CAJ | 학술논문

目的观察缺氧诱导因子-1α(HIF-1α)、基质金属蛋白酶-9(MMP-9)和转化生长因子(TGF)-β1在大鼠肝纤维化组织中的表达以及TGF-β1疫苗对这些因子表达的影响.方法二甲基亚硝胺建立大鼠肝纤维化模型,并予以TGF-β1疫苗干预.在实验6周末用免疫组织化学方法检测HIF-1α、MMP-9和TGF-β1在大鼠肝纤维化组织中的表达情况.组间比较采用单因素方差分析.方差齐性者两两比较采用LSD法,方差不齐者进行Dunnet's T3检验.结果与对照组比较,肝纤维化模型组及TGF-β1疫苗干预组HIF-1α、MMP-9、TGF-β1表达显著增加(P＜0.01),且与肝纤维化程度呈正相关关系(r值分别为0.911、0.814、0.836,P＜0.01);与肝纤维化模型组比较,TGF-β1疫苗干预组HIF-1α、MMP-9和TGF-β1表达显著降低(t值分别为2.62、4.03、3.43,P＜0.01).结论肝纤维化发生发展过程中,HIF-1α促进了TGF-β1、MMP-9等基因的转录表达.
목적 관찰결양유도인자-1α(HIF-1α)、기질금속단백매-9(MMP-9)화전화생장인자(TGF)-β1재대서간섬유화조직중적표체이급TGF-β1역묘대저사인자표체적영향.방법 이갑기아초알건립대서간섬유화모형,병여이TGF-β1역묘간예.재실험6주말용면역조직화학방법 검측HIF-1α、MMP-9화TGF-β1재대서간섬유화조직중적표체정황.조간비교채용단인소방차분석.방차제성자량량비교채용LSD법,방차불제자진행Dunnet's T3검험.결과여대조조비교,간섬유화모형조급TGF-β1역묘간예조HIF-1α、MMP-9、TGF-β1표체현저증가(P＜0.01),차여간섬유화정도정정상관관계(r치분별위0.911、0.814、0.836,P＜0.01);여간섬유화모형조비교,TGF-β1역묘간예조HIF-1α、MMP-9화TGF-β1표체현저강저(t치분별위2.62、4.03、3.43,P＜0.01).결론간섬유화발생발전과정중,HIF-1α촉진료TGF-β1、MMP-9등기인적전록표체.
Objective To observe the expression and significance of hypoxia inducible factor (HIF)-1α,matrix metalloproteinase(MMP)-9 and transforming growth factor(TGF)-β1 in liver tissues of rats with hepatic fibrosis and the effects of TGF-β1 vaccine on the expressions of HIF-1α,MMP-9 and TGF-β1.Methods The hepatic fibrosis rat model was set up by dimethylnitrosamine (DMN).The rat model was injected with TGF-β1 vaccine.The expressions of HIF-1α,MMP-9 and TGF-β1 were detected by immunohistochemistry in rat fibrosis tissues after 6 weeks.Results The expressions of HIF-1α,MMP-9 and TGF-β1 in fibrosis model group and TGF-β1 vaccine group were all significantly higher than control group(P＜0.01),which was positively correlated with the fibrosis degree(r=0.911,0.814 and 0.836,respectively,P＜0.01).However,the expressions of HIF-1α,MMP-9 and TGF-β1 in TGF-β1 vaccine group were all significantly decreased than fibrosis model group (t=2.62,4.03,3.43;P＜0.01).Conclusion During the development of liver fibrosis,HIF-1α promotes the transcriptions and expressions of MMP-9 and TGF-β1.