中国行为医学科学
中國行為醫學科學
중국행위의학과학
2008年
5期
397-399
,共3页
房恒通%蒋与刚%房红芸%刘静%庞伟
房恆通%蔣與剛%房紅蕓%劉靜%龐偉
방항통%장여강%방홍예%류정%방위
学习记忆%银杏叶提取物%蓝莓提取物%复合营养素
學習記憶%銀杏葉提取物%藍莓提取物%複閤營養素
학습기억%은행협제취물%람매제취물%복합영양소
Learning and memory%Ginkgo biloba%Blue berry%Combined nutrients
目的 观察银杏叶、蓝莓提取物与复合营养素对老龄大鼠学习记忆功能及抗氧化能力的影响.方法 15月龄Wistar大鼠20只,随机分为对照组和干预组,每组10只,雌雄各半.对照组和干预组大鼠分别饲喂基础饲料及添加复合营养素的自制饲料,同时灌胃蒸馏水或植物提取物混合液.灌胃剂量为4ml·kg-1·d-1,实验期10周,干预结束后采用水迷宫实验检测大鼠的学习记忆功能.然后处死动物.荧光法测定血清维生素E含量;紫外可见分光光度法测定血清总抗氧化能力(T-AOC)、抑制羟自由基能力;全血谷胱甘肽过氧化物酶(GSH-Px)活性采用DTNB法测定.结果 干预组老龄大鼠水迷宫实验训练期及检测期逃避潜伏期[分别为(7.74±1.84)s,(6.85±0.82)s]均低于对照组[分别为(10.14±2.05)s,(9.92±2.30)s],差异具有显著性(t=4.506,t=4.300,P＜0.05).干预组老龄大鼠血清VitE含量[(16.22 ±5.03)μg/ml]高于对照组[(7.52±4.24)μg/ml],差异具有显著性(t=6.058,P＜0.05).干预组老龄大鼠血清T-AOC、GSH-Px活性[分别为(13.01±9.36)U/ml,(47.27±8.60)活力单位]高于对照组[(6.51±1.39)U/ml,(33.48 ±3.19)活力单位],差异具有显著性(t=4.113,t=7.679,P＜0.05).与对照组比较,干预组老龄大鼠的学习记忆功能得到改善;血清维生素E含量增加;T-AOC、抑制羟自由基能力提高;GSH-Px活性升高.结论 银杏叶、蓝莓提取物与复合营养素对老龄大鼠的学习记忆功能有改善作用,其作用途径可能与提高抗氧化防御系统功能有关.
目的 觀察銀杏葉、藍莓提取物與複閤營養素對老齡大鼠學習記憶功能及抗氧化能力的影響.方法 15月齡Wistar大鼠20隻,隨機分為對照組和榦預組,每組10隻,雌雄各半.對照組和榦預組大鼠分彆飼餵基礎飼料及添加複閤營養素的自製飼料,同時灌胃蒸餾水或植物提取物混閤液.灌胃劑量為4ml·kg-1·d-1,實驗期10週,榦預結束後採用水迷宮實驗檢測大鼠的學習記憶功能.然後處死動物.熒光法測定血清維生素E含量;紫外可見分光光度法測定血清總抗氧化能力(T-AOC)、抑製羥自由基能力;全血穀胱甘肽過氧化物酶(GSH-Px)活性採用DTNB法測定.結果 榦預組老齡大鼠水迷宮實驗訓練期及檢測期逃避潛伏期[分彆為(7.74±1.84)s,(6.85±0.82)s]均低于對照組[分彆為(10.14±2.05)s,(9.92±2.30)s],差異具有顯著性(t=4.506,t=4.300,P＜0.05).榦預組老齡大鼠血清VitE含量[(16.22 ±5.03)μg/ml]高于對照組[(7.52±4.24)μg/ml],差異具有顯著性(t=6.058,P＜0.05).榦預組老齡大鼠血清T-AOC、GSH-Px活性[分彆為(13.01±9.36)U/ml,(47.27±8.60)活力單位]高于對照組[(6.51±1.39)U/ml,(33.48 ±3.19)活力單位],差異具有顯著性(t=4.113,t=7.679,P＜0.05).與對照組比較,榦預組老齡大鼠的學習記憶功能得到改善;血清維生素E含量增加;T-AOC、抑製羥自由基能力提高;GSH-Px活性升高.結論 銀杏葉、藍莓提取物與複閤營養素對老齡大鼠的學習記憶功能有改善作用,其作用途徑可能與提高抗氧化防禦繫統功能有關.
