CAJ | 학술논문

在生理酸度条件下(pH7.4),采用紫外-可见光谱法、荧光光谱法并结合溴化乙锭(EB)荧光探针、黏度测定、盐效应、磷酸盐效应和KI荧光猝火实验,研究倍硫磷与小牛胸腺DNA的相互作用.结果发现,DNA对倍硫磷内源性荧光产生强烈的猝灭作用,求得室温下倍硫磷-DNA间的结合常数和结合位点数分别为1.14×104L/mol和0.958.加入倍硫磷后DNA-EB复合物的荧光无显著猝灭,表明倍硫磷小能置换出DNA-EB复合物中的EB;倍硫磷的存在对DNA的相对黏度无明显影响;增加离子强度,倍硫磷自身及倍硫磷.DNA体系的荧光无明显变化;DNA的加入增大了Ⅰ-对倍硫磷荧光的猝灭程度.上述实验现象表明,倍硫磷通过沟区结合方式与DNA发生作用.
재생리산도조건하(pH7.4),채용자외-가견광보법、형광광보법병결합추화을정(EB)형광탐침、점도측정、염효응、린산염효응화KI형광졸화실험,연구배류린여소우흉선DNA적상호작용.결과발현,DNA대배류린내원성형광산생강렬적졸멸작용,구득실온하배류린-DNA간적결합상수화결합위점수분별위1.14×104L/mol화0.958.가입배류린후DNA-EB복합물적형광무현저졸멸,표명배류린소능치환출DNA-EB복합물중적EB;배류린적존재대DNA적상대점도무명현영향;증가리자강도,배류린자신급배류린.DNA체계적형광무명현변화;DNA적가입증대료Ⅰ-대배류린형광적졸멸정도.상술실험현상표명,배류린통과구구결합방식여DNA발생작용.
The interaction between fenthion and calf thymus DNA in physiological buffer (pH 7.4) was investigated by UV- vis spectroscopy, fluorescence spectroscopy, viscosity measurement, salt effect, phosphate effect and KI fluorescence quench- ing effect. The fluorescence intensity of fenthion was significantly reduced with the addition of DNA. The binding constant and number of binding sites of fenthion with DNA were calculated as being 1.14 ×104 L/mol and 0.958, respectively. There were no obvious fluorescence quenching effect found on the emission peak of DNA-ethidium bromide (EB) system when fenthion was added, suggesting that there is no competition for DNA binding between fenthion and EB. Neither the relative viscosity of DNA nor the fluorescence intensity of fenthion and fenthion-DNA showed significant changes with the addition of fenthion and sodium chloride. The quenching effect of Ⅰ- on the fluorescence intensity of fenthion was increased in the presence of DNA. Based on the above results, it can be inferred that fenthion and DNA interact in a groove-binding mode.