CAJ | 학술논문

利用HE染色方法对拟黑多刺蚁卵子发生进行显微研究.结果发现,拟黑多刺蚁卵子发生可以分为3个时期11个阶段,即卵母细胞分化期、卵母细胞生长期和卵母细胞卵黄形成期.在卵子发生的整个过程中,卵母细胞、滋养细胞及滤泡细胞形态均有明显变化;在卵母细胞生长及卵黄形成期.滋养细胞核内的多核仁现象、灯刷染色体与旺盛的物质合成有关;卵母细胞本身也合成一些物质:滤泡细胞参与卵黄蛋白的合成,并为外源性卵黄蛋白提供通道.因此,卵母细胞内卵黄蛋白的形成是自体合成和外源合成同时并存;滤泡细胞还参与了卵黄膜及卵壳的形成.并发现,拟黑多刺蚁与果蝇卵子发生有相似的卵母细胞凋亡现象,主要发生在卵黄形成前期,这种凋亡可能是由于环境因素的影响,即生物对不良环境的适应性对策.
이용HE염색방법대의흑다자의란자발생진행현미연구.결과발현,의흑다자의란자발생가이분위3개시기11개계단,즉란모세포분화기、란모세포생장기화란모세포란황형성기.재란자발생적정개과정중,란모세포、자양세포급려포세포형태균유명현변화;재란모세포생장급란황형성기.자양세포핵내적다핵인현상、등쇄염색체여왕성적물질합성유관;란모세포본신야합성일사물질:려포세포삼여란황단백적합성,병위외원성란황단백제공통도.인차,란모세포내란황단백적형성시자체합성화외원합성동시병존;려포세포환삼여료란황막급란각적형성.병발현,의흑다자의여과승란자발생유상사적란모세포조망현상,주요발생재란황형성전기,저충조망가능시유우배경인소적영향,즉생물대불량배경적괄응성대책.
HE staining method was used to study Polyrhachis vicina Roger ant oogenesis.The results showed that the ant oogenesis could be divided into 3 phases 11 stages.3 phases is composed of oocyte differentiation phase,the oocyte growth phase of yolk formation.In the whole process,the oocytes,nurse cell and follicle cell morphology changed.In oocyte yolk formation and growth,the number of its nuclear trophoblast cell nucleoli,lampbrush chromosome associated with a strong synthetic material;oocytes also synthesed some materials;follicle cells took part in the yolk protein synthesis,provided access to exogenous yolk protein.Therefore,oocytes from the yolk protein synthesis and the formation of exogenous synthetic coexisted;follicle cell was also involved in the formation of a shell egg yolk membrane.And it was found that oogenesis in Drosophila and ant was similar to the oocyte apoptosis.Yolk mainly occurred in the early stage of apoptosis,which might be due to environmental factors and be the adaptability of the adverse environmental measures.