中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2012年
8期
711-715
,共5页
表柔比星%细胞增殖%肿瘤浸润%细胞黏附%胃肿瘤
錶柔比星%細胞增殖%腫瘤浸潤%細胞黏附%胃腫瘤
표유비성%세포증식%종류침윤%세포점부%위종류
Epirubicin%Cell proliferation%Neoplasm invasveness%Cell adhesion%Stomach neoplasms
目的 探讨唑来膦酸(ZOL)联合阿霉素(ADM)对胃癌SGC-7901细胞株增殖、侵袭和黏附的影响.方法 采用MTT法检测不同浓度的唑来膦酸和阿霉素对胃癌细胞株SGC-7901的增殖和细胞黏附作用;Transwell实验分析药物对细胞侵袭能力的影响;Real-time PCR检测药物对细胞株MMP-2、TIMP-2、MMP-9、ICAM 1和CD44 mRNA表达的影响.结果 唑来膦酸浓度10-6、10-5、10-4、10-3 mol/L作用于SGC-7901细胞48 h后,细胞生长抑制率分别为4.98％、12.19％、27.34％、73.13％,与对照组比较,抑制率均显著升高(P＜0.01),且抑制作用随着剂量的升高和时间的延长而增强(P＜0.05);10-4 mol/l浓度的唑来膦酸和0.5 mg/L浓度的阿霉素单独以及联合用药作用于细胞,观察2h、4h、6h后,黏附率分别为:5.03％、24.38％、59.65％;4.40％、19.77％、51.22％;4.28％、18.62％、51.02％; 4.02％、15.02％、46.95％,与对照组比较,加入药物后,细胞的黏附能力有不同程度的降低(P＜0.05),且呈时间依赖性,但联合用药组与相应浓度单药组比较差异无统计学意义(P＞0.05);上述浓度的药物作用于细胞,每个高倍视野细胞数(个)分别为:105.11±10.43、98.37±15.75、96.19±9.24、83.02±19.72,与对照组比较,单药有减弱侵袭力的趋势,但作用较弱(P＞0.05),联合用药时,细胞的侵袭能力则明显下降(P＜0.05);唑来膦酸和阿霉素单药均有降低CD44、MMP-2、MMP-9、ICAM-1 mRNA和升高TIPM-2 mRNA表达水平的作用,联合用药后该作用则表现更为显著(P＜0.05或P＜0.01).结论 唑来膦酸和阿霉素对人胃癌细胞株SGC-7901具有一定的抑制增殖、降低细胞黏附和侵袭能力的作用,且两药联合具有协同抗肿瘤作用,可能和下调ICAM-1、MMP 2、MMP-9、CD44 mRNA的表达以及上调TIMP-2 mRNA的表达水平相关.
目的 探討唑來膦痠(ZOL)聯閤阿黴素(ADM)對胃癌SGC-7901細胞株增殖、侵襲和黏附的影響.方法 採用MTT法檢測不同濃度的唑來膦痠和阿黴素對胃癌細胞株SGC-7901的增殖和細胞黏附作用;Transwell實驗分析藥物對細胞侵襲能力的影響;Real-time PCR檢測藥物對細胞株MMP-2、TIMP-2、MMP-9、ICAM 1和CD44 mRNA錶達的影響.結果 唑來膦痠濃度10-6、10-5、10-4、10-3 mol/L作用于SGC-7901細胞48 h後,細胞生長抑製率分彆為4.98％、12.19％、27.34％、73.13％,與對照組比較,抑製率均顯著升高(P＜0.01),且抑製作用隨著劑量的升高和時間的延長而增彊(P＜0.05);10-4 mol/l濃度的唑來膦痠和0.5 mg/L濃度的阿黴素單獨以及聯閤用藥作用于細胞,觀察2h、4h、6h後,黏附率分彆為:5.03％、24.38％、59.65％;4.40％、19.77％、51.22％;4.28％、18.62％、51.02％; 4.02％、15.02％、46.95％,與對照組比較,加入藥物後,細胞的黏附能力有不同程度的降低(P＜0.05),且呈時間依賴性,但聯閤用藥組與相應濃度單藥組比較差異無統計學意義(P＞0.05);上述濃度的藥物作用于細胞,每箇高倍視野細胞數(箇)分彆為:105.11±10.43、98.37±15.75、96.19±9.24、83.02±19.72,與對照組比較,單藥有減弱侵襲力的趨勢,但作用較弱(P＞0.05),聯閤用藥時,細胞的侵襲能力則明顯下降(P＜0.05);唑來膦痠和阿黴素單藥均有降低CD44、MMP-2、MMP-9、ICAM-1 mRNA和升高TIPM-2 mRNA錶達水平的作用,聯閤用藥後該作用則錶現更為顯著(P＜0.05或P＜0.01).結論 唑來膦痠和阿黴素對人胃癌細胞株SGC-7901具有一定的抑製增殖、降低細胞黏附和侵襲能力的作用,且兩藥聯閤具有協同抗腫瘤作用,可能和下調ICAM-1、MMP 2、MMP-9、CD44 mRNA的錶達以及上調TIMP-2 mRNA的錶達水平相關.
