中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2010年
2期
115-118
,共4页
嗅鞘细胞%神经干细胞%细胞增殖%细胞分化
嗅鞘細胞%神經榦細胞%細胞增殖%細胞分化
후초세포%신경간세포%세포증식%세포분화
Olfactory ensheathing cells%Neural stem cells%Cell proliferation%Cell differentiation
目的 观察嗅鞘细胞(OECs)对神经干细胞(NSCs)增殖、分化的影响.方法 新生大鼠脑OECs和NSCs原代培养,采用免疫荧光及免疫细胞化学方法鉴定相关细胞.取原代OECs分为2组,实验组去除培养孔的间隔,使OECs和NSCs共用一培养液体系;对照组不破坏培养孔的间隔,单独培养NSCs,其余同实验组.观察2组细胞增殖、分化情况.结果 原代培养的OECs表达神经生长因子受体(P75NGFR);原代培养的神经球表达巢蛋白(nestin),神经球分化的细胞表达神经丝200(NF200)和胶质纤维酸性蛋白(GFAP).增殖实验中,实验组NSCs数量较对照组明显增多,差异有统计学意义(P<0.05).诱导分化实验中,实验组4d、7d时NF200阳性细胞率较对照组明显升高,差异有统计学意义(P<0.05),说明2种细胞共液培养时,OECs提高了NSCs向NF200阳性细胞分化率.结论 OECs可促进NSCs增殖,并提高了NSCs向神经元分化的效率.
目的 觀察嗅鞘細胞(OECs)對神經榦細胞(NSCs)增殖、分化的影響.方法 新生大鼠腦OECs和NSCs原代培養,採用免疫熒光及免疫細胞化學方法鑒定相關細胞.取原代OECs分為2組,實驗組去除培養孔的間隔,使OECs和NSCs共用一培養液體繫;對照組不破壞培養孔的間隔,單獨培養NSCs,其餘同實驗組.觀察2組細胞增殖、分化情況.結果 原代培養的OECs錶達神經生長因子受體(P75NGFR);原代培養的神經毬錶達巢蛋白(nestin),神經毬分化的細胞錶達神經絲200(NF200)和膠質纖維痠性蛋白(GFAP).增殖實驗中,實驗組NSCs數量較對照組明顯增多,差異有統計學意義(P<0.05).誘導分化實驗中,實驗組4d、7d時NF200暘性細胞率較對照組明顯升高,差異有統計學意義(P<0.05),說明2種細胞共液培養時,OECs提高瞭NSCs嚮NF200暘性細胞分化率.結論 OECs可促進NSCs增殖,併提高瞭NSCs嚮神經元分化的效率.
목적 관찰후초세포(OECs)대신경간세포(NSCs)증식、분화적영향.방법 신생대서뇌OECs화NSCs원대배양,채용면역형광급면역세포화학방법감정상관세포.취원대OECs분위2조,실험조거제배양공적간격,사OECs화NSCs공용일배양액체계;대조조불파배배양공적간격,단독배양NSCs,기여동실험조.관찰2조세포증식、분화정황.결과 원대배양적OECs표체신경생장인자수체(P75NGFR);원대배양적신경구표체소단백(nestin),신경구분화적세포표체신경사200(NF200)화효질섬유산성단백(GFAP).증식실험중,실험조NSCs수량교대조조명현증다,차이유통계학의의(P<0.05).유도분화실험중,실험조4d、7d시NF200양성세포솔교대조조명현승고,차이유통계학의의(P<0.05),설명2충세포공액배양시,OECs제고료NSCs향NF200양성세포분화솔.결론 OECs가촉진NSCs증식,병제고료NSCs향신경원분화적효솔.
Objective To explore the effect of olfactory ensheathing cells (OECs) on the proliferation and differentiation of neural stem cells (NSCs). Methods The cells from the embryonic rat brain were primarily cultured and identified by immunofluorescence and immunocytochemistry. NSCs in the experimental group shared medium with OECs were cultured and induced to differentiate. Simultaneously, NSCs in the control group were cultured alone. The effect of OECs on the proliferation and differentiation of NSCs was observed by immunocytochemistry. Results Nerve growth factor receptor (P75NGFR) was observed in the primarily cultured OECs; nestin was expressed in the primarily cultured neurosphere and the cells differentiated from the neurosphere expressed neurofilament 200 (NF200) and glial fibrillary acidic protein (GFAP). Compared with that in the control group, the number of NSCs in the experimental group was significantly increased (P<0.05). On the 4~(th) and 7~(th) day of differentiation, the percentage of NF200-positive cell was higher in the experimental group than that in the control group (P<0.05), indicating that the appearance of OECs increased the differentiation of NSCs into NF200-positive cells. Conclusion OECs can promote the proliferation of NSCs and induce the differentiation of NSCs into neurons.
