CAJ | 학술논문

学术背景:基因治疗是近年来全球研究的热点,其最大障碍是缺乏有效的基因载体.非病毒性载体比病毒性载体具有更高的安全性,因而越来越受到人们关注.壳聚糖具有优良的生物相容性、生物可降解性、低毒以及高正电荷.是一种良好的非病毒性基因载体.带正电荷的壳聚糖能与带负电荷的DNA形成聚电解质复合物,有效复合保护质粒DNA免遭DNase酶解,使基因得以传递和表达.目的:概述壳聚糖作基因载体及其改性研究所取得的有意义的最新研究进展.检索策略:应用计算机检索Science Direct,Springer,Wiley数据库和中国期刊全文数据库1991-01/2007-12的有关文献,检索关键词为"壳聚糖、基因载体、DNA、进展".共检索到425篇相关文献.其中英文文献217篇,中文文献208篇.对文献进行筛选,选取关键文章,对同一领域的文献选择近期发表或权威杂志的文献,排除重复研究的文章.最后70篇被选用.文献评价:所选用的70篇文献中,60篇为英文文献,10篇为中文文献;其中10篇为综述,其余均为实验研究论文.资料综合:壳聚糖有优良的生物相容性、生物可降解性,无免疫原性,是当今最具潜力的非病毒性基因载体之一.通过优化壳聚糖的分子质量、脱乙酰度、N/P比、血清浓度和介质的pH值等可对壳聚糖的转染效率和细胞吸收进行调控.化学改性壳聚糖,尤其是偶联配体可传递基因进入靶细胞.结论:改性后的壳聚糖可作为潜在的优良基因载体.
학술배경:기인치료시근년래전구연구적열점,기최대장애시결핍유효적기인재체.비병독성재체비병독성재체구유경고적안전성,인이월래월수도인문관주.각취당구유우량적생물상용성、생물가강해성、저독이급고정전하.시일충량호적비병독성기인재체.대정전하적각취당능여대부전하적DNA형성취전해질복합물,유효복합보호질립DNA면조DNase매해,사기인득이전체화표체.목적:개술각취당작기인재체급기개성연구소취득적유의의적최신연구진전.검색책략:응용계산궤검색Science Direct,Springer,Wiley수거고화중국기간전문수거고1991-01/2007-12적유관문헌,검색관건사위"각취당、기인재체、DNA、진전".공검색도425편상관문헌.기중영문문헌217편,중문문헌208편.대문헌진행사선,선취관건문장,대동일영역적문헌선택근기발표혹권위잡지적문헌,배제중복연구적문장.최후70편피선용.문헌평개:소선용적70편문헌중,60편위영문문헌,10편위중문문헌;기중10편위종술,기여균위실험연구논문.자료종합:각취당유우량적생물상용성、생물가강해성,무면역원성,시당금최구잠력적비병독성기인재체지일.통과우화각취당적분자질량、탈을선도、N/P비、혈청농도화개질적pH치등가대각취당적전염효솔화세포흡수진행조공.화학개성각취당,우기시우련배체가전체기인진입파세포.결론:개성후적각취당가작위잠재적우량기인재체.
Gene vectors are of great importance in gene therapy.Research on non-viral gene vectors has become the most urgent issue as viral gene vectors have shown a series of safety problems during clinical application.Chitosan is one of the best potential non-viral gene vectors because of its excellent physico-chemical and biological properties.In recent years,a lot of works have been done on chitosan and its modification for gene delivery,and valuable progresses have been achieved.In this paper,recent progresses in chitosan and its modified derivates for gene delivery are reviewed in temls of size,stability,specific targeting ability and gene transfection efficiency of the chitosan/gene complex.