CAJ | 학술논문

叶绿素是光合作用中最重要的色素,与大豆籽粒产量密切相关.本研究采用溧水中子黄豆×南农493-1后代衍生的244个F_2单株及筛选的150个SSR分子标记构建的连锁遗传图谱,在苗期至开花期测定F_2衍生F_(2:3)和F_(2:4)家系生长正常单株的倒3复叶功能叶(非离体)的叶绿素含量13次,通过Windows QTL Cartographer v2.5软件包的复合区间法,动态定位了大豆叶绿素含量的QTL.结果表明,不同时间点共检测到20个QTL,其中,不同发育阶段间、年份间和地点间共同的QTL较少,不同时间点上的QTL差异较大,重复出现在N、Dla、F和K连锁群的QTL有3～4次.这些结果为叶绿素含量的遗传剖析和标记辅助育种提供理论依据.
협록소시광합작용중최중요적색소,여대두자립산량밀절상관.본연구채용률수중자황두×남농493-1후대연생적244개F_2단주급사선적150개SSR분자표기구건적련쇄유전도보,재묘기지개화기측정F_2연생F_(2:3)화F_(2:4)가계생장정상단주적도3복협공능협(비리체)적협록소함량13차,통과Windows QTL Cartographer v2.5연건포적복합구간법,동태정위료대두협록소함량적QTL.결과표명,불동시간점공검측도20개QTL,기중,불동발육계단간、년빈간화지점간공동적QTL교소,불동시간점상적QTL차이교대,중복출현재N、Dla、F화K련쇄군적QTL유3～4차.저사결과위협록소함량적유전부석화표기보조육충제공이론의거.
Chlorophyll is the most important photosynthetic pigment and closely related to soybean seed yield. However, there are still very few studies at different developmental stages under multiple environments. A genetic linkage map using 244 F_2 plants derived from a soybean cross between Lishuizhongzihuang and Nannong 493-1 was constructed. The F_(2:3) and F_(2:4) families were used to dissect the developmental behavior for chlorophyll content across different environments by quantitative trait locus (QTL) mapping approach. Chlorophyll contents for the function leaf (in vivo) in the 244 F_(2:3)and F_(2:4) families from seedling to blooming stages at Jiangpu and/or Linyi experimental stations in 2007 and/or 2008 were measured by SPAD-502 chlorophyll instrument. Composite interval mapping (CIM) of Windows QTL Cartographer v2.5 was used for the QTL analysis. A total of 20 QTLs were detected at various developmental stages under the two environments. However, there were few common QTLs identified across different developmental stages, years, and experimental stations, although there were 3 to 4 QTLs detected on each of the N, Dla, F, and K linkage groups. In the two years, one common QTL, qchl-Dla-1, located between markers sat_160 and satt147 on the linkage group Dla, was identified at Jiangpu experimental station. At the two experimental stations above, three common QTLs on the K, M, and N linkage groups were mapped. These results provide a theoretical basis for genetic analysis of chlorophyll traits and marker-assisted breeding.