中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2012年
5期
315-317
,共3页
李玲杰%刘原君%姚卫锋%侯淑萍%尤聪%田敬群%冯斌%刘全忠
李玲傑%劉原君%姚衛鋒%侯淑萍%尤聰%田敬群%馮斌%劉全忠
리령걸%류원군%요위봉%후숙평%우총%전경군%풍빈%류전충
衣原体,沙眼%噬菌体%VP1蛋白
衣原體,沙眼%噬菌體%VP1蛋白
의원체,사안%서균체%VP1단백
Chlamydia trachomatis%Phages%Vp1 protein
目的 检测临床标本中沙眼衣原体噬菌体Vp1基因及血清Vp1抗体.方法 收集天津性传播疾病研究所就诊患者的分泌物拭子和血清；用PCR法筛查分泌物标本中的Vp1基因；以Vp1蛋白作为抗原通过ELISA法及Western印迹法检测血清中针对Vp1抗体的存在；对PCR法结果阳性的分泌物进行细胞培养,然后免疫荧光法进行检测Vp1.结果 共筛查出36例拭子的PCR扩增产物在目的片段位置出现明显条带,及23例Vp1抗体阳性的血清.通过细胞培养及免疫荧光法,利用制备的单抗检测沙眼衣原体临床标本,尚未发现阳性标本.结论 从临床拭子及血清中成功筛查出沙眼衣原体噬菌体Vp1基因和Vp1抗体.
目的 檢測臨床標本中沙眼衣原體噬菌體Vp1基因及血清Vp1抗體.方法 收集天津性傳播疾病研究所就診患者的分泌物拭子和血清；用PCR法篩查分泌物標本中的Vp1基因；以Vp1蛋白作為抗原通過ELISA法及Western印跡法檢測血清中針對Vp1抗體的存在；對PCR法結果暘性的分泌物進行細胞培養,然後免疫熒光法進行檢測Vp1.結果 共篩查齣36例拭子的PCR擴增產物在目的片段位置齣現明顯條帶,及23例Vp1抗體暘性的血清.通過細胞培養及免疫熒光法,利用製備的單抗檢測沙眼衣原體臨床標本,尚未髮現暘性標本.結論 從臨床拭子及血清中成功篩查齣沙眼衣原體噬菌體Vp1基因和Vp1抗體.
목적 검측림상표본중사안의원체서균체Vp1기인급혈청Vp1항체.방법 수집천진성전파질병연구소취진환자적분비물식자화혈청；용PCR법사사분비물표본중적Vp1기인；이Vp1단백작위항원통과ELISA법급Western인적법검측혈청중침대Vp1항체적존재；대PCR법결과양성적분비물진행세포배양,연후면역형광법진행검측Vp1.결과 공사사출36례식자적PCR확증산물재목적편단위치출현명현조대,급23례Vp1항체양성적혈청.통과세포배양급면역형광법,이용제비적단항검측사안의원체림상표본,상미발현양성표본.결론 종림상식자급혈청중성공사사출사안의원체서균체Vp1기인화Vp1항체.
Objective To detect Chlamydia trachomatis phage Vp1 gene in clinical swab specimens and anti-Vp1 antibodies in serum specimens.Methods Cervical and urethral swab as well as serum specimens were collected from attendees to the sexually transmitted disease (STD) clinic in the Tianjin Institute of STD,during March 2008 to March 2011.PCR was conducted to detect chlamydial phage Vp1 gene in swab samples,enzyme linked immunosorbent assay (ELISA) and Western blot to detect anti-Vp1 antibody in sera.The swab specimens positive for Vp1 gene were subjected to cell culture followed by the detection of Vp1 protein with an immunofluorescence-based method.Results Totally,36 out of 1542 swab specimens turned out to be positive for Vp1 gene,and 23 out of 453 serum specimens for anti-Vp1 antibody.No positive results were obtained in the Vp1 gene-positive swab specimens by cell culture and immunofluorescence-based assay.Conclusion The Vp1 gene of Chlamydial trachomatis phage and anti-Vp1 antibody are successfully detected from clinical swab and serum specimens respectively.
