中华妇产科杂志
中華婦產科雜誌
중화부산과잡지
CHINESE JOUNAL OF OBSTETRICS AND GYNECOLOGY
2008年
3期
213-217
,共5页
于璇%邓晓惠%晁岚%于红玲%刘文君
于璇%鄧曉惠%晁嵐%于紅玲%劉文君
우선%산효혜%조람%우홍령%류문군
卵巢%移植%自体%低温保存%促性腺素类%小鼠
卵巢%移植%自體%低溫保存%促性腺素類%小鼠
란소%이식%자체%저온보존%촉성선소류%소서
Ovary%Transplantation,autologous%Cryopreservation%Gonadotropins%Mice
目的 探讨冻融后移植的小鼠卵巢组织对促性腺激素的反应.方法 将36只性成熟雌性小白鼠随机分为新鲜移植组、冻融移植组和对照组,每组12只.新鲜移植组小鼠切除双侧卵巢,将卵巢切成小组织块,立即移植人双侧肾被膜下;冻融移植组小鼠切除双侧卵巢,将卵巢切成小组织块,采用玻璃化冷冻方法冷冻保存,2周后将冷冻卵巢组织复苏,移植入小鼠双侧肾被膜下.卵巢组织移植2周后,新鲜移植组和冻融移植组每组随机取6只小鼠应用7.5 IU人绝经期促性腺激素及10 IU绒毛膜促性腺激素,观察移植后的卵巢组织对促性腺激素的反应.同时应用免疫组化染色方法观察各组卵泡中卵泡刺激素受体的表达情况.结果 新鲜移植组、冻融移植组和对照组未应用促性腺激素小鼠卵巢组织内近成熟卵泡百分率分别为2.3%、2.3%和2.6%,应用促性腺激素小鼠卵巢组织内近成熟卵泡百分率分别为4.2%、4.0%和5.8%,各组内分别比较,差异均有统计学意义(P＜0.05);新鲜移植组和冻融移植组与对照组比较,差异均元统计学意义(P＞0.05).新鲜移植组、冻融移植组和对照组卵泡刺激素受体表达积分吸光度值在窦状卵泡中分别为9408±2777、9175±3093和8838±2064,在窦前卵泡中分别为4531±1903、4808±1386和5516±1136,各组间分别比较,差异均无统计学意义(P＞0.05).结论 卵巢组织冷冻保存、复苏及移植过程未影响卵巢卵泡刺激素受体的表达,冻融后移植的小鼠卵巢组织对外源性促性腺激素的反应未受冷冻、复苏及移植等过程影响.
目的 探討凍融後移植的小鼠卵巢組織對促性腺激素的反應.方法 將36隻性成熟雌性小白鼠隨機分為新鮮移植組、凍融移植組和對照組,每組12隻.新鮮移植組小鼠切除雙側卵巢,將卵巢切成小組織塊,立即移植人雙側腎被膜下;凍融移植組小鼠切除雙側卵巢,將卵巢切成小組織塊,採用玻璃化冷凍方法冷凍保存,2週後將冷凍卵巢組織複囌,移植入小鼠雙側腎被膜下.卵巢組織移植2週後,新鮮移植組和凍融移植組每組隨機取6隻小鼠應用7.5 IU人絕經期促性腺激素及10 IU絨毛膜促性腺激素,觀察移植後的卵巢組織對促性腺激素的反應.同時應用免疫組化染色方法觀察各組卵泡中卵泡刺激素受體的錶達情況.結果 新鮮移植組、凍融移植組和對照組未應用促性腺激素小鼠卵巢組織內近成熟卵泡百分率分彆為2.3%、2.3%和2.6%,應用促性腺激素小鼠卵巢組織內近成熟卵泡百分率分彆為4.2%、4.0%和5.8%,各組內分彆比較,差異均有統計學意義(P＜0.05);新鮮移植組和凍融移植組與對照組比較,差異均元統計學意義(P＞0.05).新鮮移植組、凍融移植組和對照組卵泡刺激素受體錶達積分吸光度值在竇狀卵泡中分彆為9408±2777、9175±3093和8838±2064,在竇前卵泡中分彆為4531±1903、4808±1386和5516±1136,各組間分彆比較,差異均無統計學意義(P＞0.05).結論 卵巢組織冷凍保存、複囌及移植過程未影響卵巢卵泡刺激素受體的錶達,凍融後移植的小鼠卵巢組織對外源性促性腺激素的反應未受冷凍、複囌及移植等過程影響.