목적 탐토서래련산(ZOL)연합아매소(ADM)대위암SGC-7901세포주증식、침습화점부적영향.방법 채용MTT법검측불동농도적서래련산화아매소대위암세포주SGC-7901적증식화세포점부작용;Transwell실험분석약물대세포침습능력적영향;Real-time PCR검측약물대세포주MMP-2、TIMP-2、MMP-9、ICAM 1화CD44 mRNA표체적영향.결과 서래련산농도10-6、10-5、10-4、10-3 mol/L작용우SGC-7901세포48 h후,세포생장억제솔분별위4.98％、12.19％、27.34％、73.13％,여대조조비교,억제솔균현저승고(P＜0.01),차억제작용수착제량적승고화시간적연장이증강(P＜0.05);10-4 mol/l농도적서래련산화0.5 mg/L농도적아매소단독이급연합용약작용우세포,관찰2h、4h、6h후,점부솔분별위:5.03％、24.38％、59.65％;4.40％、19.77％、51.22％;4.28％、18.62％、51.02％; 4.02％、15.02％、46.95％,여대조조비교,가입약물후,세포적점부능력유불동정도적강저(P＜0.05),차정시간의뢰성,단연합용약조여상응농도단약조비교차이무통계학의의(P＞0.05);상술농도적약물작용우세포,매개고배시야세포수(개)분별위:105.11±10.43、98.37±15.75、96.19±9.24、83.02±19.72,여대조조비교,단약유감약침습력적추세,단작용교약(P＞0.05),연합용약시,세포적침습능력칙명현하강(P＜0.05);서래련산화아매소단약균유강저CD44、MMP-2、MMP-9、ICAM-1 mRNA화승고TIPM-2 mRNA표체수평적작용,연합용약후해작용칙표현경위현저(P＜0.05혹P＜0.01).결론 서래련산화아매소대인위암세포주SGC-7901구유일정적억제증식、강저세포점부화침습능력적작용,차량약연합구유협동항종류작용,가능화하조ICAM-1、MMP 2、MMP-9、CD44 mRNA적표체이급상조TIMP-2 mRNA적표체수평상관.
Objective To explore the possible effect of combined treatment of zoledronic acid and doxorubicin on proliferation,invasion and adhesion of SGC7901 cell line,and potential underlying mechanisms. Methods MTT and Transwell experiments were used respectively to investigate the effect of different concentrations of zoledronic acid and doxorubicin on the proliferation,adhesion and invasion of SGC7901. The mRNA levels of MMP2, TIMP2, MMP9, ICAM1 and CD44 were quantified by real time PCR. Results Gastric cell line SGC-7901 were incubated with zoledronic acid for 48 h in different concentration of 10-6,10-5,10-4,10-3 mol/L,their inhibition rates were 4.98％,12.19％,27.34％ and 73.13％ separately,which were higher than control group (P＜0.01),and enhanced along with the dose increasing and the time extension (P＜0.05).Gastric cell line SGC-7901 was incubated with zoledronic acid 10-4 mol /L and doxorubicin 0.5 mg/L,respectively,or in combination for 2 h,4 h,6 h,and thereafter,the detected adhesion rates were 5.03％,24.38％ and 59.65％ in control group,4.40％,19.77％ and 51.22％ in zoledronic acid group,4.28％, 18.62％ and 51.02％ in doxorubicin group,4.02 ％,15.02％ and 46.95％ in combination group. The adhesion rates were reduced after incubation with different medicament concentration (P＜0.05).In the study of cell invasion,the cell numbers per high power field were:105.11 ± 10.43,98.37 ± 15.75,96.19 ± 9.24,83.02 ± 19.72 after treatment with zoledronic acid,doxorubicin or their combination at different concentrations. Compared with control, signal medicament had weak effectiveness in invasiveness in SGC-7901 (P＞ 0.05),whereas in combination group it was significantly decreased (P＜0.05).Both zoledronic acid and doxorubicin decreased the mRNA expressions of CD44,MMP2,MMP9,ICAM1 while increased TIMP2 mRNA level (P＜0.05or P＜0.01) versus control.The effect was more evident with combination treatment(P＜0.05 or P＜0.01),compared with doxorubicin group. Conclusions Both zoledronic acid and doxorubicin can inhibit the proliferation,adhesion and invasion of SGC7901. Combination treatment has additive effect.It may be related to the down-regulation of mRNA of ICAM1,MMP2,MMP9,CD44 and up-regulation of mRNA of TIMP2.
