CAJ | 학술논문

实验采用GA_3、SNP、GA_3+L-NAME、GA_3+Na_2WO_4、GA_3+HB处理离体蒜苔基部,定期测定苔苞直径、可溶性总糖、抗氧化酶活性、膜透性、H_2O_2等指标,研究GA_3、NO在蒜苔衰老细胞内含物转运中的生理作用.结果表明:GA_3和NO均可抑制苔苞直径增大;提高超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)活性,顶部POD、CAT活性;抑制基部过氧化物酶(POD)、过氧化氢酶(CAT)活性、H_2O_2和可溶性糖含量下降以及膜透性的升高;清除NO或抑制内源NO来源的任何一条途径均对GA_3和NO的作用结果有影响,由此表明GA_3延缓蒜苔物质转运可能通过NO起作用.
실험채용GA_3、SNP、GA_3+L-NAME、GA_3+Na_2WO_4、GA_3+HB처리리체산태기부,정기측정태포직경、가용성총당、항양화매활성、막투성、H_2O_2등지표,연구GA_3、NO재산태쇠로세포내함물전운중적생리작용.결과표명:GA_3화NO균가억제태포직경증대;제고초양화물기화매(SOD)、항배혈산과양화물매(APX)활성,정부POD、CAT활성;억제기부과양화물매(POD)、과양화경매(CAT)활성、H_2O_2화가용성당함량하강이급막투성적승고;청제NO혹억제내원NO래원적임하일조도경균대GA_3화NO적작용결과유영향,유차표명GA_3연완산태물질전운가능통과NO기작용.
In order to research the physiological mechanism of GA_3 and NO in contents edistribution of cell, the basal part of garlic scape was treated with GA_3, SNP, GA_3 + L-NAME,GA_3+ Na_2WO_4, GA_3+ HB for three hours, respectively. The resulting samples were air dried and then stored at 4℃. The diameter of garlic bulbet, the content of total soluble sugar, antioxidant enzyme activity and other indices were measured regularly. The result indicated that the diameter of garlic bulbet, the activities of POD and CAT, membrane permeability,the content of soluble sugar in the basal part of garlic scape were inhibited by GA_3 and SNP, respectively. The activities of SOD and APX in the garlic scape and the activities of POD and CAT in the apical part of garlic scape were promoted by treatment of GA_3 and SNP. If eliminated or suppressed NO in any way could affect GA_3 and SNP, so GA_3 may restraint the redistribution of nutrients transport by NO.