목적 탐토동융후이식적소서란소조직대촉성선격소적반응.방법 장36지성성숙자성소백서수궤분위신선이식조、동융이식조화대조조,매조12지.신선이식조소서절제쌍측란소,장란소절성소조직괴,립즉이식인쌍측신피막하;동융이식조소서절제쌍측란소,장란소절성소조직괴,채용파리화냉동방법냉동보존,2주후장냉동란소조직복소,이식입소서쌍측신피막하.란소조직이식2주후,신선이식조화동융이식조매조수궤취6지소서응용7.5 IU인절경기촉성선격소급10 IU융모막촉성선격소,관찰이식후적란소조직대촉성선격소적반응.동시응용면역조화염색방법관찰각조란포중란포자격소수체적표체정황.결과 신선이식조、동융이식조화대조조미응용촉성선격소소서란소조직내근성숙란포백분솔분별위2.3%、2.3%화2.6%,응용촉성선격소소서란소조직내근성숙란포백분솔분별위4.2%、4.0%화5.8%,각조내분별비교,차이균유통계학의의(P＜0.05);신선이식조화동융이식조여대조조비교,차이균원통계학의의(P＞0.05).신선이식조、동융이식조화대조조란포자격소수체표체적분흡광도치재두상란포중분별위9408±2777、9175±3093화8838±2064,재두전란포중분별위4531±1903、4808±1386화5516±1136,각조간분별비교,차이균무통계학의의(P＞0.05).결론 란소조직냉동보존、복소급이식과정미영향란소란포자격소수체적표체,동융후이식적소서란소조직대외원성촉성선격소적반응미수냉동、복소급이식등과정영향.
Objective To investigate the response of cryopreserved ovarian tissue after autologous implantation in mouse stimulated with gonadotrophin.Methods Thirty six female mice were randomly divided into three groups,with 12 mice in each group.In group of fresh ovarian tissue,fresh ovarian tissue was implanted into kidney capsule of mice:in group of cryopreserved ovarian tissue,ovarian tissue was implanted into kidney capsule of mice after cryopreserved by vitrification for two weeks.We investigated the response of ovarian tissue two weeks later after autologous implantation stimulated with gonadotrophin.Immunohistochemistry staining method was used to observe the expression of follicle stimulating hormone receptor.Results Before and after stimularian with gonadotrophin,the mature follicle rate of group of fresh ovarian tissue was 2.3%and 4.2%.that of group of cryopreserved ovarian tissue was 2.3%and 4.0%,and that of group of control was 2.6%and 5.8%.Regarding the percentages of mature follicle.there were significant differences after stimulation with gonadotrophin(P＜0.05).After stimulating with gonadotrophin the percentages of mature follicle were the same in the fresh tissue group,cryopreserved tissue group and control group(P＞0.05).The integrated optical density of follicle stimulating hormone receptor of fresh ovarian tissue in antrofollicle and pre-antrofollicle were 9408±2777 and 4531±1903.that of cryopreserved ovarian tissue were 9175±3093 and 4808±1386.and that of the control ovarian tissue were 8838±2064and 5516±1136 respectively.There was no significant difference between any two groups(P＞0.05).Conclusion The follicle stimulating hormone receptor is preserved by cryopreservation and transplantation,small pieces of ovarian tissue response to gonadotropin stimulation is normal.
