CAJ | 학술논문

目的比较A、B和C3种类型CpG-ODN在动物模型中的疫苗佐剂活性,为设计高活性的新型人用疫苗佐剂提供理论基础.方法用不同CpG-ODN刺激体外培养的小鼠脾细胞,检测上清中的IFN-γ和IgM,从而确定3种类型的CpG-ODN.以基因工程乙肝表面抗原(HBsAg)为模型抗原、不同类型CpG-ODN为佐剂免疫BALB/c小鼠,用间接ELISA法检测被免动物血清中抗原特异性ISG、IgG1和IgG2a抗体.结果小鼠免疫结果表明,与Al(OH),对照组相比,3种类型CpG-ODN均具有良好的疫苗佐剂活性,但B和C型CpG-ODN诱生的抗原特异性IgG和IgG2a抗体水平远高于A型CpG-ODN.虽然A型CpG-ODN也能够显著增强总抗体水平,但并不改变IgG1和IgG2a抗体亚型的比值.这与B和C型CpG-ODN能够极大地促进TH1类免疫应答并降低IgG1/IgG2a比值明显不同,提示不同类型CpG-ODN可能通过不同机制起到疫苗佐剂作用.结论不同类型CpG-ODN具有不同疫苗佐剂活性,B和c型CpG.ODN在小鼠体内的体液免疫佐剂效果优于A型CpG.ODN.
목적 비교A、B화C3충류형CpG-ODN재동물모형중적역묘좌제활성,위설계고활성적신형인용역묘좌제제공이론기출.방법 용불동CpG-ODN자격체외배양적소서비세포,검측상청중적IFN-γ화IgM,종이학정3충류형적CpG-ODN.이기인공정을간표면항원(HBsAg)위모형항원、불동류형CpG-ODN위좌제면역BALB/c소서,용간접ELISA법검측피면동물혈청중항원특이성ISG、IgG1화IgG2a항체.결과 소서면역결과표명,여Al(OH),대조조상비,3충류형CpG-ODN균구유량호적역묘좌제활성,단B화C형CpG-ODN유생적항원특이성IgG화IgG2a항체수평원고우A형CpG-ODN.수연A형CpG-ODN야능구현저증강총항체수평,단병불개변IgG1화IgG2a항체아형적비치.저여B화C형CpG-ODN능구겁대지촉진TH1류면역응답병강저IgG1/IgG2a비치명현불동,제시불동류형CpG-ODN가능통과불동궤제기도역묘좌제작용.결론 불동류형CpG-ODN구유불동역묘좌제활성,B화c형CpG.ODN재소서체내적체액면역좌제효과우우A형CpG.ODN.
Objective To compare the adjuvanticity of type-A,B and C CpG-ODN in mice.Methods Three types of CpG-ODN were identified through in vitro stimulation of murine splenocytes with various CpG-ODN.BALB/c mice were immunized with HBsAg,together with different types of CpG-ODN,and antigen-specific IgG.IgG1 and IgG2a titers were measured 4 weeks later by indirect ELISA.Resuits Compared with the control group.all 3 types of CpG-ODN enhanced humoral immune response to HBsAg.However,type-B and C CpG-ODN induced much higher levels of antigen-specific IgG and IgG2a than type A CpG-ODN.Type-C CpG-ODN induced a similar TH 1-biased immune response as type-B CpG-ODN,revealed by decreased IsG1 to IgG2a ratio.In contrast,although type-A CpG-ODN increased IgG titers,it did not switch the balance between TH1 and TH2 immune responses.Conclusion All 3 types of CpG-ODN can enhance the humoral immune response to vaccines,but their aaiuvanticity could be mediated through different mechanisms.