목적 관찰은행협、람매제취물여복합영양소대노령대서학습기억공능급항양화능력적영향.방법 15월령Wistar대서20지,수궤분위대조조화간예조,매조10지,자웅각반.대조조화간예조대서분별사위기출사료급첨가복합영양소적자제사료,동시관위증류수혹식물제취물혼합액.관위제량위4ml·kg-1·d-1,실험기10주,간예결속후채용수미궁실험검측대서적학습기억공능.연후처사동물.형광법측정혈청유생소E함량;자외가견분광광도법측정혈청총항양화능력(T-AOC)、억제간자유기능력;전혈곡광감태과양화물매(GSH-Px)활성채용DTNB법측정.결과 간예조노령대서수미궁실험훈련기급검측기도피잠복기[분별위(7.74±1.84)s,(6.85±0.82)s]균저우대조조[분별위(10.14±2.05)s,(9.92±2.30)s],차이구유현저성(t=4.506,t=4.300,P＜0.05).간예조노령대서혈청VitE함량[(16.22 ±5.03)μg/ml]고우대조조[(7.52±4.24)μg/ml],차이구유현저성(t=6.058,P＜0.05).간예조노령대서혈청T-AOC、GSH-Px활성[분별위(13.01±9.36)U/ml,(47.27±8.60)활력단위]고우대조조[(6.51±1.39)U/ml,(33.48 ±3.19)활력단위],차이구유현저성(t=4.113,t=7.679,P＜0.05).여대조조비교,간예조노령대서적학습기억공능득도개선;혈청유생소E함량증가;T-AOC、억제간자유기능력제고;GSH-Px활성승고.결론 은행협、람매제취물여복합영양소대노령대서적학습기억공능유개선작용,기작용도경가능여제고항양화방어계통공능유관.
Objective To investigate the effects of ginkgo biloba and blue berry extract and combined nutrients on learning and memory function and anti-oxidation capability in aged rats.Methods Twenty Wistar rats (15 months old)were randomly divided into intervened group and control group,five male and five female in each.The rats in intervened group were fed with diets supplemented with combined nutrients and mixed plant extract adThe experiment lasted 10 weeks.The learning and memory function of aged rats were tested with water maze.The level of vitamin E in serum was determined by fluorospectrophotometry,serum total anti-oxidation capability,hydroxy radical inhibition capability by UV-Vis Spectrophotometry.The activity of glutathione peroxidase was determined by dithio-nitrobenzene method.Results The escaping latency of intervened group in testing and training period[(7.74±1.84)s,(6.85±0.82)s]was less than that of control group[(10.14±2.05)s,(9.92±2.30)s]and the differences were significant(t=4.506,t=4.300,P＜0.05).The concentration of serum vitamin E in intervened group[(16.22±5.03)μg/ml]was higher than that of control group[(7.52±4.24)μg/ml]and the difference was significant(t=6.058,P＜0.05).The total anti-oxidation capability and activity of glutathione peroxidase in serum in intervened group[(13.01±9.36)U/ml,(47.27 ±8.60)activity unit]were higher than those of control group[(6.51±1.39)U/ml,(33.48±3.19)activity unit]and the difference were significant(t=4.113,t=7.679,P＜0.05).Compared with control group,the learning and memory function of aged rats in intervened group was improved,the vitamin E contents in serum,the total anti-oxidation capability,hydroxy radical inhibition capability and the activity of glutathione peroxidase were increased.Conclusion Ginkgo biloba and blue berry extract and combined nutrients have improved effect on learning and memory function of aged rats and the effect might correlate with improved anti-oxidation status.
