生物化学与生物物理进展
生物化學與生物物理進展
생물화학여생물물리진전
PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
2006年
7期
673-676
,共4页
侯海峰%高增强%徐建华%许锐%李丽琴%李兰芬%梁宇和%苏晓东%刘鹏%冼鼎昌%董宇辉
侯海峰%高增彊%徐建華%許銳%李麗琴%李蘭芬%樑宇和%囌曉東%劉鵬%冼鼎昌%董宇輝
후해봉%고증강%서건화%허예%리려금%리란분%량우화%소효동%류붕%승정창%동우휘
脱氧胞嘧啶核苷酸脱氨酶%变性链球菌%变构调控%结晶
脫氧胞嘧啶覈苷痠脫氨酶%變性鏈毬菌%變構調控%結晶
탈양포밀정핵감산탈안매%변성련구균%변구조공%결정
deoxycytidylate deaminase%Streptococcus mutans%allosteric regulation%crystallization
脱氧胞嘧啶核苷酸脱氨酶属于脱氧胞苷酸脱氨家族.对来自于变性链球菌UA159的脱氧胞嘧啶核苷酸脱氨酶进行了克隆,在大肠杆菌中进行了表达,最后纯化.快速液相分子排阻色谱分析表明这种酶在溶液中形成六聚体.利用悬滴气相扩散技术获得了这个蛋白的晶体.在北京同步辐射的3W1A线站,收集了衍射分辨率到达3.1 A的数据.这个晶体属于P213空间群,其晶胞参数为a=b=c=113.2 A,α=β=γ=90°.计算可得马修斯系数为3.6A3·Da-1,据此可估计在一个不对称单位中含有两个单亚基.据目前所知,这是第一个关于野生型的脱氧胞嘧啶核苷酸脱氨酶的结晶学报道.
脫氧胞嘧啶覈苷痠脫氨酶屬于脫氧胞苷痠脫氨傢族.對來自于變性鏈毬菌UA159的脫氧胞嘧啶覈苷痠脫氨酶進行瞭剋隆,在大腸桿菌中進行瞭錶達,最後純化.快速液相分子排阻色譜分析錶明這種酶在溶液中形成六聚體.利用懸滴氣相擴散技術穫得瞭這箇蛋白的晶體.在北京同步輻射的3W1A線站,收集瞭衍射分辨率到達3.1 A的數據.這箇晶體屬于P213空間群,其晶胞參數為a=b=c=113.2 A,α=β=γ=90°.計算可得馬脩斯繫數為3.6A3·Da-1,據此可估計在一箇不對稱單位中含有兩箇單亞基.據目前所知,這是第一箇關于野生型的脫氧胞嘧啶覈苷痠脫氨酶的結晶學報道.
탈양포밀정핵감산탈안매속우탈양포감산탈안가족.대래자우변성련구균UA159적탈양포밀정핵감산탈안매진행료극륭,재대장간균중진행료표체,최후순화.쾌속액상분자배조색보분석표명저충매재용액중형성륙취체.이용현적기상확산기술획득료저개단백적정체.재북경동보복사적3W1A선참,수집료연사분변솔도체3.1 A적수거.저개정체속우P213공간군,기정포삼수위a=b=c=113.2 A,α=β=γ=90°.계산가득마수사계수위3.6A3·Da-1,거차가고계재일개불대칭단위중함유량개단아기.거목전소지,저시제일개관우야생형적탈양포밀정핵감산탈안매적결정학보도.
Deoxycytidylate (dCMP) deaminase is an enzyme belonged to dCMP cyt deam family. The dCMP deaminase from Streptococcus mutans UA159 was cloned and expressed in E. coli, and purified to homogeneity. The FPLC size exclusion chromatography analysis reveals that the S. mutans dCMP deaminase forms hexamer in solution. The protein was crystallized using hanging drop vapour-diffusion method and diffracted to a resolution of 3.1 (A). The diffraction data were collected at BSRF beamline3W1A. The crystals belong to P213 space group, with unit cell parameters a = b = c = 113.2(A), α =β = γ = 90°. Assuming there are two subunits per asymmetric unit, the Matthews coefficient is 3.6 (A)3 ·Da-1. This is the first crystallization report of the wild-type deoxycytidylate deaminase.
