国际脑血管病杂志
國際腦血管病雜誌
국제뇌혈관병잡지
INTERNATIONAL JOURNAL OF CEREBROVASCULAR DISEASES
2011年
2期
95-100
,共6页
血小板膜糖蛋白%P-选择素%血小板因子4%卒中%脑缺血%脑梗死%动脉粥样硬化
血小闆膜糖蛋白%P-選擇素%血小闆因子4%卒中%腦缺血%腦梗死%動脈粥樣硬化
혈소판막당단백%P-선택소%혈소판인자4%졸중%뇌결혈%뇌경사%동맥죽양경화
Platelet membrane glycoproteins%P-selectin%Platelet factor 4%Stroke%Brain ischemia%Brain infarction%Atherosclerosis
目的 探讨脑小血管病及其亚型与大动脉粥样硬化性卒中患者血小板膜糖蛋白PAC-1和CD62P表达的差异,并对传统血小板活化标记物和炎症趋化因子血小板因子4(platelet factor 4,PF4)进行比较.方法 分别采用流式细胞术和酶联免疫吸附法测定30例大动脉粥样硬化性卒中患者、45例脑小血管病患者和30例对照者外周血血小板PAC-1、CD62P阳性率以及血清PF4浓度,比较其组间差异.结果 大动脉粥样硬化性卒中组PAC-1、CD62P阳性率和PF4浓度分别为(63.21±9.78)%、(55.91±8.17)%和(30.55±15.56)ng/ml显著高于脑小血管病组的(40.65±17.42)%、(36.49±14.60)%和(12.59±5.57)ng/ml(P均=0.000),而后者仍高于对照组的(13.55±3.14)%、(9.oo±2.32)%和(4.95±2.82)ng/ml(P均=0.000).PAC-1、CD62P阳性率和PF4浓度在脑小血管病各亚组间存在显著差异,脑白质疏松合并腔隙性梗死组[(47.72±15.52)%、(43.75±12.54)%和(13.96±5.23)ng/ml]和单纯腔隙性梗死组[(49.87±14.65)%、(43.98±10.55)%和(14.41±5.53)ng/ml]显著高于单纯脑白质疏松组[(24.44±8.45)%、(21.74±7.19)%和(9.40±4.99)ng/ml](P分别为o.000、0.000和0.013),而前两组间无显著差异(P分别为0.658、0.952和0.858).缺血性脑血管病患者外周血PAC-1与CD62P阳性率显著相关(r=0.964,P=0.000),且血清PF4浓度分别与PAC-1(r=0.846,P=0.000)和CD62P(r=0.857,P=0.000)阳性率均呈直线相关.结论 血小板膜糖蛋白PAC-1与CD62P表达呈线性相关,并且与PF4浓度变化一致,提示血小板活化及其介导的炎症机制在动脉粥样硬化和血栓形成过程中起着重要的病理生理学作用,该机制在不同病变类型之间存在显著差异.
目的 探討腦小血管病及其亞型與大動脈粥樣硬化性卒中患者血小闆膜糖蛋白PAC-1和CD62P錶達的差異,併對傳統血小闆活化標記物和炎癥趨化因子血小闆因子4(platelet factor 4,PF4)進行比較.方法 分彆採用流式細胞術和酶聯免疫吸附法測定30例大動脈粥樣硬化性卒中患者、45例腦小血管病患者和30例對照者外週血血小闆PAC-1、CD62P暘性率以及血清PF4濃度,比較其組間差異.結果 大動脈粥樣硬化性卒中組PAC-1、CD62P暘性率和PF4濃度分彆為(63.21±9.78)%、(55.91±8.17)%和(30.55±15.56)ng/ml顯著高于腦小血管病組的(40.65±17.42)%、(36.49±14.60)%和(12.59±5.57)ng/ml(P均=0.000),而後者仍高于對照組的(13.55±3.14)%、(9.oo±2.32)%和(4.95±2.82)ng/ml(P均=0.000).PAC-1、CD62P暘性率和PF4濃度在腦小血管病各亞組間存在顯著差異,腦白質疏鬆閤併腔隙性梗死組[(47.72±15.52)%、(43.75±12.54)%和(13.96±5.23)ng/ml]和單純腔隙性梗死組[(49.87±14.65)%、(43.98±10.55)%和(14.41±5.53)ng/ml]顯著高于單純腦白質疏鬆組[(24.44±8.45)%、(21.74±7.19)%和(9.40±4.99)ng/ml](P分彆為o.000、0.000和0.013),而前兩組間無顯著差異(P分彆為0.658、0.952和0.858).缺血性腦血管病患者外週血PAC-1與CD62P暘性率顯著相關(r=0.964,P=0.000),且血清PF4濃度分彆與PAC-1(r=0.846,P=0.000)和CD62P(r=0.857,P=0.000)暘性率均呈直線相關.結論 血小闆膜糖蛋白PAC-1與CD62P錶達呈線性相關,併且與PF4濃度變化一緻,提示血小闆活化及其介導的炎癥機製在動脈粥樣硬化和血栓形成過程中起著重要的病理生理學作用,該機製在不同病變類型之間存在顯著差異.
