CAJ | 학술논문

目标检测白细胞介素-12(IL-12)在系统性红斑狼疮(SLE)患者体外培养的外周血单个核细胞的表达及对STAT3和STAT4信号传导分子的作用.方法采用RT-PCR和免疫印迹分别对39例SLE病人和11例正常人进行对照研究,培养的细胞上清液中的IL-12水平应用ELISA法检测.结果 IL-12的蛋白和基因水平在狼疮活动及静止期均较正常对照高,PHA能促进IL-12的表达.IL-12能够单独诱导STAT3和STAT4的活化,尤其是在SLE活动期;然而IL-12对正常PBMC没有作用;以IL-12+PHA处理正常PBMC时,可以观察到STAT3和STAT4的活化.结论 IL-12可通过异常磷酸化的STAT3和STAT4信号分子在SLE中发挥生物学作用.
목표검측백세포개소-12(IL-12)재계통성홍반랑창(SLE)환자체외배양적외주혈단개핵세포적표체급대STAT3화STAT4신호전도분자적작용.방법채용RT-PCR화면역인적분별대39례SLE병인화11례정상인진행대조연구,배양적세포상청액중적IL-12수평응용ELISA법검측.결과 IL-12적단백화기인수평재랑창활동급정지기균교정상대조고,PHA능촉진IL-12적표체.IL-12능구단독유도STAT3화STAT4적활화,우기시재SLE활동기;연이IL-12대정상PBMC몰유작용;이IL-12+PHA처리정상PBMC시,가이관찰도STAT3화STAT4적활화.결론 IL-12가통과이상린산화적STAT3화STAT4신호분자재SLE중발휘생물학작용.
Objective To determine the in vitro expression of interleukin-12 (IL-12) and its effect on signal transducers and activators of transcription (STAT) signaling molecules in peripheral blood mononuclear cells (PBMCs) in patients with systemic lupus erythematosus (SLE).Methods Peripheral blood mononuclear cells in 39 patients with definite systemic lupus erythematosus and 11 healthy volunteers were collected. Expression of IL-12 P40mRNA in PBMCs was determined with reverse transcription-polymerase chain reaction (RT-PCR). Quantity of IL-12 protein supernatant was measured by enzyme-linked immunosorbent assay (ELISA). The levels of phosphorylated STAT3 and STAT4 signaling molecules in PBMCs were detected by immunoblot. Results Levels of IL-12 protein and mRNA expression in patients with active or inactive SLE were significantly higher than those in controls. Phytohemagglutinin (PHA) may promote the expression of IL-12. IL-12 alone induced the phosphorylation of STAT3 and STAT4 in PBMCs from patients with SLE, especially in active SLE. However it had no obvious effect on normal PBMCs. Phosphorylated STAT3 and STAT4 might be observed in normal PBMCs treated with IL-12 plus PHA.Conclusion IL-12 is produced aberrantly in patients with SLE. IL-12 might exert its biological role in SLE via the aberrantly phosphorylated STAT3 and STAT4 signaling molecules.