CAJ | 학술논문

目的探讨维生素C、E(VC、VE)干预对氟中毒大鼠肝、肾和脑组织超微结构变化的影响.方法将120只Wistar大鼠分为9组.对照组饮用纯净水;染氟组饮用高氟水(含氟化钠150 mg/L).染氟同时.VC干预组分别用50、100、150 mg·kg-1·d-13种剂量的VC灌胃;VE干预组分别用25、50、75 mg·kg-1·d-1 3种剂量的VE灌胃;VC和VE联合干预组以100 mg·kg-1·d-1VC和50 mg·kg-1·d-1VE同时灌胃.9个月后处死大鼠,用透射电子显微镜观察各组大鼠肝、肾、脑组织超微结构变化.结果大鼠饮用高氟水后.肝、肾、脑组织发生了不同程度的超微结构病理变化.①肝细胞水肿,线粒体基质变淡,在肝细胞内可见脂滴,核基质淡,核仁边集.肝血窦内皮明显肿胀; ②肾小管细胞核异染色质较多且边集,细胞间隙和质膜内褶增宽,内质网扩张,集合管上皮细胞内的亮细胞胞质极度松散;③脑胶质细胞肿胀,细胞器很少,细胞核异染色质较多、边集.VC、VE各干预组肝、肾、脑组织超微结构变化轻微或无明显变化,其中VC、VE联合干预组各组织超微结构基本与对照组相同.结论氟中毒可引起大鼠肝、肾、脑组织损害.VC、VE单独及联合干预均对氟中毒引起的组织器官损害有一定的保护作用,联合干预效果强于单独干预.
목적 탐토유생소C、E(VC、VE)간예대불중독대서간、신화뇌조직초미결구변화적영향.방법 장120지Wistar대서분위9조.대조조음용순정수;염불조음용고불수(함불화납150 mg/L).염불동시.VC간예조분별용50、100、150 mg·kg-1·d-13충제량적VC관위;VE간예조분별용25、50、75 mg·kg-1·d-1 3충제량적VE관위;VC화VE연합간예조이100 mg·kg-1·d-1VC화50 mg·kg-1·d-1VE동시관위.9개월후처사대서,용투사전자현미경관찰각조대서간、신、뇌조직초미결구변화.결과 대서음용고불수후.간、신、뇌조직발생료불동정도적초미결구병리변화.①간세포수종,선립체기질변담,재간세포내가견지적,핵기질담,핵인변집.간혈두내피명현종창; ②신소관세포핵이염색질교다차변집,세포간극화질막내습증관,내질망확장,집합관상피세포내적량세포포질겁도송산;③뇌효질세포종창,세포기흔소,세포핵이염색질교다、변집.VC、VE각간예조간、신、뇌조직초미결구변화경미혹무명현변화,기중VC、VE연합간예조각조직초미결구기본여대조조상동.결론 불중독가인기대서간、신、뇌조직손해.VC、VE단독급연합간예균대불중독인기적조직기관손해유일정적보호작용,연합간예효과강우단독간예.
Objective To observe the effects of vitamin C (VC) and E (VE) on the uhrastrueture of liver, kidney and brain tissue of fluorosis rats. Methods One hundred and twenty Wistar rats were chosen as the experimental animals and were divided into 9 groups randomly. The control group were given distilled water and the fluoride exposed group were given distilled water containing sodium fluoride 150 mg/L. The throe VC-fluoride exposed groups were given VC orally in a dose of 50,100,150 mg·kg-1.d-1, respectively, and the three VE-fluoride exposed groups were given VE of 25,50,75 mg·kg-1·d-1, respectively. The VC-VE-fluoride exposed group were given VC of 100 mg·kg-1·d-1and VE of 50 mg·kg-1·d-1at the same time of high fluoride water intake. The rats were sacrificed after 9 months and the ultrastructure changes on liver, kidney and brain tissues of each group were observed under transmission electron microscope(TEM). Results The uhrastrueture of liver, kidney and brain showed pathologic changes in the rats that drank water containing high eoneentrations of sodium fluoride. ①Edema of hepatocytes, smeared mitochontria and nuclear matrix, lipid droplet in eytoplasm of hepatocytes, margination of nueleohs as well as obvious swelling of liver sinusoidal endothelial were observed in fluoride exposed group. ② There were marginafion of heterochromatin, expansion of cell space and endoplasmic reticulum in the kidney after the exposure to excess fluoride.③Signifieant changes were found on glial eells on the brain, including cell swelling, increase and marginafion of heterochromatin in the fluoride exposure group. There were no significant uhrastrueture changes in the VC or VE intervention group, while the VC-VE-fluoride exposure group was almost the same as the control group. Conclusions Fluoresis may cause damage on liver, kidney and brain in rats. VC and VE, alone or combined, have protective effects, and the combined supplementation was stronger than single supplementations.