末状态,其α-螺旋含量显著降低.CD谱还表明,抗血小板凝集素在pH值3.0~11.0范围内保持稳定的天然结构,钙离子诱导的抗血小板凝集素结构变化是可逆的,钙离子在稳定抗血小板凝集素的天然结构中起重要作用.
尖吻蝮蛇毒中抗血小闆凝集素是凝血因子Ⅸ/凝血因子Ⅹ結閤蛋白,它具有抗凝血和抑製血小闆凝集雙重活性.用紅外光譜、拉曼光譜和CD譜研究瞭抗血小闆凝集素的二級結構以及pH值和鈣離子對其二級結構的影響.用CD譜測得,在水溶液中,抗血小闆凝集素的主要骨架構象為β-摺疊(26.3%)和α-螺鏇(19.6%)結構.拉曼光譜顯示,在粉末狀態,其α-螺鏇含量顯著降低.CD譜還錶明,抗血小闆凝集素在pH值3.0~11.0範圍內保持穩定的天然結構,鈣離子誘導的抗血小闆凝集素結構變化是可逆的,鈣離子在穩定抗血小闆凝集素的天然結構中起重要作用.
첨문복사독중항혈소판응집소시응혈인자Ⅸ/응혈인자Ⅹ결합단백,타구유항응혈화억제혈소판응집쌍중활성.용홍외광보、랍만광보화CD보연구료항혈소판응집소적이급결구이급pH치화개리자대기이급결구적영향.용CD보측득,재수용액중,항혈소판응집소적주요골가구상위β-절첩(26.3%)화α-라선(19.6%)결구.랍만광보현시,재분말상태,기α-라선함량현저강저.CD보환표명,항혈소판응집소재pH치3.0~11.0범위내보지은정적천연결구,개리자유도적항혈소판응집소결구변화시가역적,개리자재은정항혈소판응집소적천연결구중기중요작용.
Agkisacutacin isolated from the venom of Agkistrodon acutus is a coagulation factor Ⅸ/coagulation factor Ⅹ-binding protein with marked anticoagulant- and platelet-modulating activities. The effects of pH value and Ca2+ ion on the secondary structure of Agkisacutacin have been studied by FTIR, Raman and circular dichroism (CD) spectroscopy. The main backbone conformations of holo-Agkisacutacin are α-helix (19.6%) and β-sheet (26.3%) as determined by CD spectroscopy. According to the Raman measurements, the content of α-helix of holo-Agkisacutacin in solid powder is less than that in solution. The conformation of Agkisacutacin is stable in the pH range of 3.0~11.0 as shown by CD spectroscopy. Ca2+-induced conformation change of Agkisacutacin is reversible. Ca2+ ion plays an important role in the stabilization of the structure of Agkisacutacin.