CAJ | 학술논문

以大米蛋白为原料,研究其酶解工艺及其水解物的抗氧化活性.选取底物浓度、加酶量、酶解pH、酶解温度为考察因素,进行了酶解工艺的单因素及正交试验.试验结果表明,底物浓度([S])10%,加酶量([E] /[S])5%,酶解pH 6.0,酶解温度60℃,酶解时间90 min为最佳酶解参数,在此条件下大米蛋白水解物的固形物含量为25.6mg/mL,对DPPH自由基清除率为54.5%.抗氧化试验显示,大米蛋白水解物具有一定的清除DPPH自由基和羟自由基能力,其IC50分别为1.738和0.238mg/mL.大米蛋白水解物同样也具有较强的还原能力.由此得出,大米蛋白水解物是一种天然的抗氧化肽.
이대미단백위원료,연구기매해공예급기수해물적항양화활성.선취저물농도、가매량、매해pH、매해온도위고찰인소,진행료매해공예적단인소급정교시험.시험결과표명,저물농도([S])10%,가매량([E] /[S])5%,매해pH 6.0,매해온도60℃,매해시간90 min위최가매해삼수,재차조건하대미단백수해물적고형물함량위25.6mg/mL,대DPPH자유기청제솔위54.5%.항양화시험현시,대미단백수해물구유일정적청제DPPH자유기화간자유기능력,기IC50분별위1.738화0.238mg/mL.대미단백수해물동양야구유교강적환원능력.유차득출,대미단백수해물시일충천연적항양화태.
The enzymatic hydrolysis technology of rice protein and the antioxidation activity of the hydrolysate were studied. Substrate concentration, enzyme dose, pH value and temperature were selected as factors to optimize the hydroly-sis parameters with single - factor and orthogonal tests. Results show the optimum condition for the enzymatic hydrolysis is as follows: substrate concentration 10%, enzyme dose 5%, pH value 6.0, temperature 60 ℃, and time 90 min. Under the optimum condition, the soluble solid content of the hydrolysate is 25.6 mg/mL, and the DPPH free radical scavenging rate is 54.5%. Moreover, the result of an antioxidation experiment shows that the rice protein hydrolysate has very strong scavenging capabilities for DPPH free radical and hydroxyl radical, and their IC50 are 1. 738 and 0.238 mg/mL respective-ly, showing a very strong reducing ability as well. In a word, rice protein hydrolysate is a sort of natural peptides with an-tioxidation activity.