CAJ | 학술논문

菊花/化学%菊花/药理学%乙酸盐类%植物提取物/药理学%心肌再灌注损伤/病理生理学%心律失常/中药疗法%心室颤动/中药疗法%动作电位菊花/化學%菊花/藥理學%乙痠鹽類%植物提取物/藥理學%心肌再灌註損傷/病理生理學%心律失常/中藥療法%心室顫動/中藥療法%動作電位
국화/화학%국화/약이학%을산염류%식물제취물/약이학%심기재관주손상/병리생이학%심률실상/중약요법%심실전동/중약요법%동작전위
Chrysanthemum Morifolium Ramat/chem%Chrysanthemum Morifolium Ramat/pharmacol%Acetates%Plant extracts/pharmacol%Myocardial reperfusion injury/physiopathol%Arrhythmia/ZHONG YAO LIAO FA%Ventricular fibrillation/ZHONG YAO LIAO FA%Action potentials

目的:研究杭白菊乙酸乙酯提取物(CME)对大鼠实验性心律失常、心肌易损性与动作电位的影响及其机制.方法:采用乌头碱诱发的整体大鼠心律失常模型研究CME对大鼠实验性心律失常的影响.采用Langendorff离体心脏灌流方法,结扎冠状动脉左前降支30 min后复灌复制局部缺血/复灌模型,测定心肌缺血复灌前后的心室电生理学参数:舒张期兴奋阈(DET)、有效不应期(ERP)、室颤阈(VFT).采用常规微电极技术记录大鼠右心室乳头肌动作电位,观测静息电位(RP)、动作电位幅度(APA)、有效不应期(ERP)、动作电位的时程(APD90)、动作电位0期最大除极速率(Vmax).结果:与对照组相比,CME明显降低室性心动过速发生次数,缩短其持续时间,延迟室性早搏、室性心动过速出现时间,心律失常评分显著降低.与对照组相比,CME明显延长离体大鼠心脏的ERP,并对缺血/复灌所致的ERP缩短和VFT降低有明显的减弱作用.与对照组相比,CME明显延长大鼠右室乳头肌动作电位APD50和APD90,降低动作电位Vmax,对动作电位的其他参数影响不显著.结论:CME具有降低大鼠心室易颤性、抗心律失常的作用,其机制可能涉及延长心肌动作电位时程及有效不应期,提高大鼠心脏电生理稳定性.
목적:연구항백국을산을지제취물(CME)대대서실험성심률실상、심기역손성여동작전위적영향급기궤제.방법:채용오두감유발적정체대서심률실상모형연구CME대대서실험성심률실상적영향.채용Langendorff리체심장관류방법,결찰관상동맥좌전강지30 min후복관복제국부결혈/복관모형,측정심기결혈복관전후적심실전생이학삼수:서장기흥강역(DET)、유효불응기(ERP)、실전역(VFT).채용상규미전겁기술기록대서우심실유두기동작전위,관측정식전위(RP)、동작전위폭도(APA)、유효불응기(ERP)、동작전위적시정(APD90)、동작전위0기최대제겁속솔(Vmax).결과:여대조조상비,CME명현강저실성심동과속발생차수,축단기지속시간,연지실성조박、실성심동과속출현시간,심률실상평분현저강저.여대조조상비,CME명현연장리체대서심장적ERP,병대결혈/복관소치적ERP축단화VFT강저유명현적감약작용.여대조조상비,CME명현연장대서우실유두기동작전위APD50화APD90,강저동작전위Vmax,대동작전위적기타삼수영향불현저.결론:CME구유강저대서심실역전성、항심률실상적작용,기궤제가능섭급연장심기동작전위시정급유효불응기,제고대서심장전생리은정성.
Objective: To investigate the effect of ethyl acetate extract from Chrysanthemum Morifolium Ramat (CME) on experimental arrhythmia induced by ischemia/reperfusion or aconitine in rats and to explore its underlying mechanisms. Methods: Arrhythmia model in intact rat was induced by aconitine (30 μg/kg body weight,i.v.).In isolated Langendorff perfused rat hearts,regional ischemia and reperfusion was induced by ligation and release of left anterior descending artery.The ventricular fibrillation threshold (VFT),effective refractory period (ERP),and diastolic excitation threshold (DET) in the isolated heart were measured.The action potentials of papillary muscle in rat right ventricle were recorded by conventional glass microelectrode technique. Results: Compared with control group CME significantly decreased the number and duration of ventricular tachycardia (VT);delayed the occurrence of ventricular premature beats (VPB) and VT induced by aconitine.Arrhythmia score of the CME group was lower than that in aconitine-treated group.CME markedly prolonged the ERP and increased the VFT in the isolated perfused rat hearts during ischemia and reperfusion.CME prolonged action potential duration at 50% and 90% repolarization of the right ventricular papillary muscles and decreased the maximal rate of rise of the action potential upstroke,but did not affect the resting potential,amplitude of action potential. Conclusion: CME can reduce myocardial vulnerability and exerts its antiarrhythmic effects induced by aconitine or ischemia/reperfusion,which may be related to its prolongation of action potential duration and effective refractory period that enhance the electrophysiological stability of myocardiaium.