中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2011年
2期
193-196
,共4页
顺铂%高温,诱发%细胞系,肿瘤%红细胞%血液
順鉑%高溫,誘髮%細胞繫,腫瘤%紅細胞%血液
순박%고온,유발%세포계,종류%홍세포%혈액
Cisplatin%Hyperthermia,induced%Cell line,tumor%Erythrocytes%Blood
目的 探讨顺铂(DDP)联合加热对体外血液中肝肿瘤细胞的杀灭作用及对红细胞的影响.方法 将肝肿瘤细胞2 ml加入10 ml红细胞悬液中,充分混匀后,分别取2 ml于离心管中.采用随机数字表法,将其随机分为6组(n=30):A组、D组各加入2 ml生理盐水,B组、E组各加入2 mlDDP(终浓度为100 μg/ml),C组和F组各加入2 ml DDP(终浓度为200 μg/ml).充分混匀后,A组、B组和C组放入37℃恒温水浴箱中,其余3组放入42℃恒温水浴箱中进行加热处理,处理时间均为30min.采用密度梯度离心法分离肝肿瘤细胞和红细胞,测定肝肿瘤细胞抑制率及克隆形成率,测定红细胞渗透脆性及2,3-二磷酸甘油酸(2,3-DPG)含量.结果 随温度和DDP浓度的升高,肿瘤细胞的抑制率升高,克隆形成率降低(P<0.01).F组肿瘤细胞抑制率达98%以上,且未出现克隆形成.处理前后F组红细胞2,3-DPG含量比较差异无统计学意义(P->0.05).处理后F组红细胞在0.68%NaCl溶液中的溶血率<1%.结论 200 μg/ml DDP联合42 ℃加热30 min,可使体外血液中肝肿瘤细胞失去增殖能力,而对红细胞膜的影响轻微,对红细胞的携氧能力无影响.
目的 探討順鉑(DDP)聯閤加熱對體外血液中肝腫瘤細胞的殺滅作用及對紅細胞的影響.方法 將肝腫瘤細胞2 ml加入10 ml紅細胞懸液中,充分混勻後,分彆取2 ml于離心管中.採用隨機數字錶法,將其隨機分為6組(n=30):A組、D組各加入2 ml生理鹽水,B組、E組各加入2 mlDDP(終濃度為100 μg/ml),C組和F組各加入2 ml DDP(終濃度為200 μg/ml).充分混勻後,A組、B組和C組放入37℃恆溫水浴箱中,其餘3組放入42℃恆溫水浴箱中進行加熱處理,處理時間均為30min.採用密度梯度離心法分離肝腫瘤細胞和紅細胞,測定肝腫瘤細胞抑製率及剋隆形成率,測定紅細胞滲透脆性及2,3-二燐痠甘油痠(2,3-DPG)含量.結果 隨溫度和DDP濃度的升高,腫瘤細胞的抑製率升高,剋隆形成率降低(P<0.01).F組腫瘤細胞抑製率達98%以上,且未齣現剋隆形成.處理前後F組紅細胞2,3-DPG含量比較差異無統計學意義(P->0.05).處理後F組紅細胞在0.68%NaCl溶液中的溶血率<1%.結論 200 μg/ml DDP聯閤42 ℃加熱30 min,可使體外血液中肝腫瘤細胞失去增殖能力,而對紅細胞膜的影響輕微,對紅細胞的攜氧能力無影響.
목적 탐토순박(DDP)연합가열대체외혈액중간종류세포적살멸작용급대홍세포적영향.방법 장간종류세포2 ml가입10 ml홍세포현액중,충분혼균후,분별취2 ml우리심관중.채용수궤수자표법,장기수궤분위6조(n=30):A조、D조각가입2 ml생리염수,B조、E조각가입2 mlDDP(종농도위100 μg/ml),C조화F조각가입2 ml DDP(종농도위200 μg/ml).충분혼균후,A조、B조화C조방입37℃항온수욕상중,기여3조방입42℃항온수욕상중진행가열처리,처리시간균위30min.채용밀도제도리심법분리간종류세포화홍세포,측정간종류세포억제솔급극륭형성솔,측정홍세포삼투취성급2,3-이린산감유산(2,3-DPG)함량.결과 수온도화DDP농도적승고,종류세포적억제솔승고,극륭형성솔강저(P<0.01).F조종류세포억제솔체98%이상,차미출현극륭형성.처리전후F조홍세포2,3-DPG함량비교차이무통계학의의(P->0.05).처리후F조홍세포재0.68%NaCl용액중적용혈솔<1%.결론 200 μg/ml DDP연합42 ℃가열30 min,가사체외혈액중간종류세포실거증식능력,이대홍세포막적영향경미,대홍세포적휴양능력무영향.
Objective To investigate the effectiveness of cis-diamminedichloroplatinum (DDP) combined with hyperthennia in killing liver tumor cells and its influence on erythrocytes in vitro. Methods Cultured liver tumor cells (2 ml) were mixed with erythrocyte suspension (10 ml) and then the mixture was separated into 6 centrifuge tubes with 2 ml in each one. The centrifuge tubes were randomly divided into A-F groups and the experiment was repeated for 30 times. Normal saline 2 ml was added in A and D groups. DDP 2 ml (200 μg/ml) was added in B and E groups. DDP 2 ml (400 μg/ml) was added in C and F groups. The cells were then incubated in warm bath of 37 ℃ for 30 min in A, B and C groups and in warm bath of 42 ℃ for 30 min in the other three groups.After hyperthermic treatment, tumor cells were isolated from erythrocytes using density gradient centrifugation, the inhibition rate of tumor cells was determined by MTT method and the clone formation of tumor cells was checked.The erythrocyte osmotic fragility and content of 2,3-diphosphoglyceric acid in erythrocytes were measured. Results The inhibition rate of tumor cells was gradually increased, while the rate of tumor cell clone formation decreased with the increase in the temperature and DDP concentrations ( P < 0.01) . The rate of tumor cell clone formation was more than 98% and no clone formation was tested in group F. There was no significant difference in the content of 2,3-diphosphoglyceric acid in erythrocytes between before and after hyperthermic treatment in group F ( P >0.05 ) . The rate of hemolysis of erythrocytes was less than 1 % in the 0.68 % sodium chloride solution in group F.Conclusion DDP 200 μg/ml combined with hyperthermic treatment with temperature of 42 ℃ for 30 min can make the liver tumor cells lose the capability of proliferation, however, it exerts slight effect on erythrocyte membrane and no influence on the oxygen-carrying capacity of erythrocytes.
