食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2009年
7期
28-30
,共3页
库尔班·吾斯曼%穆赫塔尔·伊米尔艾山%帕提古丽·伊米尔艾山
庫爾班·吾斯曼%穆赫塔爾·伊米爾艾山%帕提古麗·伊米爾艾山
고이반·오사만%목혁탑이·이미이애산%파제고려·이미이애산
马齿苋子%多糖测定%抑菌活性
馬齒莧子%多糖測定%抑菌活性
마치현자%다당측정%억균활성
seed of Portulaca oleracea L%polysaccharide%bacteriostatic activities
采用苯酚-硫酸法,以葡萄糖作为标准品,分光光度计于490nm波长处测定其吸收度,对马齿苋子中多糖进行提取和含量分析并作抑菌活性试验.结果表明:多糖的线性范围为0.010 g/L～0.080 g/L,A=0.012 7C-0.003 2,r=0.999 0,平均加样回收率为98.01%,RSD=1.42%.多糖的提取率可达8.94%.抑菌活性试验结果表明,马齿苋子多糖除绿脓杆菌外其它三种对病菌有明显抑菌作用.
採用苯酚-硫痠法,以葡萄糖作為標準品,分光光度計于490nm波長處測定其吸收度,對馬齒莧子中多糖進行提取和含量分析併作抑菌活性試驗.結果錶明:多糖的線性範圍為0.010 g/L～0.080 g/L,A=0.012 7C-0.003 2,r=0.999 0,平均加樣迴收率為98.01%,RSD=1.42%.多糖的提取率可達8.94%.抑菌活性試驗結果錶明,馬齒莧子多糖除綠膿桿菌外其它三種對病菌有明顯抑菌作用.
채용분분-류산법,이포도당작위표준품,분광광도계우490nm파장처측정기흡수도,대마치현자중다당진행제취화함량분석병작억균활성시험.결과표명:다당적선성범위위0.010 g/L～0.080 g/L,A=0.012 7C-0.003 2,r=0.999 0,평균가양회수솔위98.01%,RSD=1.42%.다당적제취솔가체8.94%.억균활성시험결과표명,마치현자다당제록농간균외기타삼충대병균유명현억균작용.
Using phenol-sulfuric acid and UV spectra to determine the polysaccharide in 490nm and adopting the glucose for standard sample. To extract and bacteriostatic activities of polysaccharide in seed of Portulaca oleracea L. The content of polysaccharide had a good linearity in the range of 0.010 g/L-0.080 g/L, A=0.012 7 C-0.003 2,r=0.999 0.The average adds the kind recovery rate 98.01%,RSD=1.42%. Its large yield can reach to 8.94 %.The result of the bacteriostatic activities of the polysaccharide from seed of Portulaca oleracea has remarkable effect except pseudomonas aeruginosa.
