CAJ | 학술논문

背景:周围神经移植后,相应的背根神经节感觉神经元胞体内生长相关蛋白43(GAP-43)表达的时程与规律尚未明确.目的:观察大鼠坐骨神经移植术后GAP-43在相应背根神经节中的表达变化.方法:取健康成年雄性Wistar大鼠,随机分为2组,对照组行假手术组:实验组行坐骨神经移植,分别于术后3 d,1,2,4,6,8周6个时间点处死后取其手术侧L4~L5背根神经节,应用Fn-PCR和Westem Blot技术检测GAP-43 mRNA及其蛋白表达.结果与结论:对照组大鼠背根神经节中GAP-43 mRNA表达量较低,且随时间无明显改变;实验组术后1周时GAP-43 mRNA在相应背根神经节中即有表达增强,2周时达到高峰,6~8周表达逐渐减弱.两组大鼠背根神经节中GAP-43蛋白与GAP-43mRNA表达的变化规律相同.结果表明神经损伤后相应神经元的再生能力存在损伤反应性变化.
배경:주위신경이식후,상응적배근신경절감각신경원포체내생장상관단백43(GAP-43)표체적시정여규률상미명학.목적:관찰대서좌골신경이식술후GAP-43재상응배근신경절중적표체변화.방법:취건강성년웅성Wistar대서,수궤분위2조,대조조행가수술조:실험조행좌골신경이식,분별우술후3 d,1,2,4,6,8주6개시간점처사후취기수술측L4~L5배근신경절,응용Fn-PCR화Westem Blot기술검측GAP-43 mRNA급기단백표체.결과여결론:대조조대서배근신경절중GAP-43 mRNA표체량교저,차수시간무명현개변;실험조술후1주시GAP-43 mRNA재상응배근신경절중즉유표체증강,2주시체도고봉,6~8주표체축점감약.량조대서배근신경절중GAP-43단백여GAP-43mRNA표체적변화규률상동.결과표명신경손상후상응신경원적재생능력존재손상반응성변화.
BACKGROUND: Following peripheral nerve transplantation, the time phases and law of growth associated protein 43 (GAP-43) expression in sensory neuron soma of the corresponding dorsal root ganglia remain unclear.OBJECTIVE: To investigate the expression of GAP-43 at the corresponding dorsal root ganglia following sciatic nerve transplantation in rats.METHODS: Adult male Wistar rats were randomly divided into 2 groups: control group (sham-operated group) and experimental group (nerve transplantation group). The rats were killed at 3 days, 1, 2, 4, 6, and 8 weeks after operation and the dorsal root ganglia U-s of every rat was taken for observation. The expression of GAP-43 mRNA and protein in the corresponding segments of spinal cord were detected by RT-PCR and Western-blot techniques.RESULTS AND CONCLUSION: GAP-43 mRNA showed a low level of expression in the control group, which had no obvious changes in different phases. In the experimental group, GAP-43 mRNA expression was observed remarkably in the dorsal root ganglia at the first week, reached a peak at the second week and gradually descended from the sixth week. The GAP-43 protein expression showed the same laws as that of GAP-43 mRNA. The results demonstrated that there exists damage reactivity change in the regeneration ability of neurons.