목적 탐토뇌소혈관병급기아형여대동맥죽양경화성졸중환자혈소판막당단백PAC-1화CD62P표체적차이,병대전통혈소판활화표기물화염증추화인자혈소판인자4(platelet factor 4,PF4)진행비교.방법 분별채용류식세포술화매련면역흡부법측정30례대동맥죽양경화성졸중환자、45례뇌소혈관병환자화30례대조자외주혈혈소판PAC-1、CD62P양성솔이급혈청PF4농도,비교기조간차이.결과 대동맥죽양경화성졸중조PAC-1、CD62P양성솔화PF4농도분별위(63.21±9.78)%、(55.91±8.17)%화(30.55±15.56)ng/ml현저고우뇌소혈관병조적(40.65±17.42)%、(36.49±14.60)%화(12.59±5.57)ng/ml(P균=0.000),이후자잉고우대조조적(13.55±3.14)%、(9.oo±2.32)%화(4.95±2.82)ng/ml(P균=0.000).PAC-1、CD62P양성솔화PF4농도재뇌소혈관병각아조간존재현저차이,뇌백질소송합병강극성경사조[(47.72±15.52)%、(43.75±12.54)%화(13.96±5.23)ng/ml]화단순강극성경사조[(49.87±14.65)%、(43.98±10.55)%화(14.41±5.53)ng/ml]현저고우단순뇌백질소송조[(24.44±8.45)%、(21.74±7.19)%화(9.40±4.99)ng/ml](P분별위o.000、0.000화0.013),이전량조간무현저차이(P분별위0.658、0.952화0.858).결혈성뇌혈관병환자외주혈PAC-1여CD62P양성솔현저상관(r=0.964,P=0.000),차혈청PF4농도분별여PAC-1(r=0.846,P=0.000)화CD62P(r=0.857,P=0.000)양성솔균정직선상관.결론 혈소판막당단백PAC-1여CD62P표체정선성상관,병차여PF4농도변화일치,제시혈소판활화급기개도적염증궤제재동맥죽양경화화혈전형성과정중기착중요적병리생이학작용,해궤제재불동병변류형지간존재현저차이.
Objective To investigate the differences of platelet membrane glycoprotein PAC-1 and CD62P expression in patients with cerebral small vessel disease and its subtype and large artery atherosclerotic stroke and to compare the traditional platelet activation markers and inflammatory chemokine platelet factor 4(PF4).Methods Peripheral blood platelet PAC-1,CD62P positive rates and serum PF4 concentration in 30 patients with large artery atherosclerotic stroke,45 patients with cerebral small vessel disease and 30 controls were detected using flow cytometry and enzyme-linked immunosorbent assay.The differences between the groups were compared.Results The PAC-1,CD62P positive rates and serum PF4 concentration in the large artery atherosclerotic stroke group were 63.21% ± 9.78%,55.91% ± 8.17%,and 30.55 ± 15.56 ng/ml,respectively.They were significantly higher than 40.65% ± 17.42%,36.49% ± 14.60%,and 12.59 ±5.57 ng/mlin the cerebral small vessel disease group(all P=0.000),and the latter was still higher than 13.55% ± 3.14%,9.00% ± 2.32%,and 4.95 ±2.82 ng/ml in the control group(all P = 0.000).There were significant differences in all the subtypes groups of cerebral small vessel disease between the PAC-1,CD62P positive rates and serum PF4 concentration.The leukoaraiosis with lacunar infarction group(n = 15;47.72% ±15.52%,43.75% ± 12.54%,and 13.96 ± 5.23 ng/ml)and the simple lacunar infarction group (n = 15;49.87% ± 14.65%,43.98% ± 10.55%,and 14.41 ± 5.53 ng/ml)was significantly higher than the simple lacmar infarction group(n = 15;24.44% ± 8.45%,21.74% ± 7.19%,and 9.40 ±4.99 ng/ml)(P =0.000,0.000,and 0.013,respectively).There was no significant difference between the forrner 2 groups(P = 0.658,0.952,and 0.858,respectively).The peripheral blood platelet PAC-1 positive rate had significant correlation with CD62P positive rate in patients with ischemic cerebral disease(r= 0.964,P= 0.000),and the serum PF4 concentration showed linier correlation with the PAC-1(r =0.846,P =0.000)and CD62P(r =0.857,P =0.000)positive rates.Conelusions The platelet membrane glycoprotein PAC-1 and CD62P expression showed linear correlation,and they were consistent with the changes of PF4 concentration.This sugested that platelet activation and its mediated inflammatory mechanisms played an important pathophysiological role in the processes of atherosclerosis and thrombosis.This mechanism had significant difference between the different lesion types.
