中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2010年
7期
416-420
,共5页
陈伟强%冯凤兰%古宏标%潘德顺
陳偉彊%馮鳳蘭%古宏標%潘德順
진위강%풍봉란%고굉표%반덕순
癌基因蛋白p21%舌肿瘤%癌,鳞状细胞
癌基因蛋白p21%舌腫瘤%癌,鱗狀細胞
암기인단백p21%설종류%암,린상세포
Oncogene protein p21%Tongue neoplasms%Carcinoma,squamous cell
目的 探讨苯丁酸钠对人舌鳞状细胞癌Tca8113和TCSSA细胞株的生长、凋亡的影响及其分子机制,为临床治疗提供理论依据.方法 应用甲基噻唑基四唑(MTT)法检测苯丁酸钠对人舌鳞状细胞癌细胞株的抑制作用,采用流式细胞仪研究苯丁酸钠对人舌鳞状细胞癌细胞株的细胞周期阻滞和诱导凋亡作用,应用蛋白质印迹法和反转录聚合酶链反应(RT-PCR)检测p21和生存素基因的转录和表达.结果 苯丁酸钠对两种细胞株增殖均有明显的抑制作用;流式细胞仪检测显示苯丁酸钠诱导细胞株G1期阻滞,碘化丙啶-异硫氰酸荧光素标记的磷脂酰结合蛋白双标结果表明,苯丁酸钠能诱导细胞株凋亡,使p21的mRNA及蛋白质表达升高,与对照组比较,Tca8113细胞株的p21 mRNA及蛋白质分别增加0.09±0.08和0.72±0.10,TCSSA细胞株的p21mRNA及蛋白质分别增加1.34 4±0.12和1.56±0.09(P<0.05).生存索的mRNA及蛋白质表达下降.与对照组比较,Tca8113细胞株的生存素mRNA及蛋白质分别降低1.10±0.05和1.14±1.10,TCSSA细胞株的生存素mRNA及蛋白质分别降低1.02±0.08和0.94±0.09(P<0.05).苯丁酸钠诱导p21表达上升和生存素表达下降,二者的mRNA水平变化呈显著负相关(rs=-0.532,P<0.001),蛋白表达变化同样呈显著负相关(rs=-0.564,P<0.001).结论 苯丁酸钠能抑制人舌鳞状细胞癌细胞株增殖,诱导细胞G1期阻滞和细胞凋亡.
目的 探討苯丁痠鈉對人舌鱗狀細胞癌Tca8113和TCSSA細胞株的生長、凋亡的影響及其分子機製,為臨床治療提供理論依據.方法 應用甲基噻唑基四唑(MTT)法檢測苯丁痠鈉對人舌鱗狀細胞癌細胞株的抑製作用,採用流式細胞儀研究苯丁痠鈉對人舌鱗狀細胞癌細胞株的細胞週期阻滯和誘導凋亡作用,應用蛋白質印跡法和反轉錄聚閤酶鏈反應(RT-PCR)檢測p21和生存素基因的轉錄和錶達.結果 苯丁痠鈉對兩種細胞株增殖均有明顯的抑製作用;流式細胞儀檢測顯示苯丁痠鈉誘導細胞株G1期阻滯,碘化丙啶-異硫氰痠熒光素標記的燐脂酰結閤蛋白雙標結果錶明,苯丁痠鈉能誘導細胞株凋亡,使p21的mRNA及蛋白質錶達升高,與對照組比較,Tca8113細胞株的p21 mRNA及蛋白質分彆增加0.09±0.08和0.72±0.10,TCSSA細胞株的p21mRNA及蛋白質分彆增加1.34 4±0.12和1.56±0.09(P<0.05).生存索的mRNA及蛋白質錶達下降.與對照組比較,Tca8113細胞株的生存素mRNA及蛋白質分彆降低1.10±0.05和1.14±1.10,TCSSA細胞株的生存素mRNA及蛋白質分彆降低1.02±0.08和0.94±0.09(P<0.05).苯丁痠鈉誘導p21錶達上升和生存素錶達下降,二者的mRNA水平變化呈顯著負相關(rs=-0.532,P<0.001),蛋白錶達變化同樣呈顯著負相關(rs=-0.564,P<0.001).結論 苯丁痠鈉能抑製人舌鱗狀細胞癌細胞株增殖,誘導細胞G1期阻滯和細胞凋亡.
목적 탐토분정산납대인설린상세포암Tca8113화TCSSA세포주적생장、조망적영향급기분자궤제,위림상치료제공이론의거.방법 응용갑기새서기사서(MTT)법검측분정산납대인설린상세포암세포주적억제작용,채용류식세포의연구분정산납대인설린상세포암세포주적세포주기조체화유도조망작용,응용단백질인적법화반전록취합매련반응(RT-PCR)검측p21화생존소기인적전록화표체.결과 분정산납대량충세포주증식균유명현적억제작용;류식세포의검측현시분정산납유도세포주G1기조체,전화병정-이류청산형광소표기적린지선결합단백쌍표결과표명,분정산납능유도세포주조망,사p21적mRNA급단백질표체승고,여대조조비교,Tca8113세포주적p21 mRNA급단백질분별증가0.09±0.08화0.72±0.10,TCSSA세포주적p21mRNA급단백질분별증가1.34 4±0.12화1.56±0.09(P<0.05).생존색적mRNA급단백질표체하강.여대조조비교,Tca8113세포주적생존소mRNA급단백질분별강저1.10±0.05화1.14±1.10,TCSSA세포주적생존소mRNA급단백질분별강저1.02±0.08화0.94±0.09(P<0.05).분정산납유도p21표체상승화생존소표체하강,이자적mRNA수평변화정현저부상관(rs=-0.532,P<0.001),단백표체변화동양정현저부상관(rs=-0.564,P<0.001).결론 분정산납능억제인설린상세포암세포주증식,유도세포G1기조체화세포조망.
Objective To examine the effects of sodium phenylbutyrate on the apoptosis of human tongue squamous cancer cell line and expression of p21 and survivin genes.Methods The inhibition effects of sodium phenylbutyrate on Tca8113 and human tongue squamous cell carcinoma (TCSSA) cell lines were detected by methyl thiazoly terazolium (MTT) and the apoptosis of the cancer cells after being induced by sodium phenylbutyrate examined by flow cytometry(FCM).The expression of p21 and survivin genes were observed with Western blotting and RT-PCR Results Compared with control group,the level of p21 mRNA and protein of Tca8113 cellline increased to 0.09±0.08 and increased 0.72±0.10,that of TCSSA cellline increased 1.34±0.12 and 1.56±0.09(P <0.05).Compared with control group,the level of surrive mRNA and protein of Tca8113 cellline decreased to 1.10±0.05 and 1.14±1.10,that of TCSSA cellline decreased to 0.12±0.08 and 0.94±0.09 (P < 0.05) .Sodium phenylbutyrate inhibited the cell proliferation ,promoted cell apoptosis and arrested the cells in G1/G0 phase.The amount of p21 mRNA and protein were increased,and the expression of survivin gene was decreased.Conclusions Sodium phenylbutyrate exhibited remarkable inhibitory effects on human tongue squamous cancer cell proliferation and induced cancer cell apoptosis.The mechanism may be due to up-regulation of p21 gene and downregulation of survivin gene.The mRNA level of p21 gene and survivin gene showed a strong correlation.
