CAJ | 학술논문

目的探讨微量离心柱法检测甲胎蛋白异质体在肝癌预警及良恶性肝病鉴别诊断中的临床价值.方法应用装有耦联小扁豆凝集素(LCA)的微量离心柱分离300例肝病患者的AFP-L3,采用化学发光法检测AFP及AFP-L3,计算AFP-L3占总AFP的比率(判断标准以AFP-L3≥10%者为阳性).结果 AFP-L3在肝细胞癌患者组中的阳性率是95%,在慢性肝病患者组的阳性率是64%,两组患者AFP-L3阳性率差异有统计学意义(x2=134.72,P＜0.01);AFP-L3阳性的慢性肝病患者与阴性患者肝癌发生率差异有统计学意义(x2=80.158,P＜0.01);AFP-L3的百分比与AFP浓度不相关(r=0.046,P＞0.05).结论微量离心柱法检测甲胎蛋白异质体(AFP-L3)在肝细胞癌诊断、预警及与良恶性肝病的鉴别诊断中具有重要价值.
목적 탐토미량리심주법검측갑태단백이질체재간암예경급량악성간병감별진단중적림상개치.방법 응용장유우련소편두응집소(LCA)적미량리심주분리300례간병환자적AFP-L3,채용화학발광법검측AFP급AFP-L3,계산AFP-L3점총AFP적비솔(판단표준이AFP-L3≥10%자위양성).결과 AFP-L3재간세포암환자조중적양성솔시95%,재만성간병환자조적양성솔시64%,량조환자AFP-L3양성솔차이유통계학의의(x2=134.72,P＜0.01);AFP-L3양성적만성간병환자여음성환자간암발생솔차이유통계학의의(x2=80.158,P＜0.01);AFP-L3적백분비여AFP농도불상관(r=0.046,P＞0.05).결론 미량리심주법검측갑태단백이질체(AFP-L3)재간세포암진단、예경급여량악성간병적감별진단중구유중요개치.
Objective To explore the clinical value of Lens culinaris agglutinin-reactive alphafetoprotein in the differentiation diagnosis between benign and malignant liver diseases, as well as the early warning of hepatocellular carcinoma. Methods Alpha-fetoprotein variants from 300 patients with liver diseases were isolated with micro-spin column equipped lens culinaris agglutinin (LCA). The AFP and AFP-L3 were detected by the electrochemical luminescence (ECL) method, and the proportions of AFP-L3 were calculated. Results The positive rates of AFP-L3 of HCC patients and chronic liver disease patients were 95% and 64% respectively, there were significant difference in two groups (x2 = 134.72, P ＜ 0. 01 ),the HCC incidence rates of AFP-L3 positive and negative chronic liver disease patients showed significant difference(x2 = 80. 158, P ＜ 0.01 ). there were no correlations between the proportion of AFP-L3 and AFP consistency(r = 0.046,P ＞0.05). Conclusions The detection of AFP-L3 by micro-spin column assay show great clinical value in the differentiation diagnosis of benign and malignant liver diseases, as well as the early warning of hepatocellular carcinoma.