CAJ | 학술논문

目的构建经不同转移途径的胆囊癌高转移亚群细胞体系,观察不同转移途径高转移细胞的生物学特征.方法将人胆囊癌细胞GBC-SD裸小鼠脾脏、足垫注射建立脾-脾静脉-肝转移模型、足垫-腹股沟淋巴结转移模型,转移灶中筛选血行途径和淋巴途径转移的亚群细胞.在形态学、遗传背景、细胞增殖、迁移、侵袭、黏附方面,比较淋巴转移、血行转移亚群细胞与亲代细胞的差异.结果筛选出血行转移亚群细胞GBC-SD/M3和淋巴转移亚群细胞GBC-SD/HL.与亲代细胞GBC-SD相比,血行转移亚群GBC-SD/M3具有上皮-间皮化形态改变(EMT),淋巴转移哑群GBC-SD/HL则无EMT样改变.血行转移亚群体外迁移能力最强,淋巴转移亚群在黏附力更具有优势.结论所构建的胆囊癌经不同转移途径的高转移细胞亚群包括淋巴转移哑群、血行转移亚群,连同亲代胆囊癌细胞GBC-SD,有同样的遗传背景,是胆囊癌的转移机制研究的理想细胞体系.EMT在胆囊癌血行转移中具有重要意义,而胆囊癌淋巴途径转移更依赖瘤细胞更强的黏附能力.
목적 구건경불동전이도경적담낭암고전이아군세포체계,관찰불동전이도경고전이세포적생물학특정.방법 장인담낭암세포GBC-SD라소서비장、족점주사건립비-비정맥-간전이모형、족점-복고구림파결전이모형,전이조중사선혈행도경화림파도경전이적아군세포.재형태학、유전배경、세포증식、천이、침습、점부방면,비교림파전이、혈행전이아군세포여친대세포적차이.결과 사선출혈행전이아군세포GBC-SD/M3화림파전이아군세포GBC-SD/HL.여친대세포GBC-SD상비,혈행전이아군GBC-SD/M3구유상피-간피화형태개변(EMT),림파전이아군GBC-SD/HL칙무EMT양개변.혈행전이아군체외천이능력최강,림파전이아군재점부력경구유우세.결론 소구건적담낭암경불동전이도경적고전이세포아군포괄림파전이아군、혈행전이아군,련동친대담낭암세포GBC-SD,유동양적유전배경,시담낭암적전이궤제연구적이상세포체계.EMT재담낭암혈행전이중구유중요의의,이담낭암림파도경전이경의뢰류세포경강적점부능력.
Objective To establish the serial cell lines, derived from the same parental gallbladder cancer cell line GBC-SD, with highly metastatic potential via different routes and characterize their biological behaviors to understand the different metastasis mechanisms via lymph and blood. Methods The spleenliver metastasis model and footpad-inguinal lymph node metastasis model were established. GBC-SD was injected into spleen or footpad of nude mice. Then the highly metastasized subpopulations via lymph and blood were isolated. Their differences in morphology, genetic background, proliferation, migration, invasion and adhesion were revealed by comparing the lymphatic-disseminating and hematogenous-disseminating subpopulations with parental cells. Results The lymphatic-disseminating and hematogenous-disseminating subpopulations were successfully isolated and designated as GBC-SD/HL and GBC-SD/M3 respectively.They demonstrated the identical genetic background with GBC-SD. In comparison with parental cells, the hematogenous-disseminating subpopulation was morphologically characterized with epithelial-mesenchymal transition (EMT) while it was not shown in the lymphatic-disseminating subpopulation. Furthermore, the hematogenous-disseminating subpopulation showed the strongest migrating capacity but the lymphaticdisseminating subpopulation demonstrated a stronger invasive and adhesive ability. Conclusion The whole parental cell GBC-SD, hematogenous-metastasized subpopulation GBC-SD/M3 and lymphatic-disseminating subpopulation GBC-SD/HL is an ideal tool for metastatic mechanism study of gallbladder cancer. EMT plays an important role in hematogenous metastasis while lymphatic metastasis relies more on enhanced invasiveness and adhesion. It may be a target for interfering the lymphatic metastasis of gallbladder cancer.