CAJ | 학술논문

目的筛选矽尘接触人群血清蛋白质指纹并分析其与接尘量的相关性.方法应用液体芯片飞行时间质谱技术检测Ⅰ期矽肺组(25例)、正常对照组(30例)、矽尘接触组(30例)及可疑矽肺组(30例)血清蛋白质指纹并筛选出差异显著的蛋白,对每个样品差异蛋白的峰强度与其对应的接尘量进行相关性分析.结果 4组间共筛选出5个差异显著蛋白,其中5081 Da、5066 Da为表达上调蛋白,3954 Da、2021 Da、1777 Da为表达下调蛋白,将这些蛋白峰强度分别与其相应的接尘量进行相关分析,未发现蛋白峰程度与接尘量有相关关系(r1=0.039,r2=0.104,r3=-0.047,r4=-0.028,r5=-0.016,P＞0.05).结论本次研究中矽尘接触人群血清蛋白质指纹与接尘量不存在相关关系.
목적 사선석진접촉인군혈청단백질지문병분석기여접진량적상관성.방법 응용액체심편비행시간질보기술검측Ⅰ기석폐조(25례)、정상대조조(30례)、석진접촉조(30례)급가의석폐조(30례)혈청단백질지문병사선출차이현저적단백,대매개양품차이단백적봉강도여기대응적접진량진행상관성분석.결과 4조간공사선출5개차이현저단백,기중5081 Da、5066 Da위표체상조단백,3954 Da、2021 Da、1777 Da위표체하조단백,장저사단백봉강도분별여기상응적접진량진행상관분석,미발현단백봉정도여접진량유상관관계(r1=0.039,r2=0.104,r3=-0.047,r4=-0.028,r5=-0.016,P＞0.05).결론 본차연구중석진접촉인군혈청단백질지문여접진량불존재상관관계.
Objective To explore the correlation between the exposure levels and serum protein fingerprints in population exposed to silica.Methods Liquid chip time-of-flight mass spectrometry technology was used to investigate the serum profiles in control group (30 cases),group exposed to silica (30 cases),silicosis group (Ⅰ stage,25 cases) and suspected silicosis group (30 cases),and screen the differential expression proteins.The correlation between the levels of the differential expression proteins and the exposure levels was performed.Results Five differential expression proteins were found among 4 groups,the expression of 5081 and 5066 proteins was upregulated,and the expression of 3954,2021 and 1777 proteins was downregulated.There was no the correlation between the exposure levels and the peak with M/Z among those proteins.Conclusion the results of present investigation indicated there was no correlation between thw exposure levels and protein/peptide peak.