CAJ | 학술논문

目的调查研究中国狂犬病高发地区狂犬病毒磷蛋白P基因遗传变异情况,分析其结构特点.方法测定广西、贵州、湖南的人、犬脑组织标本狂犬病毒P基因核苷酸序列,进行序列的同源性比较和种系发生分析.结果 P基因核苷酸和推导氨基酸序列同源性分别为82.1%~100%和87.5%~100%;三省区毒株明显区别于其他国家地区分离株;PP主要功能位点未发生影响P基因生物学功能的变异.结论三省区狂犬病毒同属于基因1型,具有共同的进化途径和基因结构特点;病毒分布具有独特的中国地域性特征;中国湖南省个别毒株可能和泰国毒株来源于共同的狂犬病毒.
목적 조사연구중국광견병고발지구광견병독린단백P기인유전변이정황,분석기결구특점.방법 측정엄서、귀주、호남적인、견뇌조직표본광견병독P기인핵감산서렬,진행서렬적동원성비교화충계발생분석.결과 P기인핵감산화추도안기산서렬동원성분별위82.1%~100%화87.5%~100%;삼성구독주명현구별우기타국가지구분리주;PP주요공능위점미발생영향P기인생물학공능적변이.결론 삼성구광견병독동속우기인1형,구유공동적진화도경화기인결구특점;병독분포구유독특적중국지역성특정;중국호남성개별독주가능화태국독주래원우공동적광견병독.
Objective Characterization of rabies virus phosphoprotein through analyzation of genetic variations about rabies virus phosphoproteins in high-incidence regions in China. Methods The nucleotide sequence of the P gene of Guangxi, Guizhou and Hunan provinces positive sample's were sequenced, and the P region's similarity and phylogenetic analyses were completed by using softer wares. Results The similarity of P region's nucleotide sequence is 82.1%-100%, while, the similarity of amino acid sequence is 87.5%-100%. A little variation in phosphoprotein cannot influence its biological functions. Conclusion All rabies viruses isolated from Guangxi, Guizhou and Hunan provinces belong to genotype 1 and share same phylogenies and same genome characteristic; Virus distribution presents unique Characterization; Some virus isolates from Hunan province and Thailand may come from the same virus.