CAJ | 학술논문

目的观察睾丸酮对MCF-7乳腺癌细胞株增殖、凋亡的影响,并探讨其机制.方法将1×10~(-5)、1×10~(-7)、1×10~(-9)、1×10~(-11) mol/L的睾丸酮分别作用于乳腺癌MCF-7细胞24、48、72 h,噻唑蓝(MTT)比色法检测细胞生长,流式细胞术检测不同浓度睾丸酮作用48 h时MCF-7细胞的周期分布和凋亡以及该细胞株中细胞周期素D1蛋白和雄激素受体的表达.结果高浓度睾丸酮抑制MCF-7乳腺癌细胞株的增殖,1×10~(-5) mol/L睾丸酮作用48 h时,细胞生长抑制率为22.21%,细胞凋亡率为(58.60±5.41)%,但可使细胞周期由G_1 期进入S期,并可使细胞周期素D1蛋白表达量增加,雄激素受体蛋白表达量下降.低浓度睾丸酮(1×10~(-9) mol/L)作用后细胞周期素D1蛋白表达量无明显变化,雄激素受体蛋白表达量升高,在短时间内(24 h)可促进MCF-7细胞增殖.结论高浓度睾丸酮在体外可使MCF-7乳腺癌细胞株细胞周期素D1蛋白表达增加,使细胞周期由G_1进入S期,而同时促使细胞凋亡,表现出抗肿瘤作用.低浓度睾丸酮有短暂的促进MCF-7乳腺癌细胞增殖的作用.
목적 관찰고환동대MCF-7유선암세포주증식、조망적영향,병탐토기궤제.방법 장1×10~(-5)、1×10~(-7)、1×10~(-9)、1×10~(-11) mol/L적고환동분별작용우유선암MCF-7세포24、48、72 h,새서람(MTT)비색법검측세포생장,류식세포술검측불동농도고환동작용48 h시MCF-7세포적주기분포화조망이급해세포주중세포주기소D1단백화웅격소수체적표체.결과 고농도고환동억제MCF-7유선암세포주적증식,1×10~(-5) mol/L고환동작용48 h시,세포생장억제솔위22.21%,세포조망솔위(58.60±5.41)%,단가사세포주기유G_1 기진입S기,병가사세포주기소D1단백표체량증가,웅격소수체단백표체량하강.저농도고환동(1×10~(-9) mol/L)작용후세포주기소D1단백표체량무명현변화,웅격소수체단백표체량승고,재단시간내(24 h)가촉진MCF-7세포증식.결론 고농도고환동재체외가사MCF-7유선암세포주세포주기소D1단백표체증가,사세포주기유G_1진입S기,이동시촉사세포조망,표현출항종류작용.저농도고환동유단잠적촉진MCF-7유선암세포증식적작용.
Objective To observe the effect of testosterone on the proliferation, apoptosis of MCF-7 breast cancer cells and investigate the mechanism. Methods MCF-7 cells were treated with testosterone at concentrations of 1×10~(-5),1×10~(-7),1 ×10~(-9),1×10~(-11) mol/L for 24,48 and 72 h respectively. Cell via-bility and growth inhibition rate of MCF-7 cells were assayed by MTr methods. Cell cycle, apoptosis rate and the expression of Cyclin D1, androgen receptor (AR) were detected by flow cytometry. Results High concentration testosterone could inhibit the proliferation of MCF-7 cells. After MCF-7 cells were treated with 1×10~(-5) mol/L testosterone for 48 h ,the cell growth inhibition rate was 22.21% ,and the apoptosis rate was (58.60±5.41 )%. High concentration testosterone could accelerate MCF-7 cell cycle progression from G_1 to S phases by increasing the expression of Cyclin D1 and decreasing the expression of AR. Low concentra-tion testosterone (1×10~(-9) mol/L) could increase the expression of AR, but had no effect on Cyclin D1, and it could promote MCF-7 cells proliferation in short time (24 h). Conclusion High concentration tes-tosterone can increase the expression of Cyclin D1 of MCF-7 cells and accelerate their cell cycle from G_1 to S phases,and then induce them to apoptosis in vitro. Low concentration testosterone can promote MCF-7 cells proliferation in short time.