CAJ | 학술논문

目的探讨急性坏死性胰腺炎(ANP)大鼠模型的腹腔内压力和TNF-α的时相改变及其规律.方法 80只SD大鼠按数字表法分为ANP组和对照组.采用4.5%牛磺胆酸钠胰管内逆行注射制备ANP模型,对照组注射等量生理盐水.制模后1、3、6、12、24 h分批各处死8只大鼠,检测血淀粉酶、TNF-α水平,测定腹水量和腹腔内压力,并观察胰腺病理改变.结果 ANP大鼠血淀粉酶含量呈进行性升高,24 h时高达对照组的32倍;血TNF-α水平也显著升高,6 h时达到峰值,为对照组的18倍;制模后1 h起腹水量开始增多,于24 h达到高峰,约为对照组4.7倍;制模后1 h起腹内压力明显升高,3 h时约为对照组的3倍,12 h约为对照组的9倍;胰腺病理损伤进行性加重,24 h损伤最严重.结论 ANP大鼠存在明显腹内高压,制模后12 h腹内压最高,炎症介质TNF-α升高高峰为制模后6 h,故腹内高压可能为炎症反应的后续反应.
목적 탐토급성배사성이선염(ANP)대서모형적복강내압력화TNF-α적시상개변급기규률.방법 80지SD대서안수자표법분위ANP조화대조조.채용4.5%우광담산납이관내역행주사제비ANP모형,대조조주사등량생리염수.제모후1、3、6、12、24 h분비각처사8지대서,검측혈정분매、TNF-α수평,측정복수량화복강내압력,병관찰이선병리개변.결과 ANP대서혈정분매함량정진행성승고,24 h시고체대조조적32배;혈TNF-α수평야현저승고,6 h시체도봉치,위대조조적18배;제모후1 h기복수량개시증다,우24 h체도고봉,약위대조조4.7배;제모후1 h기복내압력명현승고,3 h시약위대조조적3배,12 h약위대조조적9배;이선병리손상진행성가중,24 h손상최엄중.결론 ANP대서존재명현복내고압,제모후12 h복내압최고,염증개질TNF-α승고고봉위제모후6 h,고복내고압가능위염증반응적후속반응.
Objective To investigate the time-course change of intra-abdominal pressure (IAP), TNF-α in rat with acute necrotizing pancreatitis (ANP) and its mechanism. Methods 80 SD rats were randomly divided into ANP group and control group. ANP group was induced by retrograde injection of 4.5% sodium taurocholate into the pancreatic duct in SD rats, while control group was induced by injection of saline at the same dosage. Eight rats in each group were killed at 1 h, 3 h, 6 h, 12 h and 24 h, respectively. The serum levels of amylase, TNF-α were determined;IAP, amount of abdominal ascites, pancreatic pathologic changes were evaluated. Results The levels of amylase in ANP group increased progressively with time, which were 32-folds higher than those in control group at 24 h. The serum levels of TNF-α in ANP group reached the peak at 6 h, and were significantly higher than those in the control group. The amount of abdominal ascites increased post-ANP induction and reached the peak at 24 h, which was 4.7-folds higher than that in the control group. The IAP significantly increased 1 h after induction, which was 3 times as high as the control group in 3 h group and 9 times in 12 h group. Pathologic injuries deteriorated progressively and reached the peak at 24 h. Conclusions Significant IAP occurred in ANP rats and reached the peak at 12 h, TNF-α reached the peak at 6 h, therefore IAP may be the secondary response to inflammatory reaction.