CAJ | 학술논문

目的探讨纳米氧化铁(Fe3 O4)双歧杆菌脂磷壁酸(Bigidobacterium lipoteichoic acid,BLTA)对人胃癌荷瘤鼠体内细胞因子水平的影响,研究以纳米Fe3 O4为载体的BLTA(Fe3 O4-BLTA)对BGC-823胃癌移植瘤的抑制机制. 方法用人低分化BGC-823胃癌细胞株移植到40只雄性BALB/c裸鼠建立胃癌荷瘤鼠模型.将随机分组的荷瘤鼠,进行不同浓度的纳米Fe3 O4-BLTA复合物灌胃12 d后处死.取移植瘤观察其肿瘤抑制率,应用免疫组化技术,检测瘤体内CD31微血管密度值(microvessel density,MVD)及血管内皮生长因子(VEGF)的阳性率.提取腹腔巨噬细胞,用ELISA法检测细胞因子含量. 结果纳米Fe3O4-BLTA低剂量组(10μ/d)对肿瘤的抑制率达到49%;纳米Fe3 O4-BLTA低剂量组的CD31-MVD(57.000±6.790)与VEGF的表达(O.0224士0.0763)均比其他剂量组低(P<O.01),VEGF的表达水平与肿瘤生长的抑制率呈负相关;纳米Fe3 O4-BLTA高剂量组(100μg/d)和中剂量组(50μg/d)刺激巨噬细胞分泌细胞因子的差异有统计学意义(P<0.01).纳米Fe3 O4-BLTA低剂量组诱导TNF-α咀水平较其他剂量组相对较高(39.4040±I.5590,P<0.01). 结论纳米Fe3 O4-BLTA复合物可诱导一些肿瘤相关因子和免疫细胞因子,与阴性对照组相比,在12 d内对胃癌病灶鼠的肿瘤抑制效果明显(P<O.01).BLTA的纳米载体含量对机体吸收LTA有一定的影响.纳米Fe3O4-BLTA可利用其具有磁性的纳米级微粒,靶向定位于作用位点,作为靶向治疗胃癌新药物的研发对象.
목적 탐토납미양화철(Fe3 O4)쌍기간균지린벽산(Bigidobacterium lipoteichoic acid,BLTA)대인위암하류서체내세포인자수평적영향,연구이납미Fe3 O4위재체적BLTA(Fe3 O4-BLTA)대BGC-823위암이식류적억제궤제. 방법 용인저분화BGC-823위암세포주이식도40지웅성BALB/c라서건립위암하류서모형.장수궤분조적하류서,진행불동농도적납미Fe3 O4-BLTA복합물관위12 d후처사.취이식류관찰기종류억제솔,응용면역조화기술,검측류체내CD31미혈관밀도치(microvessel density,MVD)급혈관내피생장인자(VEGF)적양성솔.제취복강거서세포,용ELISA법검측세포인자함량. 결과 납미Fe3O4-BLTA저제량조(10μ/d)대종류적억제솔체도49%;납미Fe3 O4-BLTA저제량조적CD31-MVD(57.000±6.790)여VEGF적표체(O.0224사0.0763)균비기타제량조저(P<O.01),VEGF적표체수평여종류생장적억제솔정부상관;납미Fe3 O4-BLTA고제량조(100μg/d)화중제량조(50μg/d)자격거서세포분비세포인자적차이유통계학의의(P<0.01).납미Fe3 O4-BLTA저제량조유도TNF-α저수평교기타제량조상대교고(39.4040±I.5590,P<0.01). 결론 납미Fe3 O4-BLTA복합물가유도일사종류상관인자화면역세포인자,여음성대조조상비,재12 d내대위암병조서적종류억제효과명현(P<O.01).BLTA적납미재체함량대궤체흡수LTA유일정적영향.납미Fe3O4-BLTA가이용기구유자성적납미급미립,파향정위우작용위점,작위파향치료위암신약물적연발대상.
Objective To investigate the effect of lipoteichoic acid from Bifidobacterium loaded with magnetic particle nano-sized-Fe3O4(nano-sized-Fe3O4-BLTA)on several cytokines in vivo expression in beterologous graft model for human gastric cancer in nude mice.and to analyze the inhibition mechanism of nano-sized-Fe3 O4-BLTA on BGC-823 human gastric carcinoma transplanted tumor.Methods Forty male nude mice(BALB/e.nu/nu)were used for in vivo transplant tumor model.the experimental animals were randomly divided into five groups to administrated by several dosages of Hano-sized-Fe3 O4-BLTA,and were executed after 12 d.The tumors were collected and photographed.and the tumor tissues were used for differ-ent assays for measuring tumor inhibition.The peritoneal fluid was extracted to isolate the macrophages for cytokines assays.Using irmnunohistochemical staining for vascular endothelial growth factor(VEGF)and CD3 l in tumor to investigate the tumor inhibition rate.Double antibody sandwich ELISA was used to detect the level of cytokine change.Results The mice treated with low dosage(10 μg/d)of nano-sized-Fe3O4-BLTA.the growth inhibifion rate of tumor was 49%,and the levels of VEGF(0.0224±0.0763)and CD31 (57.000 4±6.790)were lower than other treated groups(P<0.01).The high dosage(100μg/d)and me-dium dosage(50μg/d)of nano-sized-Fe3 O4-BLTA groups were significant difference(P<0.01)on the content of cytokines excreted by macrophages.The level of TNF-α(39.4040 ±-1.5590)induced by the low dosage group was higher relatively(P<0.01).Conclusion Nano-sized-Fe3O4-BLTA exerts an inhibiting effect on the growth of human gastric cancer in nude mice.Using nano-sited-Fe3 O4-BLTA,LTA can get more permeability and improve the therapy effect.which will be a new drug on the stomach cancer targeted therapy.