CAJ | 학술논문

采用超滤法对梅花鹿胎盘中活性寡肽进行分离,考察料液质量分数、压力、温度、流速、浓缩倍数及操作时间因素对分离效果的影响,确定最佳分离工艺条件为料液质量分数1.5%、压力0.06MPa、温度30℃、流速2.0ml/s、浓缩倍数2.5倍、操作时间3min,分离后的样品中蛋白含量为56.22%.膜污染后用0.2%NaOH溶液浸泡2h,循环1h后,用盐酸浸泡1h,循环0.5h,再用水洗至中性,膜通量可得到较好的恢复,流量恢复率为0.834.
채용초려법대매화록태반중활성과태진행분리,고찰료액질량분수、압력、온도、류속、농축배수급조작시간인소대분리효과적영향,학정최가분리공예조건위료액질량분수1.5%、압력0.06MPa、온도30℃、류속2.0ml/s、농축배수2.5배、조작시간3min,분리후적양품중단백함량위56.22%.막오염후용0.2%NaOH용액침포2h,순배1h후,용염산침포1h,순배0.5h,재용수세지중성,막통량가득도교호적회복,류량회복솔위0.834.
Active oligopeptides are the most effective ingredients in deer placenta. The ofigopeptides was separated through ultrafiltration from deer placenta. The major factors such as material-liquid ratio, pressure, temperature, flow rate, condensation folds and operation time were investigated to evaluate the effect on separation efficiency. The optimal separation condition was 1.5% of material-liquid ratio, 0.06 MPa of pressure, 30 ℃ of temperature, 2.0 ml/s of flow rate, 2.5 folds for condensation degree and 3 min of operation time. Protein content in separated samples was 56.22% under this optimal condition. The contaminated membrane was cleaned using 0.2% NaOH for 2 h of soaking and 1 h of circulation first, then using HCI for 1 h of soaking and 0.5 h of circulation, and finally using water washing until neutrality. An excellent recovery rate for membrane flux was achieved and WFR value was 0.834.