CAJ | 학술논문

目的通过比较结肠腺癌与结肠腺瘤组织的蛋白质组表达差异,寻找结肠腺癌相关的蛋白质,筛选结肠癌早期诊断的分子标志物.方法应用蛋白质组学技术,对8例结肠腺癌组织和8例结肠腺瘤组织进行胶内差异双向电泳(2-D),选择差异表达超过2倍的蛋白点进行MALDI-TOF/TOF质谱分析和生物学信息分析.结果成功建立结肠腺癌和结肠腺瘤的双向凝胶电泳图谱,结肠腺癌和腺瘤组织凝胶电泳图谱中平均蛋白质斑点数分别为3289和2986,其中表达差异超过2倍的斑点共有31个,质谱分析和数据库检索共鉴定出18个蛋白质,包括keratin 8、S100A6、protein disulfide isomerase等.结论蛋白质组学可提示结肠腺癌与腺瘤组织间的蛋白质表达差异,而对相关差异表达蛋白的研究有可能为结肠癌的早期诊断和治疗提供新的分子标记物.
목적 통과비교결장선암여결장선류조직적단백질조표체차이,심조결장선암상관적단백질,사선결장암조기진단적분자표지물.방법 응용단백질조학기술,대8례결장선암조직화8례결장선류조직진행효내차이쌍향전영(2-D),선택차이표체초과2배적단백점진행MALDI-TOF/TOF질보분석화생물학신식분석.결과 성공건립결장선암화결장선류적쌍향응효전영도보,결장선암화선류조직응효전영도보중평균단백질반점수분별위3289화2986,기중표체차이초과2배적반점공유31개,질보분석화수거고검색공감정출18개단백질,포괄keratin 8、S100A6、protein disulfide isomerase등.결론 단백질조학가제시결장선암여선류조직간적단백질표체차이,이대상관차이표체단백적연구유가능위결장암적조기진단화치료제공신적분자표기물.
Objective To investigate the cancer associated proteins and sensitive biomakers for early diagnosis in colon adenocarcinoma by using proteomic technique.Methods Two-dimensional gel electrophoresis was used to define patterns of protein expression in adenocarcinoma tissue from 8 patients and 8 with adenomatous polyps.Proteins expressed differently of a 2-fold change were cut and analyzed by MALDI-TOF/TOF mass spectrometry.Results Two-dimensional protein maps of adenocarcinoma and adenoma were gained successfully.Gel-analysis software identified an average of 3289 spots in adenocarcinoma while 2986 in adenoma tissue and statistical filtering yielded 31 spots of a 2-fold change,18 of which were identified by using mass spectrometry,including keratin 8,S100A6,protein disulfide isomerase and so on.Conclusion Proteomic analysis can identify the proteins with variance of colon adenocarcinoma versus adenoma tissue as well as provide probable new biomakers correlated with biological behavior of colon adenocarcinoma.