CAJ | 학술논문

目的评价不同剂量利多卡因对脓毒症大鼠小肠高迁移率组蛋白B1( HMGB1)表达的影响.方法成年雄性Wistar大鼠50只,体重200～250 g,采用随机数字表法,将大鼠随机分为5组(n=10):假手术组(S组)不行盲肠结扎穿孔术；脓毒症组(Sep组)采用盲肠结扎穿孔术法制备脓毒症模型；不同剂量利多卡因组(L1～3组)于术后即刻、1、2h时分别腹腔注射利多卡因5、10、20 mg/kg,S组和Sep组分别给予等容量生理盐水.于术后24、48 h时各组随机取5只大鼠处死取小肠组织,光镜下观察小肠组织病理学形态,采用PCR技术检测HMGB1 mRNA的表达,ELISA法测定二胺氧化酶(DAO)活性.结果与S组比较,Sep组和L1～3组各时点大鼠HMGB1 mRNA表达上调,DAO活性降低(P＜0.05)；与Sep组比较,L1～3组大鼠HMGB1 mRNA表达下调,DAO活性升高(P＜0.05)；各时点L1-3组大鼠HMGB1 mRNA表达依次下调,DAO活性依次升高(P＜0.05).L1-3组小肠组织病理学损伤较Sep组减轻.结论利多卡因可呈剂量依赖性减轻脓毒症大鼠小肠损伤,其机制可能与抑制HMGB1表达上调有关.
목적 평개불동제량리다잡인대농독증대서소장고천이솔조단백B1( HMGB1)표체적영향.방법 성년웅성Wistar대서50지,체중200～250 g,채용수궤수자표법,장대서수궤분위5조(n=10):가수술조(S조)불행맹장결찰천공술；농독증조(Sep조)채용맹장결찰천공술법제비농독증모형；불동제량리다잡인조(L1～3조)우술후즉각、1、2h시분별복강주사리다잡인5、10、20 mg/kg,S조화Sep조분별급여등용량생리염수.우술후24、48 h시각조수궤취5지대서처사취소장조직,광경하관찰소장조직병이학형태,채용PCR기술검측HMGB1 mRNA적표체,ELISA법측정이알양화매(DAO)활성.결과 여S조비교,Sep조화L1～3조각시점대서HMGB1 mRNA표체상조,DAO활성강저(P＜0.05)；여Sep조비교,L1～3조대서HMGB1 mRNA표체하조,DAO활성승고(P＜0.05)；각시점L1-3조대서HMGB1 mRNA표체의차하조,DAO활성의차승고(P＜0.05).L1-3조소장조직병이학손상교Sep조감경.결론 리다잡인가정제량의뢰성감경농독증대서소장손상,기궤제가능여억제HMGB1표체상조유관.
Objective To investigate the effect of different doses of lidocaine on expression of the high mobility group box 1 protein (HMGB1) in small intestine in septic rats.Methods Fifty adult male Wistar rats weighing 200-250 g were randomly divided into 5 groups ( n =10 each):sham operation group (group S),sepsis group( group Sep ) and different doses of lidocaine group (group L1～3 ).Group S were not applied cecal ligation and puncture (CLP).Sepsis intestinal damage model was performed in group Sep by CLP.Group L1～3 were given intraperitoneally lidocaine in a dose ofS,10 and 20 mg/kg at immediately,1 and 2 h after CLP,respectively.Isometric normal saline was given intraperitoneally in group S and group Sep.The small intestine tissues were taken at 24 and 48 h after CLP.The small intestine morphology was observed with optical microscope.The expression of the HMGB1 mRNA were examined by PCR and the activity of diamine oxidase (DAO) were determined by ELISA.Results Compared with group S,the expression of HMGB1 mRNA was increased and the activity of DAO decreased in group Sep and groups L1～3 at 24 and 48 h after CLP ( P ＜ 0.05 ).Compared with group Sep,the expression of HMGB1 mRNA was decreased and the activity of DAO increased in a dose-dependent manner in groups L1～3 ( P ＜0.05),The injury of pathology of small intestine was slighter in groups L1～3 than in group Sep.Conclusion Lidocaine can reduce samll intestine injury through inhibiting HMGB1 expression in septic rats by a dose-dependent manner.