CAJ | 학술논문

目的观察补体片段C3f对人胚肺成纤维细胞(MRC-5)表达和分泌Ⅰ、Ⅲ型胶原及转化生长因子(TGF)-β1的影响.方法将人工合成的17肽片断C3f加入培养基中刺激MRC-5细胞,通过细胞免疫组化和ELISA法检测Ⅰ、Ⅲ型胶原及TGF-β1细胞内、外表达情况.结果随着C3f浓度的增加,MRC-5细胞上清液中Ⅰ、Ⅲ型胶原和TGF-β1的表达量逐渐降低,与对照组相比,差异有统计学意义(P＜0.05).MRC-5胞质中TGF-β1的表达量随C3f浓度增加降低趋势,与对照组相比,差异有统计学意义(P＜0.05).结论 C3f能够抑制MRC-5细胞中Ⅰ、Ⅲ型胶原和TGF-β1的形成,减轻肺组织的纤维化程度.
목적 관찰보체편단C3f대인배폐성섬유세포(MRC-5)표체화분비Ⅰ、Ⅲ형효원급전화생장인자(TGF)-β1적영향.방법 장인공합성적17태편단C3f가입배양기중자격MRC-5세포,통과세포면역조화화ELISA법검측Ⅰ、Ⅲ형효원급TGF-β1세포내、외표체정황.결과 수착C3f농도적증가,MRC-5세포상청액중Ⅰ、Ⅲ형효원화TGF-β1적표체량축점강저,여대조조상비,차이유통계학의의(P＜0.05).MRC-5포질중TGF-β1적표체량수C3f농도증가강저추세,여대조조상비,차이유통계학의의(P＜0.05).결론 C3f능구억제MRC-5세포중Ⅰ、Ⅲ형효원화TGF-β1적형성,감경폐조직적섬유화정도.
Objective To observe the effects of complement fragment C3f on expression and secretion of collagen[,Ⅲ and transforming growth factor(TGF)-β1 in human embryonic lung fibroblast(MRC-5)cells.Methods MRC-5 cells were cultured with C3f(the synthetic 17peptides fragments of complement C3).The extracellular and intracellular expression levels of type Ⅰ,Ⅲ collagens and TGF-β1 in MRC-5 cultures were detected by ELISA and immunohistochemistry,respectively.Results The expression levels of type Ⅰ,Ⅲcollagen and TGF-31 in the supematant of MRC-5 cultures decreased significantly with the concentrations of C3f as compared with controls(P＜0.05).Also the expression level of TGF-31 in MRC-5 cytoplasm reduced significantly as compared with controls(P＜0.05).Conclusion The results of present in vitro study showed that the complement fragment C3f could reduce the formation of TGF-β1 and type Ⅰ,Ⅲ collagens in MRC-5 cells,and inhibit the lung tissue fibrosis.