CAJ | 학술논문

目的研究白藜芦醇对脂多糖(LPS)致小鼠急性肺损伤(ALI)的保护作用,探讨其可能的作用机制.方法以小鼠气道滴注LPS制备急性肺损伤模型,检测气道吸气阻力(Ri)、气道呼气阻力(Re)和动态肺顺应性(Cdyn)的变化,测定支气管肺泡灌洗液(BALF)中白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)的含量,检测肺湿/干比值和毛细血管通透性,观察组织病理学变化.结果白藜芦醇能明显抑制Ri、Re增长和Cdyn降低,降低BALF中IL-1β、IL-6、TNF-α的含量,降低肺湿/干比值和渗透性,减轻肺组织病理学的损伤.结论白藜芦醇对LPS诱导的ALI具有保护作用,作用机制可能与抑制炎症因子的合成与释放有关.
목적 연구백려호순대지다당(LPS)치소서급성폐손상(ALI)적보호작용,탐토기가능적작용궤제.방법 이소서기도적주LPS제비급성폐손상모형,검측기도흡기조력(Ri)、기도호기조력(Re)화동태폐순응성(Cdyn)적변화,측정지기관폐포관세액(BALF)중백세포개소-1β(IL-1β)、백세포개소-6(IL-6)화종류배사인자-α(TNF-α)적함량,검측폐습/간비치화모세혈관통투성,관찰조직병이학변화.결과 백려호순능명현억제Ri、Re증장화Cdyn강저,강저BALF중IL-1β、IL-6、TNF-α적함량,강저폐습/간비치화삼투성,감경폐조직병이학적손상.결론 백려호순대LPS유도적ALI구유보호작용,작용궤제가능여억제염증인자적합성여석방유관.
Purpose To investigate the effect of resveratrol on acute lung injury induced by lipopolysaccharide (LPS) in mice.Methods Acute lung injury (ALI) was induced by LPS in mice. The changes of lung airway inspiratory resistance (Ri), expiratory resistance (Re), and dynamic lung compliance (Cdyn) were observed with pulmonary function test apparatus. Brochoalveolar lavage fluid (BALF) was collected, and the concentrations of interleukin 1β (IL-1β)、interleukin 6 (IL-6) and tumor necrosis factory α (TNF-α) were measured by ELISA. The ratio of wet to dry weight was calculated to assess lung edema. Pulmonary vascular permeability was examined with injection Evans blue to judge the destructive extent of alveolar epithelial cell and endothelial. Pathological section was made, and the histopathological change was observed with light microscope.Results Resveratrol can inhibit the elevation of Ri and Re, and the descent of Cdyn. Simultaneously, resveratrol reduced the concentration of IL-1β, IL-6 and TNF-α,as well as the wet to dry weight ratio and the pulmonary vascular permeability significantly. Furthermore, it also could attenuate the lung injury on histopathology.Conclusion The results show that pretreatment with resveratrol has a protective effect on ALI induced by LPS. The ultimate inhibiting and release of inflammatory factors were involved in the mechanism of the effects.