中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2010年
4期
260-262
,共3页
王凤龙%江建明%肖军%杨德鸿%王飞%付兆宗
王鳳龍%江建明%肖軍%楊德鴻%王飛%付兆宗
왕봉룡%강건명%초군%양덕홍%왕비%부조종
骨关节炎%白细胞介素18%滑膜细胞%细胞因子
骨關節炎%白細胞介素18%滑膜細胞%細胞因子
골관절염%백세포개소18%활막세포%세포인자
Osteoarthritis%Interleukin-18%Synoviocyte%Cytokine
目的 了解白细胞介素(IL)-18在骨关节炎(OA)患者和非OA患者滑膜细胞中表达的差异.方法 取OA患者(22例)与非OA患者(8例)关节滑膜组织,分别原代培养滑膜细胞,采用酶联免疫吸附测定法(ELISA)定量检测2组滑膜细胞培养上清液中IL-18蛋白的表达水平,采用免疫细胞化学方法 定性观察IL-18在2组滑膜细胞问表达的差异,统计学处理采用t检验.结果 ELISA检测OA患者细胞培养上清液中IL-18的含量为(63.9±21.4)pg/ml, 非OA患者细胞培养上清液中IL-18的含量为(17.7±1.5)pg/ml,2组比较差异有统计学意义(t=10.044,P<0.01);免疫细胞化学方法 显示在OA患者滑膜细胞中IL-18蛋白的表达呈阳性.胞质为棕黄色深染,而在非OA患者滑膜细胞中胞质淡染.结论 IL-18在OA关节滑膜细胞中表达上调, 提示IL-18可能直接参与了OA的发病过程.
目的 瞭解白細胞介素(IL)-18在骨關節炎(OA)患者和非OA患者滑膜細胞中錶達的差異.方法 取OA患者(22例)與非OA患者(8例)關節滑膜組織,分彆原代培養滑膜細胞,採用酶聯免疫吸附測定法(ELISA)定量檢測2組滑膜細胞培養上清液中IL-18蛋白的錶達水平,採用免疫細胞化學方法 定性觀察IL-18在2組滑膜細胞問錶達的差異,統計學處理採用t檢驗.結果 ELISA檢測OA患者細胞培養上清液中IL-18的含量為(63.9±21.4)pg/ml, 非OA患者細胞培養上清液中IL-18的含量為(17.7±1.5)pg/ml,2組比較差異有統計學意義(t=10.044,P<0.01);免疫細胞化學方法 顯示在OA患者滑膜細胞中IL-18蛋白的錶達呈暘性.胞質為棕黃色深染,而在非OA患者滑膜細胞中胞質淡染.結論 IL-18在OA關節滑膜細胞中錶達上調, 提示IL-18可能直接參與瞭OA的髮病過程.
목적 료해백세포개소(IL)-18재골관절염(OA)환자화비OA환자활막세포중표체적차이.방법 취OA환자(22례)여비OA환자(8례)관절활막조직,분별원대배양활막세포,채용매련면역흡부측정법(ELISA)정량검측2조활막세포배양상청액중IL-18단백적표체수평,채용면역세포화학방법 정성관찰IL-18재2조활막세포문표체적차이,통계학처리채용t검험.결과 ELISA검측OA환자세포배양상청액중IL-18적함량위(63.9±21.4)pg/ml, 비OA환자세포배양상청액중IL-18적함량위(17.7±1.5)pg/ml,2조비교차이유통계학의의(t=10.044,P<0.01);면역세포화학방법 현시재OA환자활막세포중IL-18단백적표체정양성.포질위종황색심염,이재비OA환자활막세포중포질담염.결론 IL-18재OA관절활막세포중표체상조, 제시IL-18가능직접삼여료OA적발병과정.
Objective To explore the pathological role of interleukin 18 (IL-18) in osteoarthritis (OA) by comparing the expression of IL-18 in the synoviocytes between OA patients and non-OA adults. Methods Synovium from OA patients (n=22) and non-OA adults were collected (n=8) and cultured. The concentrations of IL-18 were measured in the OA synoviocyte culture supernatant by enzyme-linked imm - unosorbent assay (ELISA) and were compared with non-OA adults. The expression of IL-18 was detected by cytochemical staining. Two-independence t-test was used for statistital analysis. Results The expression of IL-18 was higher in human OA synoviocyte culture supernatant when compared with that of the non-OA adults. The average concentration of IL-18 in the OA group was (63.9±21.4) pg/ml. The average concentration of IL-18 in the non-OA group was (17.7±1.5) pg/ml. There was statistical significant difference between them (P<0.05). IL-18 was stained deep brown in OA patients' synoviocytes while it was tint in non-OA adults' by cytochemical staining. Conclusion IL-18 is highly expressed in the synoviocytes of OA patients. It may be an important pro-inflammatory cytokine in the pathogenesis of OA.