中国医药
中國醫藥
중국의약
CHINA MEDICINE
2012年
11期
1433-1435
,共3页
人乳头瘤病毒%基因分型%基因芯片技术
人乳頭瘤病毒%基因分型%基因芯片技術
인유두류병독%기인분형%기인심편기술
Human papilloma virus%Gene chips%Genotyping
目的 调查泰安地区妇女子宫颈部人乳头瘤病毒(HPV)感染及基因亚型的分布情况,为该地区HPV亚型流行病学研究及宫颈癌防治提供理论依据.方法 选门诊及住院疑似HPV感染的宫颈炎患者675例,用专用宫颈刷采取子宫颈脱落细胞,提取DNA,用基因芯片技术检测HPV23种亚型.结果 HPV感染阳性者189例,感染率为28.00％.HPV阳性患者中高危亚型感染145例,占76.72％,低危亚型感染44例,感染率为23.28％.高危亚型感染率排在前6位的依次是16型(57例次,占21.96％)、52型(27例次,占9.93％)、66型(18例次,占6.62％)、33型(17例次,占6.25％)、58型(16例次,占5.88％)、18型(12例次,占4.41％)；低危亚型以43型(44例次,占16.18％)、11型(33例次,占12.13％)为主.单一基因型感染129例,占感染人数的68.25％;双重感染42例,占感染人数的22.22％；多重感染18例,占感染人数的9.52％.结论 泰安地区妇女宫颈HPV感染率较高,以单一亚型感染为主,HPV 16、43、11、52、66是泰安地区HPV感染的主要亚型.利用基因芯片技术对育龄女性进行HPV感染筛查及早治疗,并对持续高危亚型感染和混合亚型感染患者进行重点跟踪是防治宫颈癌的关键.
目的 調查泰安地區婦女子宮頸部人乳頭瘤病毒(HPV)感染及基因亞型的分佈情況,為該地區HPV亞型流行病學研究及宮頸癌防治提供理論依據.方法 選門診及住院疑似HPV感染的宮頸炎患者675例,用專用宮頸刷採取子宮頸脫落細胞,提取DNA,用基因芯片技術檢測HPV23種亞型.結果 HPV感染暘性者189例,感染率為28.00％.HPV暘性患者中高危亞型感染145例,佔76.72％,低危亞型感染44例,感染率為23.28％.高危亞型感染率排在前6位的依次是16型(57例次,佔21.96％)、52型(27例次,佔9.93％)、66型(18例次,佔6.62％)、33型(17例次,佔6.25％)、58型(16例次,佔5.88％)、18型(12例次,佔4.41％)；低危亞型以43型(44例次,佔16.18％)、11型(33例次,佔12.13％)為主.單一基因型感染129例,佔感染人數的68.25％;雙重感染42例,佔感染人數的22.22％；多重感染18例,佔感染人數的9.52％.結論 泰安地區婦女宮頸HPV感染率較高,以單一亞型感染為主,HPV 16、43、11、52、66是泰安地區HPV感染的主要亞型.利用基因芯片技術對育齡女性進行HPV感染篩查及早治療,併對持續高危亞型感染和混閤亞型感染患者進行重點跟蹤是防治宮頸癌的關鍵.
목적 조사태안지구부녀자궁경부인유두류병독(HPV)감염급기인아형적분포정황,위해지구HPV아형류행병학연구급궁경암방치제공이론의거.방법 선문진급주원의사HPV감염적궁경염환자675례,용전용궁경쇄채취자궁경탈락세포,제취DNA,용기인심편기술검측HPV23충아형.결과 HPV감염양성자189례,감염솔위28.00％.HPV양성환자중고위아형감염145례,점76.72％,저위아형감염44례,감염솔위23.28％.고위아형감염솔배재전6위적의차시16형(57례차,점21.96％)、52형(27례차,점9.93％)、66형(18례차,점6.62％)、33형(17례차,점6.25％)、58형(16례차,점5.88％)、18형(12례차,점4.41％)；저위아형이43형(44례차,점16.18％)、11형(33례차,점12.13％)위주.단일기인형감염129례,점감염인수적68.25％;쌍중감염42례,점감염인수적22.22％；다중감염18례,점감염인수적9.52％.결론 태안지구부녀궁경HPV감염솔교고,이단일아형감염위주,HPV 16、43、11、52、66시태안지구HPV감염적주요아형.이용기인심편기술대육령녀성진행HPV감염사사급조치료,병대지속고위아형감염화혼합아형감염환자진행중점근종시방치궁경암적관건.
Objective To investigate the infection situation and sub-genopype distribution of human papilloma virus (HPV) in female subjects in Taian area.Methods Exfoliated cells from 675 female subjects were harvested with specific cervical brush and DNA samples were prepared for gene chips analysis of 23 genotypes of HPV.Results Totally 189 samples were HPV infected and the percentage of positive HPV was 28.0％.18 subtypes of HPV were screened.High risk subtypes and low risk subtypes of HPV infection rates were 76.72％(145 cases) and 23.28％ (44 cases),respectively.Data also showed that the primary six high risk subtypes were HPV-16 (21.96％),HPV-52 (9.93％),HPV-66 (6.62％),HPV-33 (6.25％),HPV-58 (5.88％) and HPV-18(4.41％).The two main low risk subtypes of HPV were HPV-43 (16.18％) and HPV-11 (12.13％).129cases (68.25％) were single genotype infection; 42 cases (22.22％) and 18 cases (9.52％) were double genotypes infection and multiple genotypes infection,respectively.Conclusions Among female population in Taian area,the infection rate of HPV is 28.0％ and the primary infection manner is single genotype infection.The primary five high risk sub-geontypes are HPV-16,52,66,33 and 58.HPV infection screening and early-stage treament of female subjects employing gene chip methods,as well as follow-up on high risk sub-genotypes infected and mixed genotypes infected patients,are the key points of cervical cancer prevention.
