CAJ | 학술논문

目的研究血管生成素2(Ang-2)基因在急性淋巴细胞白血病(ALL)中的表达及其临床意义.方法建市检测Ang-2基因的SYBR Green Ⅰ荧光实时定量聚合酶链反应(RQ-PCR)方法 ,对59例初发ALL患者骨髓细胞进行Ang-2表达水平的检测.同时选择20例非恶性血液病患者的骨髓细胞作为对照.结果 ALL及对照组骨髓细胞中均有Ang-2基因的表达,但在ALL患者的表达显著高于对照组(中位数分别为348.11、63.25,P<0.05).Ang-2表达水平与患者初诊时年龄、血红蛋白、白细胞、血小板、乳酸脱氢酶水平和外周血白血病细胞比例及骨髓白血病细胞比例等指标无明显相关(r=-0.066,r=-0.063,r=0.233,r=-0.145,r=0.105,r=-0.021,r=-0.190).Ang-2高表达组与低表达组的1年复发率、生存率比较差异无统计学意义(P>0.05).结论 Ang-2基因表达水平在ALL组高于非恶性血液病组,可能与ALL患者临床表现、治疗反应及预后无关.
목적 연구혈관생성소2(Ang-2)기인재급성림파세포백혈병(ALL)중적표체급기림상의의.방법 건시검측Ang-2기인적SYBR Green Ⅰ형광실시정량취합매련반응(RQ-PCR)방법 ,대59례초발ALL환자골수세포진행Ang-2표체수평적검측.동시선택20례비악성혈액병환자적골수세포작위대조.결과 ALL급대조조골수세포중균유Ang-2기인적표체,단재ALL환자적표체현저고우대조조(중위수분별위348.11、63.25,P<0.05).Ang-2표체수평여환자초진시년령、혈홍단백、백세포、혈소판、유산탈경매수평화외주혈백혈병세포비례급골수백혈병세포비례등지표무명현상관(r=-0.066,r=-0.063,r=0.233,r=-0.145,r=0.105,r=-0.021,r=-0.190).Ang-2고표체조여저표체조적1년복발솔、생존솔비교차이무통계학의의(P>0.05).결론 Ang-2기인표체수평재ALL조고우비악성혈액병조,가능여ALL환자림상표현、치료반응급예후무관.
Objective To study the expression levels and its clinical significance of angiopoietin 2 (Ang-2) gene in acute lymphatic leukemia(ALL). Methods The qualitative detection method was established with SYBR Green Ⅰ by real-time fluorescent quantitative PCR, and Ang-2 mRNA was measured in 51 cases of pre-therapeutic ALL. In addition, the expression of Ang-2 gene was also analyzed in 20 cases of non-malignant hematological diseases. Results The expression of Ang-2 gene was found in all the patients, and Ang-2 expression level in ALL patients was significantly higher than that of the controls (P<0.05). No significant relationship was found between Ang-2 expression level and clinical characteristics (age, haematogloblin, WBC count, platelet count, LDH, blast cells in peripheral blood, blast cells in bone marrow). The expression level of Ang-2 have no effect on one year replase rate and survival rate in ALL. Conclusion Ang-2 expression level in ALL patients was higher than that in non-malignant hematological diseases, and there may be no relationship with clinical manifestation, treatment and prognosis.