CAJ | 학술논문

目的通过两组实验数据平行对比探讨细胞因子临床检测数据偏差的潜在原因.方法在严格实验条件与操作的前提下,采用定量ELISA对短程与长程观察组临床标本进行检测,并对实验数据进行分析.结果短期观察组开腹手术与腔镜手术者IL-6测值均呈短暂一过性升高,与术前比较均有显著性差异(各时点比较P分别<0.01,0.01及0.05);但其标准差及变异系数CV(S/X-)值有依时性升高的趋势;对长期观察组IFN-a治疗结束及随访期间的IL-2、IL-4与IL-12三者检测均值与治疗前均值进行比较,其升降幅度分别为-0.7%与31.0%, -47.5%与-24.8%,以及50.5%与-0.5%.但因标准差(S)以及变异系数过大(61.8%-168.6%之间),各时点测值与治疗前之间均未见显著性差别(P均>0.05),结论不同患者对环境中理化与生物学因子刺激的反应各异,由此造成的测值波动将干扰对细胞因子科研与临床的评价.
목적 통과량조실험수거평행대비탐토세포인자림상검측수거편차적잠재원인.방법 재엄격실험조건여조작적전제하,채용정량ELISA대단정여장정관찰조림상표본진행검측,병대실험수거진행분석.결과 단기관찰조개복수술여강경수술자IL-6측치균정단잠일과성승고,여술전비교균유현저성차이(각시점비교P분별<0.01,0.01급0.05);단기표준차급변이계수CV(S/X-)치유의시성승고적추세;대장기관찰조IFN-a치료결속급수방기간적IL-2、IL-4여IL-12삼자검측균치여치료전균치진행비교,기승강폭도분별위-0.7%여31.0%, -47.5%여-24.8%,이급50.5%여-0.5%.단인표준차(S)이급변이계수과대(61.8%-168.6%지간),각시점측치여치료전지간균미견현저성차별(P균>0.05),결론불동환자대배경중이화여생물학인자자격적반응각이,유차조성적측치파동장간우대세포인자과연여림상적평개.
Objective To analyse the latent inqredients making the warps result in the clinical examination of the human beings multiple cytokins in sera.Methods examinating the clinical samples both short and long period observed groups using quantified ELISA on the status of the strict exsamination conditions and operation, and making statistical analysis for the exmaination data.Results short period observed groups consisit of 40 patients with symptomatic gallstone for elective cholesystectomy wich include 20 casese of open cholesystectomy (OC) and 20 cases of laparoscopic cholesystectomy (LC).The value of the IL-6 show transient rise, and there was significant defference compared with that before operation. (P<0.01,0.01 and 0.05 respective ); but the standard deviation and the value of the S/X- show the trendency to increasing relied on time. the long period observed group contains 28 cases of patient with chronic hepatitis C virus infection that treatment by IFN-a injection. The averages of IL-2,IL-4 and IL-12 detection values When the end of the treatment(24th weeeks of treatment) and following up investigationg (48th weeeks of begaining tretment), were increased by shawed as -0.7% & 31.0%, -47.5% $ -24.8%,and 50.5% & -0.5%.For the standard deviation and the S/X- coefficient rate was too large(between 61.8% and 168.6%), there were no significant difference (P were all >0.05) compared with that before treatment.Conclusion It could make the warps examination result of the human beings multiple cytokins in sera because of the patient's different responseve to stimulate of physics,chemistry and biology during the observation.