CAJ | 학술논문

目的探讨细胞周期调控蛋白CDK4、p18、p19在食管鳞状细胞癌(SCC)发生、发展中的作用.方法制作组织芯片,用免疫组织化学EnVision二步法对120例食管癌患者手术标本中CDK4、p18、p19的表达进行检测,并对其结果进行统计分析.结果 CDK4蛋白在正常食管上皮的表达低[28.3%(34/120)],瘤变上皮中有所增高[32.5%(39/120)],食管SCC中表达高[84.2%(101/120)],且随SCC分化程度的降低而逐渐增高,SCC与正常上皮及瘤变上皮中CDK4表达阳性率差异有统计学意义(x2=76.004,P＜0.05;x2=65.897,P＜0.05).淋巴结转移组CDK4表达率[93.88%(46/49)]高于无淋巴结转移组[71.43%(55/71)](x 2=5.860,P＜0.05).p18、p19蛋白在正常食管上皮组织阳性表达率分别为34.2%(41/120)、29.2%(35/120),在食管瘤变上皮中表达率分别为19.2%(23/120)、15.0%(18/120),在SCC中表达率分别为63.3%(76/120)、61.7%(74/120),两指标在正常上皮及瘤变上皮间、瘤变上皮与SCC间、正常上皮与SCC间差异均有统计学意义(p18:x2=6.903、48.296、20.429,均P＜0.05;p19:x2=6.998、55.276、25.565,均P＜0.05);在食管SCC中随分化程度的降低而逐渐增高.p18、p19分别与CDK4基因表达呈正相关(r=0.696、0.630,均P＜0.05).p18与p19二者呈正相关(r=0.833,P＜0.05).结论细胞周期调控基因CDK4、p18、p19参与食管SCC的发生、发展,其蛋白表达与食管上皮癌变密切相关.
목적 탐토세포주기조공단백CDK4、p18、p19재식관린상세포암(SCC)발생、발전중적작용.방법 제작조직심편,용면역조직화학EnVision이보법대120례식관암환자수술표본중CDK4、p18、p19적표체진행검측,병대기결과진행통계분석.결과 CDK4단백재정상식관상피적표체저[28.3%(34/120)],류변상피중유소증고[32.5%(39/120)],식관SCC중표체고[84.2%(101/120)],차수SCC분화정도적강저이축점증고,SCC여정상상피급류변상피중CDK4표체양성솔차이유통계학의의(x2=76.004,P＜0.05;x2=65.897,P＜0.05).림파결전이조CDK4표체솔[93.88%(46/49)]고우무림파결전이조[71.43%(55/71)](x 2=5.860,P＜0.05).p18、p19단백재정상식관상피조직양성표체솔분별위34.2%(41/120)、29.2%(35/120),재식관류변상피중표체솔분별위19.2%(23/120)、15.0%(18/120),재SCC중표체솔분별위63.3%(76/120)、61.7%(74/120),량지표재정상상피급류변상피간、류변상피여SCC간、정상상피여SCC간차이균유통계학의의(p18:x2=6.903、48.296、20.429,균P＜0.05;p19:x2=6.998、55.276、25.565,균P＜0.05);재식관SCC중수분화정도적강저이축점증고.p18、p19분별여CDK4기인표체정정상관(r=0.696、0.630,균P＜0.05).p18여p19이자정정상관(r=0.833,P＜0.05).결론 세포주기조공기인CDK4、p18、p19삼여식관SCC적발생、발전,기단백표체여식관상피암변밀절상관.
Objective To investigate the role of cell cycle regulatory protein CDK4,p18,p19 in the genesis and development of esophageal squamous cell carcinoma (SCC).Methods Tissue microarray and immunohistochemical method (Envision) were used to detect the protein expression of CDK4,p18,p19 in 120 cases of esophageal tissues.The results were statistically analyzed.Results The positive rate of CDK4 protein expression in normal esophageal epithelium was low [28.3 % (34/120)],it increased in esophageal intraepithelial neoplasia [32.5 % (39/120)],and it was high in esophageal SCC [84.2 % (101/120)],which increased with the degree of SCC differentiation decreasing gradually.There was significant differences between the SCC and normal esophageal epithelium or esophageal intraepithelial neoplasia (x2= 76.004,P ＜0.05; x 2= 65.897,P ＜ 0.05).The expression of CDK4 in group with lymphatic metastasis [93.88 % (46/49)]was higher than without it [71.43 % (55/71)] (x2= 5.860,P ＜ 0.05).The positive rates of p18,p19 protein expression in normal esophageal epithelium were high [34.2 % (41/120),29.2 % (35/120)],it decreased in esophageal intraepithelial neoplasia [19.2 % (23/120),15.0 % (1 8/120)] (x 2= 134.481,P ＜ 0.05; x 2 = 141.376,P ＜ 0.05),but it were high in esophageal SCC [63.3 % (76/120) and 61.7 % (74/120)] which decreased with the degree of SCC differentiation gradually increased.There were significant differences between the normal esophageal epithelium and esophageal intraepithelial neoplasia,esophegeal intraepithelial neoplasia and SCC,normal esophageal epithelium and SCC (p 18:x 2 = 6.903,48.296,20.429,P ＜ 0.05; p1 9:x2 = 6.998,55.276,25.565,P＜ 0.05).CDK4 protein expression was correlated with both p18 and p19 (r =0.696,0.630,P ＜0.05),and there was significant positive correlation between the protein expression of p18 and p19 (r =0.833,P ＜0.05).Conclusion Cell cycle regulatory gene CDK4,p18,p19 get involved in the genesis and development of esophageal squamous cell carcinoma.Their protein expressions are closely related to canceration of esophageal epithelium.