国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2010年
7期
409-413
,共5页
姜华%赖克方%沈璐%洪燕华%周银波%罗炜%陈如冲%钟南山
薑華%賴剋方%瀋璐%洪燕華%週銀波%囉煒%陳如遲%鐘南山
강화%뢰극방%침로%홍연화%주은파%라위%진여충%종남산
卡介菌多糖核酸%哮喘%气道反应性%嗜酸粒细胞
卡介菌多糖覈痠%哮喘%氣道反應性%嗜痠粒細胞
잡개균다당핵산%효천%기도반응성%기산립세포
Bacillus Calmette-Guérin polysaccharide nucleotide%Asthma%Airway hyperresponsiveness%Eosinophil
目的 探讨卡介菌多糖核酸对哮喘小鼠气道反应性及气道炎症的影响.方法 选择Balb/c小鼠,以卵白蛋白致敏激发建立小鼠哮喘模型,设立卡介菌多糖核酸组、正常组、哮喘组.卡介菌多糖核酸按剂量具体分为1 μg,10 μg,100 μg亚组,均在第一次抗原致敏前7 d腹腔注射给药.末次激发后48 h采用美国Buxco公司小鼠整体体积扫描记法测定气道反应性,以乙酰甲胆碱各浓度激发时增强的呼吸间歇(Enhanced Pause,Penh)表示.以PC100[气道反应性升高为生理盐水(NS)值2倍时的Mch激发浓度]及Penh/NS%max综合评价气道反应性.收集支气管肺泡灌洗液,涂片后苏木素-伊红染色计数嗜酸粒细胞比例,肺组织病理检测.结果 1μg肛组PC100(13.2±6.9)g/L、10/μg组(11.8±5.58)g/L与哮喘组(5.97±1.73)g/L相比,P<0.01表示差异有统计学意义;1 μg、10 μg组Penh/NS%max分别为(623.22±252.39)%,(519.71±200.41)%,显著低于哮喘组(1 306.83±540.46)%,P<0.01.10 μg组BALF嗜酸粒细胞百分比为(42.75±7.44)%显著低于哮喘组(57.25±13.2)%,P<0.01,1 μg组、100 μg组BALF嗜酸粒细胞百分比与哮喘组相比,差异无统计学意义.结论 卡介菌多糖核酸可以显著抑制哮喘小鼠的气道高反应性及气道炎症.
目的 探討卡介菌多糖覈痠對哮喘小鼠氣道反應性及氣道炎癥的影響.方法 選擇Balb/c小鼠,以卵白蛋白緻敏激髮建立小鼠哮喘模型,設立卡介菌多糖覈痠組、正常組、哮喘組.卡介菌多糖覈痠按劑量具體分為1 μg,10 μg,100 μg亞組,均在第一次抗原緻敏前7 d腹腔註射給藥.末次激髮後48 h採用美國Buxco公司小鼠整體體積掃描記法測定氣道反應性,以乙酰甲膽堿各濃度激髮時增彊的呼吸間歇(Enhanced Pause,Penh)錶示.以PC100[氣道反應性升高為生理鹽水(NS)值2倍時的Mch激髮濃度]及Penh/NS%max綜閤評價氣道反應性.收集支氣管肺泡灌洗液,塗片後囌木素-伊紅染色計數嗜痠粒細胞比例,肺組織病理檢測.結果 1μg肛組PC100(13.2±6.9)g/L、10/μg組(11.8±5.58)g/L與哮喘組(5.97±1.73)g/L相比,P<0.01錶示差異有統計學意義;1 μg、10 μg組Penh/NS%max分彆為(623.22±252.39)%,(519.71±200.41)%,顯著低于哮喘組(1 306.83±540.46)%,P<0.01.10 μg組BALF嗜痠粒細胞百分比為(42.75±7.44)%顯著低于哮喘組(57.25±13.2)%,P<0.01,1 μg組、100 μg組BALF嗜痠粒細胞百分比與哮喘組相比,差異無統計學意義.結論 卡介菌多糖覈痠可以顯著抑製哮喘小鼠的氣道高反應性及氣道炎癥.
목적 탐토잡개균다당핵산대효천소서기도반응성급기도염증적영향.방법 선택Balb/c소서,이란백단백치민격발건립소서효천모형,설립잡개균다당핵산조、정상조、효천조.잡개균다당핵산안제량구체분위1 μg,10 μg,100 μg아조,균재제일차항원치민전7 d복강주사급약.말차격발후48 h채용미국Buxco공사소서정체체적소묘기법측정기도반응성,이을선갑담감각농도격발시증강적호흡간헐(Enhanced Pause,Penh)표시.이PC100[기도반응성승고위생리염수(NS)치2배시적Mch격발농도]급Penh/NS%max종합평개기도반응성.수집지기관폐포관세액,도편후소목소-이홍염색계수기산립세포비례,폐조직병리검측.결과 1μg항조PC100(13.2±6.9)g/L、10/μg조(11.8±5.58)g/L여효천조(5.97±1.73)g/L상비,P<0.01표시차이유통계학의의;1 μg、10 μg조Penh/NS%max분별위(623.22±252.39)%,(519.71±200.41)%,현저저우효천조(1 306.83±540.46)%,P<0.01.10 μg조BALF기산립세포백분비위(42.75±7.44)%현저저우효천조(57.25±13.2)%,P<0.01,1 μg조、100 μg조BALF기산립세포백분비여효천조상비,차이무통계학의의.결론 잡개균다당핵산가이현저억제효천소서적기도고반응성급기도염증.
Objective To investigate the effect of Bacillus Calmette-Guérin polysaccharide nucleotide(BCG-PSN)on airway hyperresponsiveness and airway inflammation in asthmatic mouse model.Methods Balb/c mice at 6 weeks of age were sensitized with ovalbumin(OVA)by intraperitoneal injection on day 0,7 and 14,then challenged with OVA by intranasal administration on day 28,29,30 for asthmatic model.The mice of control group didn't receive any treatment.A pretreatment of 1 μg,10 μg,100 μg of BCG-PSN by intraperitoneal injection were done at 7 days before the first sensitization.Bronchial hyperresponsiveness,bronchoalveolar lavage fluid and histological changes in the airways were examined 48 hours after the final challenge.The data was processed statistically with one-way analysis of variance in SPSS package(version 13.0).Results Airway hyperresponsiveness of mice administrated with BCG-PSN at the dose of 1 μg,10 μg were significantly lower than the asthma group(P<0.05).The treatment with BCG-PSN at the dose of 100 μg didn't decrease airway hyperresponsiveness significantly.Airway eosinophilia of 10 μg group was significantly lower than the asthma group[(42.75±7.44)%)vs(57.25±13.19)%,P<0.01].Airway eosinophilia of 1 μg group,100 μg group[(49.13±4.99)%vs(52.75±7.34)%]both have no significant difference compared with asthma group(P>0.05).Conclusions BCG-PSN can inhibit the airway inflammation and decrease the hyperresponsiveness in asthmatic mouse model.
