CAJ | 학술논문

目的观察3-甲基腺嘌呤(3-methlyadenine,3-MA)对大鼠骨髓源性内皮祖细胞(endothelial progenitor cells,EPCs)增殖、凋亡及细胞周期的影响.方法密度梯度法取大鼠骨髓EPCs,体外诱导分化并鉴定.实验分对照及不同浓度(1.25、2.5、5、10 mmol/L)3-MA组.四氮唑蓝(methyl thiazolyl tetrazolium,MTT)法测细胞增殖,流式细胞仪测凋亡率及细胞周期.结果 (1)5 mmol/L组促EPCs增殖;10 mmol/L组抑制EPCs增殖,差异有统计学意义(P＜0.05).其余两组对EPCs增殖无明显影响,差异无统计学意义(P＞0.05).(2)10 mmol/L组促EPCs凋亡,差异有统计学意义(P＜0.05).其余各组对EPCs凋亡率无明显影响,与对照组比较差异无统计学意义(P＞0.05).(3)5、10 mmol/L组影响细胞周期,5 mmoL/L组G0/G1期细胞减少、S和G2/M期增加;10 mmol/L组发生S期阻滞,G2/M细胞减少;差异有统计学意义(P＜0.05).结论 3-MA对EPCs增殖及凋亡的影响与其浓度有关,当浓度为10 mmol/L时抑制增殖并促进凋亡.
목적 관찰3-갑기선표령(3-methlyadenine,3-MA)대대서골수원성내피조세포(endothelial progenitor cells,EPCs)증식、조망급세포주기적영향.방법 밀도제도법취대서골수EPCs,체외유도분화병감정.실험분대조급불동농도(1.25、2.5、5、10 mmol/L)3-MA조.사담서람(methyl thiazolyl tetrazolium,MTT)법측세포증식,류식세포의측조망솔급세포주기.결과 (1)5 mmol/L조촉EPCs증식;10 mmol/L조억제EPCs증식,차이유통계학의의(P＜0.05).기여량조대EPCs증식무명현영향,차이무통계학의의(P＞0.05).(2)10 mmol/L조촉EPCs조망,차이유통계학의의(P＜0.05).기여각조대EPCs조망솔무명현영향,여대조조비교차이무통계학의의(P＞0.05).(3)5、10 mmol/L조영향세포주기,5 mmoL/L조G0/G1기세포감소、S화G2/M기증가;10 mmol/L조발생S기조체,G2/M세포감소;차이유통계학의의(P＜0.05).결론 3-MA대EPCs증식급조망적영향여기농도유관,당농도위10 mmol/L시억제증식병촉진조망.
Objective To investigate the effect of proliferation,apoptosis and cell cycle of 3-MA on rat endothelial progenitor cells. Methods Bone marrow-derived mononuclear cells were isolated from rat bone marrow by ficoll. There were five groups. The control group and four 3-MA concentration groups: 1. 25 mmol/L,2. 5 mmol/L,5 mmol/L, 10 mmol/L. MTT was used to measure the proliferation of endothelial progenitor cells. Flow cytometry ( FCM) was used to detect cell apoptosis and cell cycle. Results (1)5 mmol/L 3-MA promotes proliferation of endothelial progenitor cells, while 10 mmol/L 3-MA inhibits the proliferation of endothelial progenitor cells (P ＜ 0. 05). (2) 10 mmol/L 3-MA promotes apoptosis of endothelial progenitor cells, compared with the control, the difference was significant ( P ＜ 0. 05 ). (3) 3-MA at the concentration of 5 mmol/L reduces cells at G0/G1 phase and increases S and G2/M phase cells; 10 mmol/L 3-MA induces endothelial progenitor cells blockade at S phase, G2/M phase cells decreased, compared with the control, the difference was significant (P ＜ 0. 05). Conclusions 5 mmol/L 3-MA promotes the proliferation of endothelial progenitor cells. 10 mmol/L 3-MA inhibits the proliferation and promotes apoptosis of endothelial progenitor cells.