海洋科学进展
海洋科學進展
해양과학진전
JOURNAL OF OCEANOGRAPHY OF HUANGHAI & BOHAI SEAS
2009年
4期
469-476
,共8页
北冰洋%沉积物岩芯%厌氧细菌%空间分布
北冰洋%沉積物巖芯%厭氧細菌%空間分佈
북빙양%침적물암심%염양세균%공간분포
Arctic Ocean%sedimentary cores%anaerobic bacteria%distribution
用逐步稀释法对北冰洋研究区10个沉积物岩芯(计189份样品)的厌氧细菌(AAB)进行分析,用改进的ZoBell 2216号培养基,在4 ℃和25 ℃温度中分别培养3周以上,根据培养结果统计样品中厌氧细菌的检出率与含量,分析厌氧细菌含量的分布情况.结果表明,4 ℃时AAB检出率为85.71%,含量范围 0~2.40×10~9 个·g-1,平均4.42×10~7 个·g-1;25 ℃时检出率为93.05%,含量范围0~1.10×10~9 个·g-1,平均5.31×10~7 个·g-1,略高于4 ℃时的平均值.研究表明研究区岩芯中广布AAB;温度的提高,可能提高AAB的相关指标;沉积物深度增加可能有利于AAB生长,但太深也不利于AAB生长,呈中层增高现象,但并不规则;在一定水深范围内,水深增加也有利于AAB的生长.同时给出了研究区内不同海域AAB分布差异.
用逐步稀釋法對北冰洋研究區10箇沉積物巖芯(計189份樣品)的厭氧細菌(AAB)進行分析,用改進的ZoBell 2216號培養基,在4 ℃和25 ℃溫度中分彆培養3週以上,根據培養結果統計樣品中厭氧細菌的檢齣率與含量,分析厭氧細菌含量的分佈情況.結果錶明,4 ℃時AAB檢齣率為85.71%,含量範圍 0~2.40×10~9 箇·g-1,平均4.42×10~7 箇·g-1;25 ℃時檢齣率為93.05%,含量範圍0~1.10×10~9 箇·g-1,平均5.31×10~7 箇·g-1,略高于4 ℃時的平均值.研究錶明研究區巖芯中廣佈AAB;溫度的提高,可能提高AAB的相關指標;沉積物深度增加可能有利于AAB生長,但太深也不利于AAB生長,呈中層增高現象,但併不規則;在一定水深範圍內,水深增加也有利于AAB的生長.同時給齣瞭研究區內不同海域AAB分佈差異.
용축보희석법대북빙양연구구10개침적물암심(계189빈양품)적염양세균(AAB)진행분석,용개진적ZoBell 2216호배양기,재4 ℃화25 ℃온도중분별배양3주이상,근거배양결과통계양품중염양세균적검출솔여함량,분석염양세균함량적분포정황.결과표명,4 ℃시AAB검출솔위85.71%,함량범위 0~2.40×10~9 개·g-1,평균4.42×10~7 개·g-1;25 ℃시검출솔위93.05%,함량범위0~1.10×10~9 개·g-1,평균5.31×10~7 개·g-1,략고우4 ℃시적평균치.연구표명연구구암심중엄포AAB;온도적제고,가능제고AAB적상관지표;침적물심도증가가능유리우AAB생장,단태심야불리우AAB생장,정중층증고현상,단병불규칙;재일정수심범위내,수심증가야유리우AAB적생장.동시급출료연구구내불동해역AAB분포차이.
189 samples divided from 10 sedimentary cores collected in the studied area of the Arctic Ocean are analyzed for anaerobic bacteria (abr. AAB) detection by means of the step-by-step dilution method. The samples respectively with the 4℃ temperature and the 25℃ have been cultured for 3 weeks to deter-mine the AAB-detected rate and the content in the samples and to analyze the AAB distribution. As it is shown from the results, the AAB-detected rate of the 4 ℃ samples is 85. 71% and the AAB contents range between 0 and 2. 40×10~9 ind. /g with an average of 4. 42×10~7 ind. /g, and the rate of the 25℃ is 93. 05% and the contents range between 0 and 1. 10 ind. /g with their average of 5. 31×10~7 ind. /g, slightly higher than the 4 ℃ average. Therefore AAB is widely distributed in the cores from the studied area. It seems that the temperature increment is helpful for the increments of those indices. In addition, the sedimentary depths within the centain limit might be favourable to AAB growth. In general, the AAB contents become higher in the middle layers although it is not definitely regular. Water depth within a certain limit in the ocean might be also advantageous to AAB growth. The difference among the AAB regional distributions in the studied area is shown in the paper.