CAJ | 학술논문

目的研究苯扎贝特对氧化型低密度脂蛋白(ox-LDL)诱导人脐静脉内皮细胞(HUVECs)凋亡的影响及血凝素样氧化型低密度脂蛋白受体1(LOX-1)在其中的作用.方法用流式细胞仪观察HUVECs凋亡,用RT-PCR技术观察LOX-1基因表达的变化.结果 ox-LDL组与正常组比较凋亡明显增加(P<0.05),LOX-1 mRNA表达明显增加(P<0.05),苯扎贝特干预后凋亡减少,LOX-1 mRNA的表达降低(P<0.05),呈浓度依赖关系.用LOX-1阻滞剂多聚肌苷酸预先干预2 h后与苯扎贝特组比较细胞凋亡及LOX-1mRNA表达进一步减少(P<0.05).结论苯扎贝特可改善ox-LDL对内皮细胞的影响,下调LOX-1表达,减少内皮细胞凋亡,部分机制可能通过LOX-1介导.
목적 연구분찰패특대양화형저밀도지단백(ox-LDL)유도인제정맥내피세포(HUVECs)조망적영향급혈응소양양화형저밀도지단백수체1(LOX-1)재기중적작용.방법 용류식세포의관찰HUVECs조망,용RT-PCR기술관찰LOX-1기인표체적변화.결과 ox-LDL조여정상조비교조망명현증가(P<0.05),LOX-1 mRNA표체명현증가(P<0.05),분찰패특간예후조망감소,LOX-1 mRNA적표체강저(P<0.05),정농도의뢰관계.용LOX-1조체제다취기감산예선간예2 h후여분찰패특조비교세포조망급LOX-1mRNA표체진일보감소(P<0.05).결론 분찰패특가개선ox-LDL대내피세포적영향,하조LOX-1표체,감소내피세포조망,부분궤제가능통과LOX-1개도.
Objective To observe the influence of bezafibrate on the apoptosis and expression of lectin-like oxidized low density lipoprotein receptor-1(LOX-1) mediated by oxidized low density lipoprotein(ox-LDL) in cultured human umbilical vein endothelial cells(HUVECs).Methodes The apoptosis of HUVECs mediated by ox-LDL were evaluated by flow cytometry and the expression of LOX-1 mRNA were detected by RT-PCR.Results Compared with the control group,ox-LDL could increase apoptosis and the expression of LOX-1(P<0.05),and Bezafibrate could decrease the apoptosis and the expression of LOX-1 in a concentration -dependent manner(P<0.05).Preincubation of HUVECs with polyinosinic acid for 2 hours,the apoptosis and the expression of LOX-1 decreased(P<0.05).Conclusions Bezafibrate inhibits the apoptosis of HUVECs mediated by ox-LDL by reducing the expression of LOX-1,which may be part of the reasons for bezafibrate to prevent and treat atherosclerosis.