CAJ | 학술논문

目的探讨不同浓度的亚砷酸(As2O3)、硼替佐米(BOR)单用及联合应用对恶性淋巴瘤细胞株Raji细胞周期的影响.方法采用流式细胞术(FCM)检测不同浓度梯度As2O3、BOR单用及联合应用对Raii细胞作用后,不同时间(24、48、72 h)G1、S期占整个细胞周期的比例以及凋亡率.结果 FCM检测细胞周期显示:As2O3使Raii细胞周期阻滞在G1期,使G1期细胞占细胞周期的比例明显增高,S期明显减少,呈现剂量、时间依赖效应(P＜0.0001),并出现明显凋亡峰.BOR对Raji细胞G1、S期所占比例与对照组相比差异无统计学意义(P＞0.05).而两药联合组与单用As2O3组亦无明显差异(P＞0.05).但两药联合组凋亡率由16.98%提高到45.84%.结论 As2O3通过阻抑细胞周期G1期来对Raji细胞发挥作用,与BOR两药联合促凋亡作用增强.
목적 탐토불동농도적아신산(As2O3)、붕체좌미(BOR)단용급연합응용대악성림파류세포주Raji세포주기적영향.방법 채용류식세포술(FCM)검측불동농도제도As2O3、BOR단용급연합응용대Raii세포작용후,불동시간(24、48、72 h)G1、S기점정개세포주기적비례이급조망솔.결과 FCM검측세포주기현시:As2O3사Raii세포주기조체재G1기,사G1기세포점세포주기적비례명현증고,S기명현감소,정현제량、시간의뢰효응(P＜0.0001),병출현명현조망봉.BOR대Raji세포G1、S기소점비례여대조조상비차이무통계학의의(P＞0.05).이량약연합조여단용As2O3조역무명현차이(P＞0.05).단량약연합조조망솔유16.98%제고도45.84%.결론 As2O3통과조억세포주기G1기래대Raji세포발휘작용,여BOR량약연합촉조망작용증강.
Objective To investigate the effect of bortezomib with arsenic trioxide different concentration on the cell cycle and apoptosis of Raji cells. Methods Flow cytometry analysis showed that the relative number of cells in different phases and the percentages of cells calculated in G1 and S phase of the cell cycle and apoptosis were assessed after treatment with As2O3 and BOR or in combination with BOR in different concentration at indicated time (24 h, 48 h, 72 h). Results Flow cytometric analysis showed that the cell cycle was arrested at G1 phase, the number of cells G1 period increased significantly, and S phase decreased on Raji cells after As2O3 treatment. The relationship between the cellular DNA contents and the concentration of As2O3 showed a dose-and time-dependent manner (P ＜0.0001). But it was found that BOR had no effect on Raji cell cycle, but, in two drugs combination, cell apoptosis rate significantly increased from 16.98 % to 45.84 %. Conclusion The results show that As2O3 exerted variable and definite effects on lymphoma Raji cells, which indicated that As2O3 might induce apoptosis and arrest cell cycle. The combination of two drugs had a effective and synergistic effect on apoptosis.