CAJ | 학술논문

目的探讨肌源性干细胞(muscle derived stem cells,MDSC)受转化生长因子β1(transforming growth factor-1,TCF-β1)刺激产生结缔组织生长因子(connective tissue growth factor,CTGF)的过程中,Erk信号转导通路和p38信号转导通路是否发挥作用.方法采用细胞差速贴壁法,从新生大鼠骨骼肌中分离提取肌源性干细胞,进行细胞表型鉴定后传代培养.实验设空白组、对照组和实验组共8组:空白组,添加液为基础培养基;对照组,基础培养基中加TGF-β1;PD98059实验组,分别用20、40、80μM的PD98059预处理;SB203580实验组,分别用0.2、0.5、1.0μM的SB203580预处理.用Real Time-PCR和Western Blot分别检测细胞中CTGF mRNA和蛋白质的水平.方果空白组、对照组和PD98059实验组间CTGF mRNA和蛋白质的相对表达量差异均有统计学意义(P<0.05),对照组和SB203580实验组间差异均无统计学意义(P>0.05).方论 TGF-β1诱导NDSC产生CTCF的过程中,存在着Erk信号转导途径,不存在p38信号转导途径.
목적 탐토기원성간세포(muscle derived stem cells,MDSC)수전화생장인자β1(transforming growth factor-1,TCF-β1)자격산생결체조직생장인자(connective tissue growth factor,CTGF)적과정중,Erk신호전도통로화p38신호전도통로시부발휘작용.방법 채용세포차속첩벽법,종신생대서골격기중분리제취기원성간세포,진행세포표형감정후전대배양.실험설공백조、대조조화실험조공8조:공백조,첨가액위기출배양기;대조조,기출배양기중가TGF-β1;PD98059실험조,분별용20、40、80μM적PD98059예처리;SB203580실험조,분별용0.2、0.5、1.0μM적SB203580예처리.용Real Time-PCR화Western Blot분별검측세포중CTGF mRNA화단백질적수평.방과 공백조、대조조화PD98059실험조간CTGF mRNA화단백질적상대표체량차이균유통계학의의(P<0.05),대조조화SB203580실험조간차이균무통계학의의(P>0.05).방론 TGF-β1유도NDSC산생CTCF적과정중,존재착Erk신호전도도경,불존재p38신호전도도경.
Objective To investigate the role of Erk and p38 pathway in the process of CTGF expression in TGF-β1-induced muscle-derived stem cells(MDSCs).Methods MDSCs were isolated from the skeletal muscle of neonatal rats.After phenotyping,these MDSCs were cultured under 8 conditions according to group assignment:normal control group(MDSCs+basic culture),negative control group(MDSCs+basic culture+TGF-β1),experimental group 1-3(MDSCs pretreated with 20,40 and 80 μM of PD98059 respectively+basic culture+TCF-β1),experimental group 4-6(NDSCs pretreated with 0.2,0.5 and 1.0 μM of SB203580+basic culture+TGF-β1).After 48 hours,the MDSCs CTGF mRNA expression and protein level were quantified with Real Time-PCR and Western Blot.Results There were statistically significant differences of CTCF expression levels among normal control group,negative control group and experimental group 1-3(P<0.05).There was no significant difference in CTGF expression between the negative control group and experimental group 4-6(P>0.05).Conclusion Erk plays a role in TGF-β1-CTCF signal transduction of rat NDSCs,whereas p38 does not.