CAJ | 학술논문

研究了日本落叶松新鲜松针的化学成分及其抗氧化活性.采用Sephadex LH-20柱色谱及薄层色谱等方法进行分离,从其95%乙醇提取物乙酸乙酯溶性部分中分到7种化合物,经波谱分析及理化性质化合物分别鉴定为:儿茶素(1)、表儿茶素(2)、没食子儿茶素(3)、异鼠李素-3-O-葡萄糖苷(4)、紫云英苷(5)、2"-O-鼠李糖牡荆黄苷(6)和cedrusin (7).7种化合物均首次从该植物中分得.经DPPH试验,测定了核酸溶性部分、二氯甲烷溶性部分、乙酸乙酯溶性部分、水溶性部分、粗提物及分得化合物的抗氧化活性.其中乙酸乙酯溶性部分及化合物1～3具有很强的抗氧化活性.
연구료일본락협송신선송침적화학성분급기항양화활성.채용Sephadex LH-20주색보급박층색보등방법진행분리,종기95%을순제취물을산을지용성부분중분도7충화합물,경파보분석급이화성질화합물분별감정위:인다소(1)、표인다소(2)、몰식자인다소(3)、이서리소-3-O-포도당감(4)、자운영감(5)、2"-O-서리당모형황감(6)화cedrusin (7).7충화합물균수차종해식물중분득.경DPPH시험,측정료핵산용성부분、이록갑완용성부분、을산을지용성부분、수용성부분、조제물급분득화합물적항양화활성.기중을산을지용성부분급화합물1～3구유흔강적항양화활성.
Seven compounds, including three flavan-3-ols, (+)-catechin (1), (-)-epicatechin (2) and (+)-gallocatechin (3), three flavonoid glycosides, isorhamnetin-3-O-β-D-glucopyranoside (4), astragalin (5) and 2"-O-rhmnosylvitexin (6), and one lignan with the trivial name cedrusin (7), were isolated for the first time from the fresh needles of Larix kaempferi C. Their structures were elucidated by chemical and spectral analysis. The antioxidant activities of the crude extracts, partitioned fractions and isolated compounds were evaluated by DPPH free radicalscavenging assay. Results suggested that EtOAc participated fraction and the three flavan-3-ols showed significantly high antioxidant potential compared with α-tocopherol and BHT, which were used as controls.