CAJ | 학술논문

目的探讨体外培养条件下生精细胞向单倍体精子细胞分化以及精子细胞变形为精子的可能性.方法对11例非梗阻性无精子症患者行睾丸活检,将其中9份具有生精细胞的活检标本在终浓度为50 U/L卵泡刺激素和1μmol/L睾酮的改良人输卵管液培养液中进行体外培养,对培养前及培养后24h标本中精子及其他细胞进行计数,计算各种细胞的比例.应用流式细胞仪对2例患者培养前和培养后24 h的细胞进行细胞倍体分析.结果 9例标本培养前精子比例为(17.7±8.9)％,培养24h后为(25.6±10.3)％,培养前后差异有统计学意义(P=0.004).2例细胞倍体分析结果显示,培养前可见4、2和1 n波峰,培养后4 n波峰消失,1 n波峰显著增宽和增高.结论生精细胞体外培养可使生精细胞发生减数分裂,并使精子细胞向精子变形.
목적 탐토체외배양조건하생정세포향단배체정자세포분화이급정자세포변형위정자적가능성.방법 대11례비경조성무정자증환자행고환활검,장기중9빈구유생정세포적활검표본재종농도위50 U/L란포자격소화1μmol/L고동적개량인수란관액배양액중진행체외배양,대배양전급배양후24h표본중정자급기타세포진행계수,계산각충세포적비례.응용류식세포의대2례환자배양전화배양후24 h적세포진행세포배체분석.결과 9례표본배양전정자비례위(17.7±8.9)％,배양24h후위(25.6±10.3)％,배양전후차이유통계학의의(P=0.004).2례세포배체분석결과현시,배양전가견4、2화1 n파봉,배양후4 n파봉소실,1 n파봉현저증관화증고.결론 생정세포체외배양가사생정세포발생감수분렬,병사정자세포향정자변형.
Objective To investigate the possibility of differentiation and spermiogenesis of spermatocytes under in vitro condition. Methods Testis biopsy was done in 11 cases with non-obstructive azoospermia.Cells were prepared from 9 samples with spermatocytes and cultured in medium containing follicle stimulating hormone 50 U/L and testosterone 1 μmol/L.Sperms and cells of other types were counted and the proportion of every cell type was calculated before and 24 hours after culture.Flow cytometry was conducted before and 24 hours after culture in 2 cases to analyze the ploidy of the cells. Results The proportion of sperm in 9 samples was ( 17.7 ± 8.9 ) ％ before culture and ( 25.6 ± 10.3 ) ％ after culture ( P =0.004).Sperm increased significantly after culture.Flow cytometry demonstrated that the diagram of 4 n,2 n and 1 n converted to 2 n and broader 1 n. Conclusion Meiosis of spermatocytes and the transformation of spermatid into sperm could arise in in vitro culture.