中华内科杂志
中華內科雜誌
중화내과잡지
CHINESE JOURNAL OF INTERNAL MEDICINE
2011年
2期
107-110
,共4页
钱龙%史恒星%李向培%张宏%厉小梅%汪国生
錢龍%史恆星%李嚮培%張宏%厲小梅%汪國生
전룡%사항성%리향배%장굉%려소매%왕국생
关节炎,类风湿%肽酰基精氨酸脱亚氨酶4%多态性,单核苷酸
關節炎,類風濕%肽酰基精氨痠脫亞氨酶4%多態性,單覈苷痠
관절염,류풍습%태선기정안산탈아안매4%다태성,단핵감산
Arthritis,rheumatoid%Peptidylarginine deiminase 4%Polymorphism,single nucleotide
目的 通过检测肽酰基精氨酸脱亚氨酶(PADI)4在类风湿关节炎(RA)患者血清中的水平,探讨其在RA发病机制中的意义.方法 采用ELISA检测100例RA患者、23例其他风湿病患者、24例健康体检者血清中PADI4及抗环瓜氨酸多肽(CCP)抗体水平.结果 (1)RA患者PADI4水平为(620.10±259.82)μg/L,较其他风湿病患者[(404.41±195.38)μg/L]、健康体检者[(522.19±142.40)μg/L]明显升高,差异有统计学意义(F=8.75,P＜0.001).(2)RA患者PADI4-94(rs 2240340)基因型T/T、C/C、T/C PADI4水平分别为(597.43±255.76)μg/L、(674.63±320.67)/μgL、(628.78±274.13)μg/L;PADI4-104(RS 1748033)基因型T/T、Cμg/C、T/C PADI4水平分别为(571.56±138.34)μg/L、(653.41±300.35)μg/L、(640.07±302.82)μg/L.各基因型间PADI4水平差异无统计学意义(P＞0.05).(3)相关性分析:RA组PADI4水平与RA疾病活动评分(DAS28)、ESR呈正相关(r=0.24,P=0.03;r=0.23,P=0.03),而与抗CCP抗体、抗PADI4抗体、C反应蛋白无相关性(r=0.02,P=0.83;r=-0.17,P=0.10;r=0.14,P=0.18).结论 PADI4可能是RA的一个致病性抗原,参与了RA发病,其水平高低反映病情活动程度.
目的 通過檢測肽酰基精氨痠脫亞氨酶(PADI)4在類風濕關節炎(RA)患者血清中的水平,探討其在RA髮病機製中的意義.方法 採用ELISA檢測100例RA患者、23例其他風濕病患者、24例健康體檢者血清中PADI4及抗環瓜氨痠多肽(CCP)抗體水平.結果 (1)RA患者PADI4水平為(620.10±259.82)μg/L,較其他風濕病患者[(404.41±195.38)μg/L]、健康體檢者[(522.19±142.40)μg/L]明顯升高,差異有統計學意義(F=8.75,P＜0.001).(2)RA患者PADI4-94(rs 2240340)基因型T/T、C/C、T/C PADI4水平分彆為(597.43±255.76)μg/L、(674.63±320.67)/μgL、(628.78±274.13)μg/L;PADI4-104(RS 1748033)基因型T/T、Cμg/C、T/C PADI4水平分彆為(571.56±138.34)μg/L、(653.41±300.35)μg/L、(640.07±302.82)μg/L.各基因型間PADI4水平差異無統計學意義(P＞0.05).(3)相關性分析:RA組PADI4水平與RA疾病活動評分(DAS28)、ESR呈正相關(r=0.24,P=0.03;r=0.23,P=0.03),而與抗CCP抗體、抗PADI4抗體、C反應蛋白無相關性(r=0.02,P=0.83;r=-0.17,P=0.10;r=0.14,P=0.18).結論 PADI4可能是RA的一箇緻病性抗原,參與瞭RA髮病,其水平高低反映病情活動程度.
목적 통과검측태선기정안산탈아안매(PADI)4재류풍습관절염(RA)환자혈청중적수평,탐토기재RA발병궤제중적의의.방법 채용ELISA검측100례RA환자、23례기타풍습병환자、24례건강체검자혈청중PADI4급항배과안산다태(CCP)항체수평.결과 (1)RA환자PADI4수평위(620.10±259.82)μg/L,교기타풍습병환자[(404.41±195.38)μg/L]、건강체검자[(522.19±142.40)μg/L]명현승고,차이유통계학의의(F=8.75,P＜0.001).(2)RA환자PADI4-94(rs 2240340)기인형T/T、C/C、T/C PADI4수평분별위(597.43±255.76)μg/L、(674.63±320.67)/μgL、(628.78±274.13)μg/L;PADI4-104(RS 1748033)기인형T/T、Cμg/C、T/C PADI4수평분별위(571.56±138.34)μg/L、(653.41±300.35)μg/L、(640.07±302.82)μg/L.각기인형간PADI4수평차이무통계학의의(P＞0.05).(3)상관성분석:RA조PADI4수평여RA질병활동평분(DAS28)、ESR정정상관(r=0.24,P=0.03;r=0.23,P=0.03),이여항CCP항체、항PADI4항체、C반응단백무상관성(r=0.02,P=0.83;r=-0.17,P=0.10;r=0.14,P=0.18).결론 PADI4가능시RA적일개치병성항원,삼여료RA발병,기수평고저반영병정활동정도.
Objective To detect the levels of serum peptidylarginine deiminase (PADI)4 and explore its significance in rheumatoid arthritis ( RA ). Methods The presence of PADI4, anti-PADI4antibodies and anti-cyclic citrullinated peptide (CCP) antibodies levels were examined by enzyme-linked immunosorbent assay (ELISA). Serum samples were obtained from 100 patients with RA, 23 patients with other rheumatic diseases, and 24 healthy controls. The associations between PADI4 and the disease activity score using 28 joint counts ( DAS28 ) score, anti-CCP antibodies, erythrocyte sedimentation rate ( ESR ),C-reactive protein ( CRP), PADI4-94 ( rs 2240340 ) and PADI-104 ( rs 1748033 ) gene polymorphisms and other indexes were analyzed in RA. Results The levels of PADI4 in RA patients were significantly higher than in other rheumatic diseases and healthy controls ( F = 8. 75, P ＜ 0. 001 ). There was no significantly difference in levels of PADI4 among ADI4-94 ( rs 2240340)/PADI-104 ( rs 1748033 ) genotypes. Conclusions PADI4 is associated with disease activity involved in the RA pathogenesis and may be a RA pathogenic antigen.
