国际放射医学核医学杂志
國際放射醫學覈醫學雜誌
국제방사의학핵의학잡지
INTERNATIONAL JOURNAL OF RADIATION MEDICINE AND NUCLEAR MEDICINE
2010年
3期
139-142
,共4页
李亚军%白人驹%高硕%李彦生%刘磊%贾薇%蔡莉%邢喜玲
李亞軍%白人駒%高碩%李彥生%劉磊%賈薇%蔡莉%邢喜玲
리아군%백인구%고석%리언생%류뢰%가미%채리%형희령
肺肿瘤%正电子发射断层显像%氟脱氧葡萄糖F18%11C-胆碱%模型,动物%兔%微血管密度
肺腫瘤%正電子髮射斷層顯像%氟脫氧葡萄糖F18%11C-膽堿%模型,動物%兔%微血管密度
폐종류%정전자발사단층현상%불탈양포도당F18%11C-담감%모형,동물%토%미혈관밀도
Lung neoplasms%Positron-emission tomopraphy%Fluorodeoxyglucose F18%11C-choline%Model,animal%Rabbits%Microvessel density
目的 探讨11C-胆碱和18F-FDG PET-CT反映兔VX2肺肿瘤血管生成的效能.方法 新西兰大白兔54只,右肺接种VX2肿瘤10～11 d后,行11C-胆碱和18F-FDG PET-CT,测量肿瘤11C-胆碱和18F-FDG最大标准化摄取值(SUVmax).同时,测量肿瘤标本大小,用免疫组化方法评价肿瘤微血管密度(MVD).然后对肿瘤胆碱SUVmax、18F-FDG SUVmax与肿瘤大小、MVD进行相关性分析.结果 33只实验兔成功完成所有检查,得到Vχ2肺肿瘤的11C-胆碱SUVmax平均值为4.02±3.07(1.4～12.2),对18F-FDG的SUVmax平均值为5.70±3.45(1.0～13.0),肿瘤大小平均值为(1.68±1.61)cm3(0.13～8.00 cm3).高倍镜(200倍,0.739 mm2)视野下,肿瘤MVD平均值为(35.8±13.6)个(13～64个).经统计学分析,11C-胆碱SUVmax与肿瘤大小及MVD之间均不具有相关性;18F-FDG SUVmax与MVD(r=0.525,P=0.002)之间存在正相关关系,与肿瘤大小(r=0.335,P=0.057)之间呈临界正相关关系.结论 18F-FDG PET-CT可反映兔VX2肺肿瘤血管生成,11C-胆碱PET-CT不能反映肿瘤血管生成.
目的 探討11C-膽堿和18F-FDG PET-CT反映兔VX2肺腫瘤血管生成的效能.方法 新西蘭大白兔54隻,右肺接種VX2腫瘤10～11 d後,行11C-膽堿和18F-FDG PET-CT,測量腫瘤11C-膽堿和18F-FDG最大標準化攝取值(SUVmax).同時,測量腫瘤標本大小,用免疫組化方法評價腫瘤微血管密度(MVD).然後對腫瘤膽堿SUVmax、18F-FDG SUVmax與腫瘤大小、MVD進行相關性分析.結果 33隻實驗兔成功完成所有檢查,得到Vχ2肺腫瘤的11C-膽堿SUVmax平均值為4.02±3.07(1.4～12.2),對18F-FDG的SUVmax平均值為5.70±3.45(1.0～13.0),腫瘤大小平均值為(1.68±1.61)cm3(0.13～8.00 cm3).高倍鏡(200倍,0.739 mm2)視野下,腫瘤MVD平均值為(35.8±13.6)箇(13～64箇).經統計學分析,11C-膽堿SUVmax與腫瘤大小及MVD之間均不具有相關性;18F-FDG SUVmax與MVD(r=0.525,P=0.002)之間存在正相關關繫,與腫瘤大小(r=0.335,P=0.057)之間呈臨界正相關關繫.結論 18F-FDG PET-CT可反映兔VX2肺腫瘤血管生成,11C-膽堿PET-CT不能反映腫瘤血管生成.
목적 탐토11C-담감화18F-FDG PET-CT반영토VX2폐종류혈관생성적효능.방법 신서란대백토54지,우폐접충VX2종류10～11 d후,행11C-담감화18F-FDG PET-CT,측량종류11C-담감화18F-FDG최대표준화섭취치(SUVmax).동시,측량종류표본대소,용면역조화방법평개종류미혈관밀도(MVD).연후대종류담감SUVmax、18F-FDG SUVmax여종류대소、MVD진행상관성분석.결과 33지실험토성공완성소유검사,득도Vχ2폐종류적11C-담감SUVmax평균치위4.02±3.07(1.4～12.2),대18F-FDG적SUVmax평균치위5.70±3.45(1.0～13.0),종류대소평균치위(1.68±1.61)cm3(0.13～8.00 cm3).고배경(200배,0.739 mm2)시야하,종류MVD평균치위(35.8±13.6)개(13～64개).경통계학분석,11C-담감SUVmax여종류대소급MVD지간균불구유상관성;18F-FDG SUVmax여MVD(r=0.525,P=0.002)지간존재정상관관계,여종류대소(r=0.335,P=0.057)지간정림계정상관관계.결론 18F-FDG PET-CT가반영토VX2폐종류혈관생성,11C-담감PET-CT불능반영종류혈관생성.
Objective To evaluate and compare the suitability of 11C-choline and 18F-FDG PET-CT for reflecting tumors angiogenesis. Methods Fifty-four New Zealand white rabbits which weighted 2.5～3.0 kg were used in the experiment. Under general anesthesia, a needle was transthoracically inserted into the right lung, 0.5 ml viable VX2 tumor cell suspension was slowly injected through the needle to establish the model.11C-choline and 18F-FDG PET-CT were performed after 10～11 d. The tumors SUVmax were calculated. The sections were stained with hematoxylin and eosin, and immunostained for CD34. Assessment of microvessel density(MVD) was performed by computer-assisted image analysis. The relationship 11C-choline SUVmax and 18F-FDG SUVmax with tumor size and MVD were statistically analyzed. Results Thirty-three rabbits successfully completed all imaging examinations, 11C-choline and 18F-FDG differently accumulated in all lung VX2 tumors. The mean of 11C-choline SUVmax was 4.02±3.07 (1.4～12.2), and the mean of 18-FDG SUVmax was 5.70±3.45 (1.0～13.0). The mean size of tumor was ( 1.68±1.61 )cm3 (0.13～8.00 cm3). Under high power microscope field of vision (200×, 0.739 mm2), the mean of MVD was 35.8±13.6 (13～64). 11C-choline SUVmaxdid not correlate with tumor size and MVD. 18F-FDG SUVmax significantly and positively related to MVD (r=0.525, P=0.002). There was a critical positive correlation between 18F-FDG SUVmax and tumor size (r=0.335,P=0.057). Conclusions In the rabbit VX2 lung tumor model, 18F-FDG SUVmax correlated with MVD, so 18F-FDG PET-CT could reflect tumor angiogenesis, 11C-choline SUVmax did not statistically correlate with MVD,and 11C-choline PET-CT could not reflect tumor angiogenesis.
